Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACJ3UO_RS00285 Genome accession   NZ_CP179927
Coordinates   54334..55722 (+) Length   462 a.a.
NCBI ID   WP_010935866.1    Uniprot ID   Q3ZAD8
Organism   MAG: Dehalococcoides mccartyi isolate NK     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 49334..60722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3UO_RS00280 (ACJ3UO_00295) - 51640..54114 (+) 2475 WP_010935865.1 ATP-dependent Clp protease ATP-binding subunit -
  ACJ3UO_RS00285 (ACJ3UO_00300) radA 54334..55722 (+) 1389 WP_010935866.1 DNA repair protein RadA Machinery gene
  ACJ3UO_RS00290 (ACJ3UO_00310) - 55709..56392 (+) 684 WP_010935867.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACJ3UO_RS00295 (ACJ3UO_00315) ispF 56398..56871 (+) 474 WP_010935868.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACJ3UO_RS00300 (ACJ3UO_00320) cysS 56875..58251 (+) 1377 WP_010935869.1 cysteine--tRNA ligase -
  ACJ3UO_RS00310 (ACJ3UO_00330) - 58467..59465 (+) 999 WP_305757375.1 tyrosine-type recombinase/integrase -
  ACJ3UO_RS00315 (ACJ3UO_00335) - 59492..60358 (-) 867 WP_011928582.1 Bro-N domain-containing protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 49545.99 Da        Isoelectric Point: 7.6049

>NTDB_id=1093881 ACJ3UO_RS00285 WP_010935866.1 54334..55722(+) (radA) [MAG: Dehalococcoides mccartyi isolate NK]
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVVSPIGRKNEPARVISPAAELSSLQASETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPLLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=1093881 ACJ3UO_RS00285 WP_010935866.1 54334..55722(+) (radA) [MAG: Dehalococcoides mccartyi isolate NK]
ATGGAAAAAAGCCGTAGAGTTTATGTTTGTTCCAACTGCGGGCGTGAGAGCCTGAAATGGCTGGGCCGCTGCCCCGCCTG
TCAGGAGTGGAATACTTTTGAAGAAAAAACGGTTGTTTCGCCCATAGGGCGTAAAAATGAACCTGCCCGGGTTATCAGCC
CGGCGGCAGAGCTTTCCAGTTTACAGGCATCCGAAACCACCCGGCGGAGTTTATCCATTTCGGAGTTTAACCGGGTTTTA
GGCGGCGGCATAGTGCCGGGTTCGCTTATGCTTTTAGGGGGTGAACCGGGCATCGGCAAATCCACTCTGCTTCTTCAGGT
GGCGGCTTCGGTAGCCCAAAGCGGCGGCAAGGTAGTATATGTATCCGGGGAAGAAAACCCCGCCCAGATAAAAATGCGCG
CCCAACGCCTGGGTATCAGCGGCGAAGGGCTTTTTCTTATGGCTGAGACTGACCTTAATGCCATTTTAGCCCAGCTTTCA
GTCCTCTGCCCGTTACTGGTCGTTATTGACTCTATCCAGACTGTATTTCTGCCGGAGCTTGAAGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAGTCAGCCCTCCGCCTGATGCAGTGGGCTAAAAGCAGCGGAGCCAGCGTATTTATAACCGCCCATG
TTACCAAAGAGGGAAACATAGCCGGGCCGCGTATACTGGAACATATAGTAGATGTGGTTATGTACTTTGAGGGAGAGTCC
CAGAGTGCTTACCGTTTAGTGCGTTCGGTAAAAAACCGTTTCGGTTCTACCAACGAAGTGGGTATATTTGAAATGAAAAG
TGAGGGTCTGGTAGAAGTAGCCAACCCTTCCCAGATATTTTTATCAAACCGCCAGGCAAATACGGTCGGCTCGGCTGTAA
CCGCTGTGCTGGAGGGCAGCCGCCCGCTGCTGGTTGAAGTGCAGGCACTTACCAATACCACCAGTTTCGGCCAGCCCCGC
CGCACCGCCAACGGGGTGGATTTTAACCGTACCCTTATGATAGCCGCTGTCCTCTCCAAACGCCTGTCCATGCGTTTAGG
TACCCAGGACATAATAGTCAACGCTACCGGCGGTATCCGTCTGGATGAGCCGGCCGCAGATTTAGCTATAGCTCTGGCCA
TTGCTTCCAGTTACCGGGATACCGGCGTCTGCCCAGAAACCATTGCTCTTGGGGAGATTGGTCTTTCTGGTGAACTGCGG
ACTATTCCCCATCTGGAAAGGCGTCTTTCCGAGGCCAGCCGTCTGGGTTTTAAGAGGGCTTTAGTACCGGCCGGTGCTAA
CTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCGGTGAAGCTGGCACTTACCGGGGTAA
AAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(62-218)

Subunit ChlI of Mg-chelatase (ChlI)

(342-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3ZAD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.891

99.351

0.496
  radA Streptococcus pneumoniae Rx1

48.578

98.918

0.481
  radA Streptococcus pneumoniae D39

48.578

98.918

0.481
  radA Streptococcus pneumoniae R6

48.578

98.918

0.481
  radA Streptococcus pneumoniae TIGR4

48.578

98.918

0.481
  radA Streptococcus mitis SK321

48.578

98.918

0.481
  radA Streptococcus mitis NCTC 12261

48.359

98.918

0.478

Multiple sequence alignment