Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACMHYC_RS16335 Genome accession   NZ_CP180027
Coordinates   3455423..3456229 (-) Length   268 a.a.
NCBI ID   WP_032881569.1    Uniprot ID   -
Organism   Acinetobacter courvalinii isolate 2024CK-01798     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3450423..3461229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMHYC_RS16315 (ACMHYC_16315) - 3451480..3452178 (-) 699 WP_005226791.1 YfgM family protein -
  ACMHYC_RS16320 (ACMHYC_16320) hisS 3452208..3453500 (-) 1293 WP_414659443.1 histidine--tRNA ligase -
  ACMHYC_RS16325 (ACMHYC_16325) ispG 3453497..3454612 (-) 1116 WP_005226789.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACMHYC_RS16330 (ACMHYC_16330) - 3454635..3455432 (-) 798 WP_009513036.1 helix-turn-helix domain-containing protein -
  ACMHYC_RS16335 (ACMHYC_16335) pilF 3455423..3456229 (-) 807 WP_032881569.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACMHYC_RS16340 (ACMHYC_16340) rlmN 3456247..3457482 (-) 1236 WP_009513049.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACMHYC_RS16345 (ACMHYC_16345) ndk 3457604..3458035 (-) 432 WP_004775629.1 nucleoside-diphosphate kinase -
  ACMHYC_RS16350 (ACMHYC_16350) iscX 3458143..3458343 (-) 201 WP_005226785.1 Fe-S cluster assembly protein IscX -
  ACMHYC_RS16355 (ACMHYC_16355) - 3458451..3459002 (-) 552 WP_414659444.1 phosphatase PAP2 family protein -
  ACMHYC_RS16360 (ACMHYC_16360) - 3459035..3460306 (-) 1272 WP_032870212.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30401.37 Da        Isoelectric Point: 9.7102

>NTDB_id=1094100 ACMHYC_RS16335 WP_032881569.1 3455423..3456229(-) (pilF) [Acinetobacter courvalinii isolate 2024CK-01798]
MNTTRSKTIIATAVVFTLLGLTACQTPSTTLKKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKLNSRDANANMMMG
ILLQQEGSAANMEKAESYFKRAISSEPDNAQARNNYGTYLYQQQRYNEAIEQFTRAGATLGYDQRFQSLENLGRIYLKLG
DVANAEKAFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLV
NQLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=1094100 ACMHYC_RS16335 WP_032881569.1 3455423..3456229(-) (pilF) [Acinetobacter courvalinii isolate 2024CK-01798]
TTGAATACAACTCGATCAAAAACGATTATAGCGACAGCTGTTGTGTTTACTTTACTTGGCTTAACAGCTTGCCAGACGCC
ATCTACAACATTAAAAAAAGATCCGGAAAAAGCGGTGCAAGTGCGTACGCAACTTGCGGCAGAATATATTCGTACACGTG
ATCTGGATTCAGCCAAGCGTTCGCTTGATCAGGCATTAAAACTGAATTCCCGGGATGCCAACGCCAACATGATGATGGGC
ATCCTGCTGCAACAGGAAGGCAGCGCAGCCAATATGGAAAAAGCGGAGTCTTATTTTAAGCGTGCTATTTCCTCCGAACC
AGATAATGCTCAGGCGCGTAATAATTACGGTACATATTTGTACCAGCAGCAACGCTATAATGAAGCCATTGAGCAATTTA
CCCGTGCGGGCGCAACTCTGGGTTATGATCAGCGTTTTCAGTCGCTGGAAAACCTGGGGCGAATCTATTTAAAACTCGGC
GATGTGGCGAATGCTGAAAAAGCATTCAAACAGGCACTACAAGCGAATCGAAACGCAACAATTTCAATGTTAGAGTTGGC
GGAAATTTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTATACGAACAATATGTTCGTAATGTAGGTCAGAAAA
ACCAGGGTGCCCGTGCGCTTTGGATTGGTTTACGCATTGCTCGGGCAAATGCGGATCAGTTAGGAATGCAAGTGTTAGTC
AATCAGTTACGCGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATG
GAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(91-109)

Tetratricopeptide repeat (TPR_8)

(41-70)

Tetratricopeptide repeat (TPR_12)

(110-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.104

100

0.791
  pilF Acinetobacter baylyi ADP1

69.057

98.881

0.683

Multiple sequence alignment