Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMG4L_RS15355 Genome accession   NZ_CP179933
Coordinates   3267696..3269063 (+) Length   455 a.a.
NCBI ID   WP_035245000.1    Uniprot ID   -
Organism   Alcanivorax sp. IL1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3262696..3274063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4L_RS15325 (ACMG4L_15325) rpsF 3263291..3263632 (+) 342 WP_035245010.1 30S ribosomal protein S6 -
  ACMG4L_RS15330 (ACMG4L_15330) priB 3263686..3263949 (+) 264 WP_414433546.1 primosomal replication protein N -
  ACMG4L_RS15335 (ACMG4L_15335) rpsR 3263965..3264195 (+) 231 WP_035245006.1 30S ribosomal protein S18 -
  ACMG4L_RS15340 (ACMG4L_15340) rplI 3264211..3264663 (+) 453 WP_035245005.1 50S ribosomal protein L9 -
  ACMG4L_RS15345 (ACMG4L_15345) dnaB 3264924..3266317 (+) 1394 Protein_3019 replicative DNA helicase -
  ACMG4L_RS15350 (ACMG4L_15350) alr 3266403..3267464 (+) 1062 WP_035245001.1 alanine racemase -
  ACMG4L_RS15355 (ACMG4L_15355) radA 3267696..3269063 (+) 1368 WP_035245000.1 DNA repair protein RadA Machinery gene
  ACMG4L_RS15360 (ACMG4L_15360) - 3269231..3269662 (-) 432 WP_273009844.1 hypothetical protein -
  ACMG4L_RS15365 (ACMG4L_15365) - 3269666..3270460 (-) 795 WP_035244997.1 DUF547 domain-containing protein -
  ACMG4L_RS15370 (ACMG4L_15370) lpdA 3270539..3272683 (-) 2145 WP_035244996.1 dihydrolipoyl dehydrogenase -
  ACMG4L_RS15375 (ACMG4L_15375) - 3272702..3272839 (-) 138 WP_156964813.1 hypothetical protein -
  ACMG4L_RS15380 (ACMG4L_15380) - 3272934..3273977 (-) 1044 WP_414430293.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48546.29 Da        Isoelectric Point: 7.3476

>NTDB_id=1093992 ACMG4L_RS15355 WP_035245000.1 3267696..3269063(+) (radA) [Alcanivorax sp. IL1]
MAKKKTAFVCTDCGADFPKWQGQCPACGAWNTLQEFVHDPATPMSKGAGSRGGFSGQLSEVQNLGEIVLAETPRISSSMG
ELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQMLCKLAATQKCLYITGEESLSQVAMRADRLKLPKEQLRLAAETDVEQI
LDLVRKEQPRVLVVDSIQVMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCSLM
LEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRADDIASGSLVTVVWEGTRPLLVELQALVDASH
FGNPRRVCVGLEANRLAMLLAVLHRHGGIQVGDQDVFMNVVGGVKVTETAADLAQVLAIVSSFRDRALGRELVVFGEVGL
SGEIRPVPQGQERLYEAVKHGFKKAIVPKANLPRQLPEGVEVIGVSTVGEALEYL

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1093992 ACMG4L_RS15355 WP_035245000.1 3267696..3269063(+) (radA) [Alcanivorax sp. IL1]
GTGGCCAAGAAAAAAACTGCCTTTGTCTGCACCGATTGTGGCGCTGATTTCCCCAAGTGGCAGGGGCAATGCCCCGCCTG
TGGCGCCTGGAACACCCTGCAGGAATTCGTTCATGATCCGGCCACGCCCATGTCCAAGGGCGCAGGTAGCCGTGGCGGCT
TTTCCGGGCAATTGTCCGAGGTGCAGAACCTTGGCGAGATCGTGCTGGCGGAAACCCCGCGCATCAGTTCCAGCATGGGC
GAGCTGGACCGGGTGCTGGGCGGAGGCCTGGTGCCGGGCTCGGCGATCCTGATTGGCGGGCACCCCGGTGCCGGCAAATC
CACCTTGTTGCTGCAAATGCTGTGCAAGCTGGCGGCCACCCAGAAGTGCCTTTATATCACCGGTGAAGAATCCCTCTCCC
AGGTGGCCATGCGCGCTGACCGGCTCAAGCTGCCCAAGGAGCAGCTGCGGCTGGCGGCGGAAACCGATGTGGAGCAAATA
CTGGATCTGGTTCGCAAGGAGCAGCCCCGGGTGCTGGTGGTCGATTCCATTCAGGTCATGTTCCTTGCTGCCCTGCAGTC
CGCCCCGGGCAGTGTGGCCCAGGTGCGTGAGTGTGCGGCGGCGTTGACCCGGTTTGCCAAGCAGACCGGCACCGTGCTGT
TGCTGGTGGGCCATGTCACCAAGGATGGCACCCTGGCTGGCCCCAAGGTGCTGGAGCATATGATCGACTGCTCACTAATG
CTGGAAGGCTCCACGGATTCACGTTTTCGCACCTTACGTGGGCTGAAAAACCGTTTCGGTGCAGTCAACGAGCTAGGTGT
GTTTGCCATGACCGGCGAAGGCCTGAAAGAAGTGAAGAACCCCTCAGCCATCTTCCTCAACCGGGCCGACGACATTGCGT
CCGGTTCCCTGGTGACGGTGGTCTGGGAGGGTACTCGTCCCTTGCTGGTAGAGCTGCAGGCGCTGGTGGATGCCTCCCAC
TTCGGCAACCCCCGCCGGGTCTGTGTGGGCCTGGAGGCAAATCGTCTGGCCATGCTGCTGGCGGTGCTGCATCGCCATGG
TGGCATCCAGGTAGGCGATCAGGATGTGTTCATGAATGTGGTGGGTGGCGTCAAGGTCACCGAAACCGCCGCCGACCTGG
CCCAGGTACTGGCCATCGTCTCCAGCTTTCGCGACCGGGCTCTGGGCCGGGAACTGGTGGTGTTCGGCGAAGTGGGCCTG
TCCGGCGAAATCCGCCCGGTGCCCCAGGGCCAGGAGCGTCTCTACGAAGCGGTCAAACACGGCTTCAAGAAAGCCATTGT
TCCCAAGGCAAACCTTCCACGACAACTGCCGGAAGGGGTGGAGGTGATCGGGGTGAGTACGGTGGGGGAGGCGCTGGAAT
ACCTTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(347-431)

AAA domain (AAA_25)

(79-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.796

100

0.49
  radA Streptococcus pneumoniae Rx1

44.934

99.78

0.448
  radA Streptococcus pneumoniae D39

44.934

99.78

0.448
  radA Streptococcus pneumoniae R6

44.934

99.78

0.448
  radA Streptococcus pneumoniae TIGR4

44.934

99.78

0.448
  radA Streptococcus mitis NCTC 12261

44.714

99.78

0.446
  radA Streptococcus mitis SK321

44.714

99.78

0.446

Multiple sequence alignment