Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   ACMHYC_RS02165 Genome accession   NZ_CP180027
Coordinates   462799..463230 (+) Length   143 a.a.
NCBI ID   WP_414659873.1    Uniprot ID   -
Organism   Acinetobacter courvalinii isolate 2024CK-01798     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 438420..471793 462799..463230 within 0
IScluster/Tn 463533..464665 462799..463230 flank 303


Gene organization within MGE regions


Location: 438420..471793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMHYC_RS02060 (ACMHYC_02060) - 439280..440986 (+) 1707 WP_414659605.1 ABC transporter ATP-binding protein -
  ACMHYC_RS02065 (ACMHYC_02065) - 441001..441726 (+) 726 WP_414659606.1 class I SAM-dependent methyltransferase -
  ACMHYC_RS02070 (ACMHYC_02070) - 441719..443032 (+) 1314 WP_414659607.1 glycosyltransferase family 4 protein -
  ACMHYC_RS02075 (ACMHYC_02075) - 443029..444039 (-) 1011 WP_414659608.1 glycosyltransferase family 4 protein -
  ACMHYC_RS02080 (ACMHYC_02080) - 444139..445563 (+) 1425 WP_414659609.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  ACMHYC_RS02085 (ACMHYC_02085) - 445560..446696 (-) 1137 WP_414659610.1 glycosyltransferase family 4 protein -
  ACMHYC_RS02090 (ACMHYC_02090) - 446916..447371 (+) 456 WP_414659611.1 pilin -
  ACMHYC_RS02095 (ACMHYC_02095) - 447754..449406 (+) 1653 WP_414659612.1 Wzy polymerase domain-containing protein -
  ACMHYC_RS02100 (ACMHYC_02100) bfr 449468..449932 (-) 465 WP_005227625.1 heteropolymeric bacterioferritin subunit Bfr -
  ACMHYC_RS02105 (ACMHYC_02105) - 450199..450393 (-) 195 WP_004776027.1 bacterioferritin-associated ferredoxin -
  ACMHYC_RS02110 (ACMHYC_02110) - 450575..450955 (-) 381 WP_005227623.1 RidA family protein -
  ACMHYC_RS02115 (ACMHYC_02115) - 451120..453234 (-) 2115 WP_396514401.1 RelA/SpoT family protein -
  ACMHYC_RS02120 (ACMHYC_02120) rpoZ 453442..453720 (-) 279 WP_004657467.1 DNA-directed RNA polymerase subunit omega -
  ACMHYC_RS02125 (ACMHYC_02125) gmk 453790..454413 (-) 624 WP_005227618.1 guanylate kinase -
  ACMHYC_RS02130 (ACMHYC_02130) ispH 454517..455467 (+) 951 WP_032873671.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACMHYC_RS02135 (ACMHYC_02135) - 455608..456054 (+) 447 WP_414659613.1 Tfp pilus assembly protein FimT/FimU -
  ACMHYC_RS02140 (ACMHYC_02140) pilV 456064..456585 (+) 522 WP_414659614.1 type IV pilus modification protein PilV -
  ACMHYC_RS02145 (ACMHYC_02145) - 456578..457576 (+) 999 WP_414659871.1 PilW family protein -
  ACMHYC_RS02150 (ACMHYC_02150) - 457576..458289 (+) 714 WP_414659872.1 pilus assembly protein PilX -
  ACMHYC_RS02155 (ACMHYC_02155) - 458318..462187 (+) 3870 WP_414659615.1 pilus assembly protein PilY -
  ACMHYC_RS02160 (ACMHYC_02160) - 462197..462793 (+) 597 WP_414659616.1 type IV pilin protein -
  ACMHYC_RS02165 (ACMHYC_02165) comF 462799..463230 (+) 432 WP_414659873.1 type IV pilin protein Machinery gene
  ACMHYC_RS02175 (ACMHYC_02175) - 464831..465334 (+) 504 WP_414659617.1 GspH/FimT family pseudopilin -
  ACMHYC_RS02180 (ACMHYC_02180) pilV 465331..465816 (+) 486 WP_414659618.1 type IV pilus modification protein PilV Machinery gene
  ACMHYC_RS02185 (ACMHYC_02185) - 465818..466846 (+) 1029 WP_414659619.1 PilW family protein -
  ACMHYC_RS02190 (ACMHYC_02190) pilX 466843..467574 (+) 732 WP_414659620.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACMHYC_RS02195 (ACMHYC_02195) - 467588..471793 (+) 4206 WP_414659621.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15779.92 Da        Isoelectric Point: 8.9468

>NTDB_id=1094080 ACMHYC_RS02165 WP_414659873.1 462799..463230(+) (comF) [Acinetobacter courvalinii isolate 2024CK-01798]
MVERGFTLIELMIVVAIIAILAAIAYPSYQEYVKRTKRADLQSEMMNIAQRLQSYRVINHKYTNAKLDNGTLTKNYPASN
ASYTIDLTTADQTWVLTATPINGQIGDGHVVLNNQGHKCWTKGSDKNGGTACVPSATSNWDGR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1094080 ACMHYC_RS02165 WP_414659873.1 462799..463230(+) (comF) [Acinetobacter courvalinii isolate 2024CK-01798]
ATGGTTGAACGAGGTTTTACTTTAATTGAGCTCATGATTGTAGTCGCAATTATTGCTATTTTGGCTGCTATAGCTTATCC
CTCTTATCAAGAGTATGTAAAACGTACTAAACGTGCTGATCTACAGAGTGAAATGATGAATATCGCACAACGACTGCAAA
GTTATCGTGTGATTAATCATAAATATACAAATGCAAAATTGGATAATGGAACATTAACAAAAAATTATCCAGCGTCTAAT
GCATCTTATACTATTGATTTAACTACTGCTGATCAAACTTGGGTACTTACAGCGACTCCTATTAATGGACAAATAGGTGA
TGGACATGTGGTTTTAAATAATCAAGGTCATAAATGCTGGACCAAAGGTTCTGATAAGAATGGTGGTACGGCATGTGTTC
CATCCGCAACCAGCAACTGGGATGGTCGGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(27-107)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

51.701

100

0.531
  pilE Acinetobacter baumannii D1279779

51.007

100

0.531
  comE Acinetobacter baylyi ADP1

36.025

100

0.406

Multiple sequence alignment