Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMG4M_RS14680 Genome accession   NZ_CP179931
Coordinates   3149477..3150844 (-) Length   455 a.a.
NCBI ID   WP_035245000.1    Uniprot ID   -
Organism   Alcanivorax sp. IL3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3144477..3155844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4M_RS14655 (ACMG4M_14655) - 3144563..3145606 (+) 1044 WP_414430293.1 methyltransferase domain-containing protein -
  ACMG4M_RS14660 (ACMG4M_14660) - 3145701..3145838 (+) 138 WP_156964813.1 hypothetical protein -
  ACMG4M_RS14665 (ACMG4M_14665) lpdA 3145857..3148001 (+) 2145 WP_035244996.1 dihydrolipoyl dehydrogenase -
  ACMG4M_RS14670 (ACMG4M_14670) - 3148080..3148874 (+) 795 WP_035244997.1 DUF547 domain-containing protein -
  ACMG4M_RS14675 (ACMG4M_14675) - 3148878..3149309 (+) 432 WP_273009844.1 hypothetical protein -
  ACMG4M_RS14680 (ACMG4M_14680) radA 3149477..3150844 (-) 1368 WP_035245000.1 DNA repair protein RadA Machinery gene
  ACMG4M_RS14685 (ACMG4M_14685) alr 3151076..3152137 (-) 1062 WP_035245001.1 alanine racemase -
  ACMG4M_RS14690 (ACMG4M_14690) dnaB 3152223..3153617 (-) 1395 WP_035245003.1 replicative DNA helicase -
  ACMG4M_RS14695 (ACMG4M_14695) rplI 3153878..3154330 (-) 453 WP_035245005.1 50S ribosomal protein L9 -
  ACMG4M_RS14700 (ACMG4M_14700) rpsR 3154346..3154576 (-) 231 WP_035245006.1 30S ribosomal protein S18 -
  ACMG4M_RS14705 (ACMG4M_14705) priB 3154592..3154906 (-) 315 WP_297769819.1 primosomal replication protein N -
  ACMG4M_RS14710 (ACMG4M_14710) rpsF 3154909..3155250 (-) 342 WP_035245010.1 30S ribosomal protein S6 -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48546.29 Da        Isoelectric Point: 7.3476

>NTDB_id=1093942 ACMG4M_RS14680 WP_035245000.1 3149477..3150844(-) (radA) [Alcanivorax sp. IL3]
MAKKKTAFVCTDCGADFPKWQGQCPACGAWNTLQEFVHDPATPMSKGAGSRGGFSGQLSEVQNLGEIVLAETPRISSSMG
ELDRVLGGGLVPGSAILIGGHPGAGKSTLLLQMLCKLAATQKCLYITGEESLSQVAMRADRLKLPKEQLRLAAETDVEQI
LDLVRKEQPRVLVVDSIQVMFLAALQSAPGSVAQVRECAAALTRFAKQTGTVLLLVGHVTKDGTLAGPKVLEHMIDCSLM
LEGSTDSRFRTLRGLKNRFGAVNELGVFAMTGEGLKEVKNPSAIFLNRADDIASGSLVTVVWEGTRPLLVELQALVDASH
FGNPRRVCVGLEANRLAMLLAVLHRHGGIQVGDQDVFMNVVGGVKVTETAADLAQVLAIVSSFRDRALGRELVVFGEVGL
SGEIRPVPQGQERLYEAVKHGFKKAIVPKANLPRQLPEGVEVIGVSTVGEALEYL

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1093942 ACMG4M_RS14680 WP_035245000.1 3149477..3150844(-) (radA) [Alcanivorax sp. IL3]
GTGGCCAAGAAAAAAACTGCCTTTGTCTGCACCGATTGTGGCGCTGATTTCCCCAAGTGGCAGGGGCAATGCCCCGCCTG
TGGCGCCTGGAACACCCTGCAGGAATTCGTTCATGATCCGGCCACGCCCATGTCCAAGGGCGCAGGTAGCCGTGGCGGCT
TTTCCGGGCAATTGTCCGAGGTGCAGAACCTTGGCGAGATCGTGCTGGCGGAAACCCCGCGCATCAGTTCCAGCATGGGC
GAGCTGGACCGGGTGCTGGGCGGAGGCCTGGTGCCGGGCTCGGCGATCCTGATTGGCGGGCACCCCGGTGCCGGCAAATC
CACCTTGTTGCTGCAAATGCTGTGCAAGCTGGCGGCCACCCAGAAGTGCCTTTATATCACCGGTGAAGAATCCCTCTCCC
AGGTGGCCATGCGCGCTGACCGGCTCAAGCTGCCCAAGGAGCAGCTGCGGCTGGCGGCGGAAACCGATGTGGAGCAAATA
CTGGATCTGGTTCGCAAGGAGCAGCCCCGGGTGCTGGTGGTCGATTCCATTCAGGTCATGTTCCTTGCTGCCCTGCAGTC
CGCCCCGGGCAGTGTGGCCCAGGTGCGTGAGTGTGCGGCGGCGTTGACCCGGTTTGCCAAGCAGACCGGCACCGTGCTGT
TGCTGGTGGGCCATGTCACCAAGGATGGCACCCTGGCTGGCCCCAAGGTGCTGGAGCATATGATCGACTGCTCACTAATG
CTGGAAGGCTCCACGGATTCACGTTTTCGCACCTTACGTGGGCTGAAAAACCGTTTCGGTGCAGTCAACGAGCTAGGTGT
GTTTGCCATGACCGGCGAAGGCCTGAAAGAAGTGAAGAACCCCTCAGCCATCTTCCTCAACCGGGCCGACGACATTGCGT
CCGGTTCCCTGGTGACGGTGGTCTGGGAGGGTACTCGTCCCTTGCTGGTAGAGCTGCAGGCGCTGGTGGATGCCTCCCAC
TTCGGCAACCCCCGCCGGGTCTGTGTGGGCCTGGAGGCAAATCGTCTGGCCATGCTGCTGGCGGTGCTGCATCGCCATGG
TGGCATCCAGGTAGGCGATCAGGATGTGTTCATGAATGTGGTGGGTGGCGTCAAGGTCACCGAAACCGCCGCCGACCTGG
CCCAGGTACTGGCCATCGTCTCCAGCTTTCGCGACCGGGCTCTGGGCCGGGAACTGGTGGTGTTCGGCGAAGTGGGCCTG
TCCGGCGAAATCCGCCCGGTGCCCCAGGGCCAGGAGCGTCTCTACGAAGCGGTCAAACACGGCTTCAAGAAAGCCATTGT
TCCCAAGGCAAACCTTCCACGACAACTGCCGGAAGGGGTGGAGGTGATCGGGGTGAGTACGGTGGGGGAGGCGCTGGAAT
ACCTTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(347-431)

AAA domain (AAA_25)

(79-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.796

100

0.49
  radA Streptococcus pneumoniae Rx1

44.934

99.78

0.448
  radA Streptococcus pneumoniae D39

44.934

99.78

0.448
  radA Streptococcus pneumoniae R6

44.934

99.78

0.448
  radA Streptococcus pneumoniae TIGR4

44.934

99.78

0.448
  radA Streptococcus mitis NCTC 12261

44.714

99.78

0.446
  radA Streptococcus mitis SK321

44.714

99.78

0.446

Multiple sequence alignment