Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMG4N_RS05620 Genome accession   NZ_CP179932
Coordinates   1206476..1207843 (+) Length   455 a.a.
NCBI ID   WP_297769832.1    Uniprot ID   -
Organism   Alcanivorax sp. IL2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1201476..1212843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4N_RS05590 (ACMG4N_05590) rpsF 1202064..1202405 (+) 342 WP_035245010.1 30S ribosomal protein S6 -
  ACMG4N_RS05595 (ACMG4N_05595) priB 1202408..1202722 (+) 315 WP_297769819.1 primosomal replication protein N -
  ACMG4N_RS05600 (ACMG4N_05600) rpsR 1202738..1202968 (+) 231 WP_035245006.1 30S ribosomal protein S18 -
  ACMG4N_RS05605 (ACMG4N_05605) rplI 1202984..1203436 (+) 453 WP_297769824.1 50S ribosomal protein L9 -
  ACMG4N_RS05610 (ACMG4N_05610) dnaB 1203698..1205092 (+) 1395 WP_035245003.1 replicative DNA helicase -
  ACMG4N_RS05615 (ACMG4N_05615) alr 1205183..1206244 (+) 1062 WP_414433066.1 alanine racemase -
  ACMG4N_RS05620 (ACMG4N_05620) radA 1206476..1207843 (+) 1368 WP_297769832.1 DNA repair protein RadA Machinery gene
  ACMG4N_RS05625 (ACMG4N_05625) - 1207994..1208434 (-) 441 WP_414433067.1 hypothetical protein -
  ACMG4N_RS05630 (ACMG4N_05630) - 1208438..1209232 (-) 795 WP_414433068.1 DUF547 domain-containing protein -
  ACMG4N_RS05635 (ACMG4N_05635) - 1209309..1211453 (-) 2145 WP_414433069.1 FAD-dependent oxidoreductase -
  ACMG4N_RS05640 (ACMG4N_05640) - 1211611..1212654 (-) 1044 WP_414433070.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48574.28 Da        Isoelectric Point: 7.3477

>NTDB_id=1093953 ACMG4N_RS05620 WP_297769832.1 1206476..1207843(+) (radA) [Alcanivorax sp. IL2]
MAKKKTAFVCTDCGADFPKWQGQCPACGTWNTLQEFVHDPATPMSKGAGSRGGFSGQLSEVQNLGEIVLAETPRISSSMG
ELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQTLCKLATVQKCLYITGEESLSQVAMRADRLKLPKEQLRLAAETDVEQI
LDLARKEQPRVLVVDSIQVMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCSLM
LEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRADEIASGSLVTVVWEGTRPLLVELQALVDASH
FGNPRRVCVGLEANRLAMLLAVLHRHGGIQVGDQDVFMNVVGGVKVTETAADLAQVLAIVSSFRDRALGRELVVFGEVGL
SGEIRPVPQGQERLYEAVKHGFKKAIVPKANLPRQLPEGVEVIGVSTVAEALEYL

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1093953 ACMG4N_RS05620 WP_297769832.1 1206476..1207843(+) (radA) [Alcanivorax sp. IL2]
GTGGCCAAGAAAAAAACTGCCTTTGTGTGCACCGATTGTGGCGCTGATTTCCCCAAGTGGCAGGGGCAATGCCCCGCCTG
TGGCACCTGGAATACCCTGCAGGAATTCGTCCATGACCCGGCTACCCCCATGTCGAAAGGTGCCGGTAGCCGTGGCGGTT
TTTCCGGGCAATTGTCCGAGGTGCAGAATCTGGGTGAGATCGTGCTGGCGGAAACGCCACGCATCAGTTCCAGCATGGGC
GAGCTGGACCGGGTGCTGGGCGGTGGTCTGGTGCCGGGCTCGGCGATCCTGATTGGCGGGCACCCCGGTGCCGGCAAATC
TACCCTGTTGCTGCAAACCTTGTGCAAGCTGGCGACTGTCCAGAAGTGCCTTTATATCACCGGTGAAGAGTCCCTTTCCC
AGGTGGCCATGCGCGCTGACCGGCTCAAGCTGCCCAAGGAACAGCTGCGGCTGGCGGCGGAAACCGATGTGGAACAGATC
TTGGATCTGGCTCGCAAGGAACAGCCCCGGGTGCTGGTGGTCGATTCCATTCAGGTCATGTTCCTTGCTGCCCTGCAGTC
CGCCCCGGGCAGTGTGGCCCAGGTGCGTGAGTGTGCGGCGGCGTTGACCCGTTTTGCCAAACAGACCGGCACCGTGCTGT
TGCTTGTGGGCCATGTCACCAAGGATGGCACCCTGGCTGGTCCCAAGGTTCTCGAGCATATGATCGACTGCTCGCTGATG
CTGGAAGGCTCCACGGATTCACGGTTCCGCACCTTGCGCGGGCTGAAAAACCGTTTCGGCGCAGTGAACGAGCTGGGCGT
ATTCGCCATGACCGGCGAAGGGCTGAAAGAAGTGAAGAACCCCTCTGCCATCTTTCTCAACCGGGCCGATGAGATTGCGT
CCGGTTCCCTGGTAACGGTGGTCTGGGAAGGTACCCGACCCTTGCTGGTGGAACTGCAGGCGCTGGTGGATGCCTCCCAC
TTCGGCAACCCGCGCCGGGTTTGTGTGGGCCTGGAGGCCAATCGTCTGGCCATGTTGTTGGCGGTGCTGCATCGCCATGG
CGGCATCCAGGTGGGCGATCAGGATGTGTTCATGAATGTGGTGGGAGGCGTCAAAGTTACCGAAACCGCCGCCGACCTGG
CCCAGGTGCTGGCCATCGTCTCCAGCTTCCGCGATCGGGCCCTTGGGCGTGAGCTGGTGGTGTTCGGCGAAGTGGGCCTG
TCCGGCGAAATCCGCCCGGTGCCCCAGGGCCAGGAGCGTCTTTACGAAGCGGTCAAACACGGCTTCAAGAAAGCCATTGT
TCCCAAGGCAAACTTGCCGCGCCAGCTGCCGGAAGGGGTGGAGGTGATCGGGGTGAGTACGGTGGCGGAGGCGCTGGAAT
ACTTGTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(79-223)

Subunit ChlI of Mg-chelatase (ChlI)

(347-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.796

100

0.49
  radA Streptococcus pneumoniae Rx1

44.714

99.78

0.446
  radA Streptococcus pneumoniae D39

44.714

99.78

0.446
  radA Streptococcus pneumoniae R6

44.714

99.78

0.446
  radA Streptococcus pneumoniae TIGR4

44.714

99.78

0.446
  radA Streptococcus mitis NCTC 12261

44.493

99.78

0.444
  radA Streptococcus mitis SK321

44.493

99.78

0.444

Multiple sequence alignment