Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACK8DD_RS15975 Genome accession   NZ_CP179880
Coordinates   3384249..3385049 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN548254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379249..3390049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DD_RS15955 (ACK8DD_15955) - 3380314..3381012 (-) 699 WP_000058788.1 YfgM family protein -
  ACK8DD_RS15960 (ACK8DD_15960) hisS 3381041..3382333 (-) 1293 WP_000095253.1 histidine--tRNA ligase -
  ACK8DD_RS15965 (ACK8DD_15965) ispG 3382330..3383445 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACK8DD_RS15970 (ACK8DD_15970) - 3383467..3384258 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  ACK8DD_RS15975 (ACK8DD_15975) pilF 3384249..3385049 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACK8DD_RS15980 (ACK8DD_15980) rlmN 3385065..3386297 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACK8DD_RS15985 (ACK8DD_15985) ndk 3386420..3386851 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ACK8DD_RS15990 (ACK8DD_15990) iscX 3386966..3387166 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  ACK8DD_RS15995 (ACK8DD_15995) - 3387266..3387816 (-) 551 Protein_3125 phosphatase PAP2 family protein -
  ACK8DD_RS16000 (ACK8DD_16000) - 3387841..3389139 (-) 1299 WP_140982977.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=1093360 ACK8DD_RS15975 WP_004738086.1 3384249..3385049(-) (pilF) [Acinetobacter baumannii strain MRSN548254]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1093360 ACK8DD_RS15975 WP_004738086.1 3384249..3385049(-) (pilF) [Acinetobacter baumannii strain MRSN548254]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment