Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACLBKY_RS05250 Genome accession   NZ_CP180233
Coordinates   1038130..1041834 (+) Length   1234 a.a.
NCBI ID   WP_414835206.1    Uniprot ID   -
Organism   Bacillus altitudinis strain Ba 5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1033130..1046834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBKY_RS05235 (ACLBKY_05235) - 1033428..1033838 (+) 411 WP_073415055.1 DoxX family protein -
  ACLBKY_RS05240 (ACLBKY_05240) - 1034030..1034476 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  ACLBKY_RS05245 (ACLBKY_05245) addB 1034643..1038152 (+) 3510 WP_394157935.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACLBKY_RS05250 (ACLBKY_05250) addA 1038130..1041834 (+) 3705 WP_414835206.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACLBKY_RS05255 (ACLBKY_05255) sbcD 1041860..1043035 (+) 1176 WP_008348619.1 exonuclease subunit SbcD -
  ACLBKY_RS05260 (ACLBKY_05260) sbcC 1043032..1046427 (+) 3396 WP_414835207.1 exonuclease subunit SbcC -
  ACLBKY_RS05265 (ACLBKY_05265) - 1046445..1046744 (+) 300 WP_414835208.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142831.78 Da        Isoelectric Point: 5.0967

>NTDB_id=1094723 ACLBKY_RS05250 WP_414835206.1 1038130..1041834(+) (addA) [Bacillus altitudinis strain Ba 5]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFETLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADNKETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTSEMLLVHLDQHDTENDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LKEDMSLEKQKQDEVVQALMEGVPVQGYGDYEEEVKERLTWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKTR
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTVIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGNLYLLDYKTDRVTGRFSGGLEEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1094723 ACLBKY_RS05250 WP_414835206.1 1038130..1041834(+) (addA) [Bacillus altitudinis strain Ba 5]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTGATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGATCACTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAAGTTCTCCGTACCTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTACTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCACATCCAGCGCCCGAACAGTGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAATTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGCCAGGAACTGCTGGAACAAGCG
CTCGCTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGAGACACTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCAGCTTTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGATCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAAAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCGGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAATACTATCAACAGCAA
TTTGAAGAAGTACTTGTGGATGAATACCAGGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTAGTGTCTAAAGGGAA
ACATCCTGCCGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGCAGATAACAAAGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGC
AGCCGGGCTGATGTATTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGTCAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAACGATGAAGAACGAGAAGAGCTAGAGACGGTACAATTTGAGGCGAGAGTGATTGCGAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGCTCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGTTATTTTGAAGCTACAGAAGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCACTAATTAGAACAAGTGA
TAAAAAAGGCACTTATTATGACGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACATGCAGAAAGCTGG
AGCGTTTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGTAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGGCTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTGTTTACTGCAGGACTTGGCAGGAACTTTAACATGATGGATTTAAACCAGTCCTATTTGCTCGA
TAAAGAATTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAACTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAGGAAAAATTA
TTTTTAGTCGGCTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGATGACTGGCTTCT
TCCTGATTTTGAACGATATCAAGCGAGAACATACTTAGATTTTATTGGCCCAGCCCTTATCCGTCATCAAGCGATGTCTT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCGCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTAAAAGAAGACATGTCACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGTGTGCCTGTTCAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAGAAAGACTTACATGGCGTTATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCTGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTTCTTCCATCCGAAAGACTCGT
GCGACACTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGTTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAATCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAAGCGATGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAAAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.613

100

0.668

Multiple sequence alignment