Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACMX2G_RS00120 Genome accession   NZ_CP180375
Coordinates   18158..18766 (+) Length   202 a.a.
NCBI ID   WP_003153521.1    Uniprot ID   -
Organism   Bacillus velezensis strain NJN-6     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 13158..23766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMX2G_RS00080 (ACMX2G_00080) - 14211..14717 (-) 507 WP_003153536.1 PTS glucose transporter subunit IIA -
  ACMX2G_RS00085 (ACMX2G_00085) - 14856..15269 (+) 414 WP_003153535.1 hypothetical protein -
  ACMX2G_RS00090 (ACMX2G_00090) - 15299..15733 (-) 435 WP_003153534.1 YppG family protein -
  ACMX2G_RS00095 (ACMX2G_00095) - 15920..16102 (+) 183 WP_003153532.1 YppF family protein -
  ACMX2G_RS00100 (ACMX2G_00100) - 16141..16509 (-) 369 WP_003153529.1 YppE family protein -
  ACMX2G_RS00105 (ACMX2G_00105) - 16555..16800 (-) 246 WP_014305286.1 DUF5446 family protein -
  ACMX2G_RS00110 (ACMX2G_00110) sspM 17006..17110 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  ACMX2G_RS00115 (ACMX2G_00115) - 17154..18116 (-) 963 WP_041482374.1 DUF2515 domain-containing protein -
  ACMX2G_RS00120 (ACMX2G_00120) recU 18158..18766 (+) 609 WP_003153521.1 Holliday junction resolvase RecU Machinery gene
  ACMX2G_RS00125 (ACMX2G_00125) - 18805..21588 (+) 2784 WP_014305288.1 PBP1A family penicillin-binding protein -
  ACMX2G_RS00130 (ACMX2G_00130) - 21665..22156 (-) 492 WP_014305289.1 YpoC family protein -
  ACMX2G_RS00135 (ACMX2G_00135) nth 22153..22812 (-) 660 WP_003153517.1 endonuclease III -
  ACMX2G_RS00140 (ACMX2G_00140) dnaD 22831..23529 (-) 699 WP_003153516.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=1095442 ACMX2G_RS00120 WP_003153521.1 18158..18766(+) (recU) [Bacillus velezensis strain NJN-6]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=1095442 ACMX2G_RS00120 WP_003153521.1 18158..18766(+) (recU) [Bacillus velezensis strain NJN-6]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
CTACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATCGATTTCGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AAGAACGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.955

98.515

0.896

Multiple sequence alignment