Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACK8HE_RS01915 Genome accession   NZ_CP180192
Coordinates   401718..403151 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain K_21_ENCL_294     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396718..408151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8HE_RS01885 (ACK8HE_01885) elbB 398250..398903 (+) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACK8HE_RS01890 (ACK8HE_01890) mtgA 398900..399625 (+) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACK8HE_RS01895 (ACK8HE_01895) npr 399665..399937 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  ACK8HE_RS01900 (ACK8HE_01900) rapZ 399934..400788 (-) 855 WP_003861870.1 RNase adapter RapZ -
  ACK8HE_RS01905 (ACK8HE_01905) ptsN 400834..401325 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACK8HE_RS01910 (ACK8HE_01910) hpf 401408..401695 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  ACK8HE_RS01915 (ACK8HE_01915) rpoN/rpoN1 401718..403151 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  ACK8HE_RS01920 (ACK8HE_01920) lptB 403199..403924 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  ACK8HE_RS01925 (ACK8HE_01925) lptA 403931..404485 (-) 555 WP_017693908.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACK8HE_RS01930 (ACK8HE_01930) lptC 404454..405029 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  ACK8HE_RS01935 (ACK8HE_01935) kdsC 405026..405592 (-) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACK8HE_RS01940 (ACK8HE_01940) kdsD 405607..406593 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  ACK8HE_RS01945 (ACK8HE_01945) - 406607..407584 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=1094382 ACK8HE_RS01915 WP_003861864.1 401718..403151(-) (rpoN/rpoN1) [Enterobacter hormaechei strain K_21_ENCL_294]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1094382 ACK8HE_RS01915 WP_003861864.1 401718..403151(-) (rpoN/rpoN1) [Enterobacter hormaechei strain K_21_ENCL_294]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTCCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTAGACTCCGTAGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGATGA
TGAACTCCCGGTCTACCAGGGCGAAACCACTCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTCCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAGGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment