Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACGHXM_RS07840 Genome accession   NZ_AP031433
Coordinates   1666410..1667354 (-) Length   314 a.a.
NCBI ID   WP_014017056.1    Uniprot ID   G0VLY9
Organism   Megasphaera elsdenii strain i33-0019-1i4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1661410..1672354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHXM_RS07815 (I3300191I4_15290) - 1662712..1663884 (-) 1173 WP_054336366.1 efflux RND transporter periplasmic adaptor subunit -
  ACGHXM_RS07820 (I3300191I4_15300) - 1663887..1664477 (-) 591 WP_022496855.1 TetR/AcrR family transcriptional regulator -
  ACGHXM_RS07825 (I3300191I4_15310) thiW 1664611..1665114 (-) 504 WP_014017053.1 energy coupling factor transporter S component ThiW -
  ACGHXM_RS07830 (I3300191I4_15320) thiM 1665107..1665919 (-) 813 WP_390608692.1 hydroxyethylthiazole kinase -
  ACGHXM_RS07835 (I3300191I4_15330) - 1665934..1666398 (-) 465 WP_014017055.1 YhcH/YjgK/YiaL family protein -
  ACGHXM_RS07840 (I3300191I4_15340) pilA 1666410..1667354 (-) 945 WP_014017056.1 signal recognition particle-docking protein FtsY Machinery gene
  ACGHXM_RS07845 (I3300191I4_15350) smc 1667357..1670911 (-) 3555 WP_390608694.1 chromosome segregation protein SMC -
  ACGHXM_RS07850 (I3300191I4_15360) - 1671158..1672111 (+) 954 WP_014017058.1 magnesium transporter CorA family protein -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 33780.64 Da        Isoelectric Point: 4.7563

>NTDB_id=109365 ACGHXM_RS07840 WP_014017056.1 1666410..1667354(-) (pilA) [Megasphaera elsdenii strain i33-0019-1i4]
MGFFSKLRKGLEKTKHSLIQNIETVVRGYAKIDDDMYDDLEAVMLTGDIGVETTEYLLDQIRTGVKSKEIKDGNDVVPYL
EKCIVALLNENDEPLPETEGPRVIFIVGVNGVGKTTTIGKLAKYYKQQGKSVMLAAGDTFRAAASEQLTIWADRVGVPIV
KHQEGADAAAVVFDATASAKAKGMDILLVDTAGRLQTKSNLMEELRKMARVAGRNIEGAPQETLLVLDATTGQNAVSQAK
LFGDVVPLTGVVLTKLDGTAKGGVILSVKRELGVPVRWVGVGEGVDDLRPFDAAQFADALFDKNVAAREASDED

Nucleotide


Download         Length: 945 bp        

>NTDB_id=109365 ACGHXM_RS07840 WP_014017056.1 1666410..1667354(-) (pilA) [Megasphaera elsdenii strain i33-0019-1i4]
ATGGGATTTTTTTCAAAACTTCGAAAAGGCTTAGAAAAGACGAAACATTCGCTCATCCAGAACATCGAAACCGTCGTCCG
CGGCTATGCCAAAATAGACGACGACATGTACGATGACCTGGAAGCGGTCATGCTCACGGGAGACATCGGCGTCGAAACGA
CGGAGTATCTCCTGGACCAGATCCGGACCGGCGTCAAATCGAAGGAAATCAAAGACGGCAACGACGTCGTACCGTATTTG
GAAAAATGCATCGTCGCCTTGCTCAATGAAAACGATGAACCCCTCCCGGAAACGGAAGGGCCGCGGGTCATCTTCATCGT
CGGCGTCAATGGTGTCGGTAAGACGACGACTATCGGCAAACTGGCCAAGTATTACAAACAGCAGGGGAAATCGGTCATGC
TCGCCGCCGGCGATACCTTCCGCGCTGCTGCCTCGGAACAGCTGACCATCTGGGCTGACCGCGTCGGCGTACCCATCGTC
AAGCACCAGGAAGGGGCGGACGCAGCGGCTGTCGTCTTTGATGCGACGGCGTCGGCCAAGGCCAAGGGCATGGATATCCT
CCTTGTCGATACGGCCGGCCGCCTGCAGACCAAGTCCAACCTCATGGAAGAATTGCGGAAAATGGCCCGCGTCGCCGGCC
GCAACATCGAAGGGGCGCCGCAGGAAACGCTCCTCGTCCTCGATGCCACGACGGGGCAGAACGCTGTCAGCCAGGCCAAG
CTCTTCGGCGACGTCGTTCCCTTGACGGGCGTCGTCCTGACCAAGCTCGACGGAACAGCCAAAGGCGGCGTCATCTTGTC
GGTGAAACGGGAATTAGGCGTCCCTGTCCGCTGGGTCGGCGTCGGCGAAGGCGTCGACGACCTGCGCCCCTTCGATGCAG
CTCAGTTCGCCGATGCTCTGTTCGATAAAAACGTCGCCGCCCGCGAAGCCAGCGACGAAGATTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(21-80)

SRP54-type protein, GTPase domain (SRP54)

(101-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0VLY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.645

96.815

0.5

Multiple sequence alignment