Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACLBK2_RS21250 Genome accession   NZ_CP180236
Coordinates   4081795..4082838 (-) Length   347 a.a.
NCBI ID   WP_048531150.1    Uniprot ID   A0A8T9Z920
Organism   Bacillus sp. Ba 3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4076795..4087838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBK2_RS21205 (ACLBK2_21205) - 4077222..4077422 (-) 201 WP_000106081.1 YqzE family protein -
  ACLBK2_RS21210 (ACLBK2_21210) aroK 4077461..4077958 (-) 498 WP_048531135.1 shikimate kinase AroK -
  ACLBK2_RS21215 (ACLBK2_21215) - 4078077..4078727 (-) 651 WP_166688051.1 2OG-Fe(II) oxygenase -
  ACLBK2_RS21220 (ACLBK2_21220) comGG 4078905..4079276 (-) 372 WP_247998525.1 competence type IV pilus minor pilin ComGG -
  ACLBK2_RS21225 (ACLBK2_21225) comGF 4079273..4079743 (-) 471 WP_166688050.1 competence type IV pilus minor pilin ComGF -
  ACLBK2_RS21230 (ACLBK2_21230) comGE 4079713..4080015 (-) 303 WP_071757286.1 competence type IV pilus minor pilin ComGE -
  ACLBK2_RS21235 (ACLBK2_21235) comGD 4080008..4080463 (-) 456 WP_048531144.1 comG operon protein ComGD -
  ACLBK2_RS21240 (ACLBK2_21240) comGC 4080460..4080759 (-) 300 WP_016086289.1 comG operon protein ComGC -
  ACLBK2_RS21245 (ACLBK2_21245) comGB 4080771..4081802 (-) 1032 WP_053082876.1 competence type IV pilus assembly protein ComGB -
  ACLBK2_RS21250 (ACLBK2_21250) comGA 4081795..4082838 (-) 1044 WP_048531150.1 competence protein ComGA Machinery gene
  ACLBK2_RS21255 (ACLBK2_21255) - 4083044..4083739 (+) 696 WP_166688048.1 helix-turn-helix transcriptional regulator -
  ACLBK2_RS21260 (ACLBK2_21260) - 4083865..4084107 (+) 243 WP_000440719.1 DUF2626 domain-containing protein -
  ACLBK2_RS21265 (ACLBK2_21265) - 4084215..4085609 (+) 1395 WP_166688047.1 L-cystine transporter -
  ACLBK2_RS21270 (ACLBK2_21270) - 4085826..4086041 (-) 216 WP_071757284.1 DUF3912 family protein -
  ACLBK2_RS21275 (ACLBK2_21275) - 4086295..4086606 (+) 312 WP_001093241.1 hypothetical protein -
  ACLBK2_RS21280 (ACLBK2_21280) - 4086646..4087134 (-) 489 WP_166688046.1 hypothetical protein -
  ACLBK2_RS21285 (ACLBK2_21285) - 4087214..4087588 (-) 375 WP_071757282.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39378.84 Da        Isoelectric Point: 8.8395

>NTDB_id=1094856 ACLBK2_RS21250 WP_048531150.1 4081795..4082838(-) (comGA) [Bacillus sp. Ba 3]
MNGIESFANMILKEACRVQASDLHIVPRKKDVAVQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYEHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1094856 ACLBK2_RS21250 WP_048531150.1 4081795..4082838(-) (comGA) [Bacillus sp. Ba 3]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAGGCGTGTAGGGTACAAGCTTCGGATTTACACATCGTGCC
CCGGAAGAAGGATGTTGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCATTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTTCTTTCCTTTTTAC
ATTATTCGCATGGGTTACTCGTCTTTACGGGACCGACTGGTTCAGGAAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTGAAGGCTATTTTACGCCATGATCCAGATATTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCAAAAATTGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAAAGGAGCGATACTTCGGTTTATGGACTTTGGCATAACAAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AGGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACGAGCATGAGACATTAGAATCTTCGCTACGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTGTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366

Multiple sequence alignment