Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMUKE_RS10475 Genome accession   NZ_CP180178
Coordinates   2222487..2223860 (-) Length   457 a.a.
NCBI ID   WP_002438664.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain 1457c     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2217487..2228860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMUKE_RS10460 (ACMUKE_10460) cysE 2218497..2219126 (-) 630 WP_002438669.1 serine O-acetyltransferase -
  ACMUKE_RS10465 (ACMUKE_10465) gltX 2219511..2220965 (-) 1455 WP_002438667.1 glutamate--tRNA ligase -
  ACMUKE_RS10470 (ACMUKE_10470) - 2221375..2222433 (-) 1059 WP_001832313.1 PIN/TRAM domain-containing protein -
  ACMUKE_RS10475 (ACMUKE_10475) radA 2222487..2223860 (-) 1374 WP_002438664.1 DNA repair protein RadA Machinery gene
  ACMUKE_RS10480 (ACMUKE_10480) - 2224351..2226804 (-) 2454 WP_001832300.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMUKE_RS10485 (ACMUKE_10485) - 2226818..2227825 (-) 1008 WP_002438663.1 protein arginine kinase -
  ACMUKE_RS10490 (ACMUKE_10490) - 2227815..2228378 (-) 564 WP_002469112.1 UvrB/UvrC motif-containing protein -
  ACMUKE_RS10495 (ACMUKE_10495) - 2228399..2228860 (-) 462 WP_001832288.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50273.86 Da        Isoelectric Point: 7.5278

>NTDB_id=1094345 ACMUKE_RS10475 WP_002438664.1 2222487..2223860(-) (radA) [Staphylococcus epidermidis strain 1457c]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKQR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1094345 ACMUKE_RS10475 WP_002438664.1 2222487..2223860(-) (radA) [Staphylococcus epidermidis strain 1457c]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGCAAGTGCCCTAACTG
CGGTGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGTGTTAAGCATGAAGCTACCCCTAGATTACTTACAGAATCAAAGGAATTTAACCGTGTT
TTAGGTGGAGGTATTGTAAGTGGTTCGTTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCGACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTAGAAGAAGATTCTAGTCATCTTAATGTTTTAGCTGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTTGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCCGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACGCAGAGTTTAATGAACATTGCTAAACAAATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGACAAATCGCCGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGATGAG
CATCACGCATATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAAGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACAACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGCATTATTGTTGCGA
CAGCTTCAAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCTCGCATAGAGCAACGTGTTCAAGAAGCGGCCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAATAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTGAAATATGCATTACATT
CAAAACAGCGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

AAA domain (AAA_25)

(71-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.009

99.781

0.659
  radA Streptococcus pneumoniae Rx1

60.802

98.249

0.597
  radA Streptococcus pneumoniae D39

60.802

98.249

0.597
  radA Streptococcus pneumoniae R6

60.802

98.249

0.597
  radA Streptococcus pneumoniae TIGR4

60.802

98.249

0.597
  radA Streptococcus mitis NCTC 12261

60.802

98.249

0.597
  radA Streptococcus mitis SK321

60.802

98.249

0.597

Multiple sequence alignment