Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMVYT_RS09520 Genome accession   NZ_CP180209
Coordinates   2096468..2097841 (+) Length   457 a.a.
NCBI ID   WP_009892524.1    Uniprot ID   A0AB74R7U4
Organism   Clostridioides difficile strain 500/12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2091468..2102841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMVYT_RS09500 (ACMVYT_09500) - 2091848..2092312 (+) 465 WP_003425587.1 CtsR family transcriptional regulator -
  ACMVYT_RS09505 (ACMVYT_09505) - 2092319..2092813 (+) 495 WP_003421255.1 UvrB/UvrC motif-containing protein -
  ACMVYT_RS09510 (ACMVYT_09510) - 2092829..2093854 (+) 1026 WP_004453979.1 protein arginine kinase -
  ACMVYT_RS09515 (ACMVYT_09515) - 2093841..2096288 (+) 2448 WP_003425582.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMVYT_RS09520 (ACMVYT_09520) radA 2096468..2097841 (+) 1374 WP_009892524.1 DNA repair protein RadA Machinery gene
  ACMVYT_RS09525 (ACMVYT_09525) disA 2097844..2098914 (+) 1071 WP_004453975.1 DNA integrity scanning diadenylate cyclase DisA -
  ACMVYT_RS09530 (ACMVYT_09530) - 2099003..2100097 (+) 1095 WP_009887799.1 PIN/TRAM domain-containing protein -
  ACMVYT_RS09535 (ACMVYT_09535) - 2100226..2102403 (+) 2178 WP_009887801.1 hypothetical protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50273.11 Da        Isoelectric Point: 7.9863

>NTDB_id=1094545 ACMVYT_RS09520 WP_009892524.1 2096468..2097841(+) (radA) [Clostridioides difficile strain 500/12]
MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVSINSIESKEEERFTTDINELDRV
LGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGE
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIP
KRTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTGEVGLTGEV
RAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAINIVLGRNQ

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1094545 ACMVYT_RS09520 WP_009892524.1 2096468..2097841(+) (radA) [Clostridioides difficile strain 500/12]
ATGGCAAAAATAAAAACAAAATATGTATGTCAATCTTGTGGATATGAAACAGCTAAATGGCTTGGTAAATGTCCAGAATG
TACAAAATGGAATACATTTGTAGAAGAAATAGACCAAAAAAGCACCAAAAAAGAGGTCTTTATAATAGATAAATCTTCAT
CTAAACCAGTCAGTATAAACTCAATAGAAAGTAAAGAAGAAGAGAGGTTTACAACAGATATAAATGAATTAGATAGAGTG
CTTGGAGGAGGTATAGTTAAAGGCTCTTTAGTGCTTGTTGGTGGTGACCCTGGTATAGGTAAATCAACACTTTTAATCCA
AGTATCTAGTAATGTTGCCAATTTAGGAAAAACTGTTTTATATATAACTGGAGAAGAATCAGAATCTCAAATAAAAATGA
GAGCTAAAAGATTAGGTATTAACTCAGAAAATTTATATATATTTGCTGAAAATAATTTAAGTATAATTGAATCTTATTTA
GAAAGTGTAAATCCAGAATTAATAATTCTAGACTCAATTCAAACTGTCTTCAGTCCAGAAATATCCTCGGCACCAGGAAC
TGTCAGCCAAATTAAAGAAGGTACTTCAAAATTCATGAAAATTTCTAAAAAAATGGGAATTTCAACGTTCATAGTTGGGC
ATGTTACAAAAGAAGGTTCACTAGCAGGACCAAAATTATTAGAGCATATGGTTGATACTGTTCTATATTTTGAAGGAGAA
AGATATAATACTTATAGATTAGTAAGAGCTGTTAAAAATCGATTTGGTTCAACTAATGAACTTGGAGTATTTGAAATGAG
GGATTTAGGATTAGTAGAACTTGATAATCCATCTAAAATTTTAATCTCTGAAAAACCAAAAGATGTAGCAGGTTCAGTAA
TAATATCTACTGTAGAAGGTACGAGACCAATGTTACTTGAATTGCAAGCATTAGTTTCTCCTACAAGTTTTGGTATACCA
AAAAGAACATCTACAGGAGTCGATTACAATAGAGTAGGTATGTTATTAGCAGTATTAGAAAAACGTGTTGGTTTGCAAAT
ACAAAATCAAGATGTCTATATAAACATAGTTGGAGGAATAAAGATAAATGAACCCTCAATTGATTTAGGGATAGCCATTT
CTGTAGCATCTAGTTTTAGAAATATACCGATAGATGAAAATATAGCTGTTACAGGAGAAGTGGGACTTACAGGAGAAGTT
AGAGCAGTAAGCTTTATAGAAAAGAGAATAGCTGAGTGTAAAAAACTTGGATTTAAAAAGATAGTAGTTCCTAGAAGTAA
TTATGATGTTGTAAAAGAGACCAAAGGCATAGAAATATGGCCAGTAGATAACTTAAGACAAGCTATAAATATAGTACTTG
GGAGGAATCAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-219)

Subunit ChlI of Mg-chelatase (ChlI)

(342-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

57.112

100

0.571
  radA Streptococcus mitis NCTC 12261

51.101

99.344

0.508
  radA Streptococcus pneumoniae Rx1

51.101

99.344

0.508
  radA Streptococcus pneumoniae D39

51.101

99.344

0.508
  radA Streptococcus pneumoniae R6

51.101

99.344

0.508
  radA Streptococcus pneumoniae TIGR4

51.101

99.344

0.508
  radA Streptococcus mitis SK321

50.881

99.344

0.505

Multiple sequence alignment