Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACGHR8_RS16280 Genome accession   NZ_AP031445
Coordinates   3228167..3229555 (-) Length   462 a.a.
NCBI ID   WP_002583787.1    Uniprot ID   A0A1I2WQU9
Organism   Enterocloster clostridioformis strain k19-0097-F3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3223167..3234555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHR8_RS16255 (K190097F3_32080) - 3223323..3223898 (-) 576 WP_002583783.1 TRAP transporter small permease -
  ACGHR8_RS16260 (K190097F3_32090) - 3223964..3225058 (-) 1095 WP_057573221.1 TRAP transporter substrate-binding protein -
  ACGHR8_RS16265 (K190097F3_32100) - 3225117..3225800 (-) 684 WP_002583785.1 GntR family transcriptional regulator -
  ACGHR8_RS16270 (K190097F3_32110) - 3226701..3227690 (+) 990 WP_002583786.1 transposase -
  ACGHR8_RS16280 (K190097F3_32120) radA 3228167..3229555 (-) 1389 WP_002583787.1 DNA repair protein RadA Machinery gene
  ACGHR8_RS16285 (K190097F3_32130) - 3229642..3230058 (-) 417 WP_002567200.1 hypothetical protein -
  ACGHR8_RS16290 (K190097F3_32140) - 3230177..3232762 (-) 2586 WP_002583788.1 ATP-dependent Clp protease ATP-binding subunit -
  ACGHR8_RS16295 (K190097F3_32150) - 3232816..3233850 (-) 1035 WP_002583789.1 ATP--guanido phosphotransferase -
  ACGHR8_RS16300 (K190097F3_32160) - 3233843..3234460 (-) 618 WP_002583790.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 49961.49 Da        Isoelectric Point: 5.6461

>NTDB_id=109496 ACGHR8_RS16280 WP_002583787.1 3228167..3229555(-) (radA) [Enterocloster clostridioformis strain k19-0097-F3]
MAKAKTTAFFCKECGYESSKWMGQCPACKAWNSMVEEPVSKSAGSQGGLGRLSSGSQKSPIPAAKPSLLSEIDIEEQDRI
STGFGELDRVLGDGIVAGSLVLVGGDPGIGKSTLLLQVCRNLAGAGQRVLYISGEESLKQIKMRANRIGPVTGELKFLCE
TSLEHIERAIDTENPQIAVIDSIQTMYREEISSAPGSVSQVRESTGILMQIAKSRGIAIFVVGHVTKEGVVAGPRVLEHM
VDTVLYFEGDRNASYRILRSVKNRFGSTNEIGVFEMQEQGLAEVENPSEYMLDGRPEEASGAVVSCSFEGTRPILLEVQA
LVTETNFGMPRRTAAGTDYNRVNLLMAVLEKRCRYEMSRLDAYVNIAGGMKMNEPALDLAIIMALMSSYKDRPVDSKMLI
FGEVGLSGEVRAVSQAGQRVNEAAKLGFTTCVLPKVCADKMKQVEGIRLIGVSNVREAIGII

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=109496 ACGHR8_RS16280 WP_002583787.1 3228167..3229555(-) (radA) [Enterocloster clostridioformis strain k19-0097-F3]
ATGGCAAAAGCGAAAACCACCGCATTTTTCTGTAAGGAATGCGGATATGAATCATCCAAATGGATGGGGCAGTGTCCTGC
CTGCAAAGCCTGGAATTCCATGGTGGAGGAACCGGTGTCAAAGTCAGCTGGCAGTCAGGGCGGTTTAGGGCGCCTGTCTT
CGGGAAGCCAGAAATCCCCGATTCCGGCAGCCAAACCATCCCTTCTATCAGAGATTGACATAGAGGAGCAGGACCGTATA
TCCACGGGCTTTGGAGAATTGGACCGGGTGCTGGGAGATGGAATCGTGGCCGGCTCCCTGGTGCTGGTGGGCGGAGATCC
GGGTATCGGAAAATCCACCCTTCTGCTTCAGGTATGCCGGAATCTGGCAGGCGCGGGACAGCGGGTCCTGTACATATCAG
GGGAGGAATCCCTGAAACAGATTAAAATGAGGGCCAACCGCATTGGCCCGGTAACCGGAGAGCTGAAATTCCTGTGTGAG
ACAAGCCTGGAGCATATTGAGAGGGCCATTGATACTGAGAATCCCCAGATTGCAGTCATTGACTCCATACAGACCATGTA
CAGGGAGGAGATATCCTCGGCTCCCGGAAGCGTCAGCCAGGTAAGGGAGTCCACGGGAATCCTGATGCAGATAGCAAAGA
GCCGCGGAATTGCCATTTTCGTGGTGGGGCATGTGACAAAGGAAGGCGTGGTGGCAGGCCCAAGGGTATTGGAACATATG
GTGGATACGGTACTTTATTTTGAGGGAGACAGGAATGCGTCCTACCGCATTCTGCGGAGTGTGAAGAACCGGTTTGGCTC
TACCAATGAGATAGGTGTATTTGAGATGCAGGAACAGGGGCTGGCGGAGGTGGAGAATCCCTCCGAATATATGCTGGACG
GCCGGCCTGAGGAAGCCTCCGGCGCAGTGGTGTCCTGTTCCTTTGAGGGCACCAGGCCCATCCTTTTAGAGGTACAGGCC
CTGGTGACGGAAACCAATTTCGGTATGCCCAGGCGAACAGCGGCCGGTACGGATTACAACCGTGTCAACCTGCTGATGGC
CGTGCTGGAGAAGCGCTGCCGCTATGAGATGTCGCGGCTGGACGCCTATGTGAACATCGCGGGAGGTATGAAGATGAACG
AGCCCGCCCTGGACCTGGCCATTATCATGGCCCTTATGTCCAGCTACAAGGACAGGCCCGTGGATTCAAAGATGCTTATA
TTCGGGGAGGTGGGCCTGTCTGGAGAGGTCAGGGCCGTATCCCAGGCCGGCCAGCGTGTGAATGAGGCTGCCAAGCTGGG
TTTTACCACCTGTGTGCTGCCAAAGGTATGCGCGGATAAGATGAAGCAGGTGGAGGGCATACGCCTGATTGGAGTTTCCA
ATGTGCGGGAGGCCATTGGAATCATATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-36)

Subunit ChlI of Mg-chelatase (ChlI)

(351-435)

AAA domain (AAA_25)

(86-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I2WQU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

56.209

99.351

0.558
  radA Streptococcus mitis NCTC 12261

52.07

99.351

0.517
  radA Streptococcus pneumoniae Rx1

52.07

99.351

0.517
  radA Streptococcus pneumoniae D39

52.07

99.351

0.517
  radA Streptococcus pneumoniae R6

52.07

99.351

0.517
  radA Streptococcus pneumoniae TIGR4

52.07

99.351

0.517
  radA Streptococcus mitis SK321

52.07

99.351

0.517

Multiple sequence alignment