Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACK8DB_RS16655 Genome accession   NZ_CP179875
Coordinates   3525203..3527431 (+) Length   742 a.a.
NCBI ID   WP_004748437.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN548102     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3520203..3532431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DB_RS16630 (ACK8DB_16630) lnt 3520588..3522147 (+) 1560 WP_004748431.1 apolipoprotein N-acyltransferase -
  ACK8DB_RS16635 (ACK8DB_16635) - 3522149..3522487 (-) 339 WP_000434938.1 tRNA-binding protein -
  ACK8DB_RS16640 (ACK8DB_16640) - 3522632..3522976 (-) 345 WP_000643174.1 hypothetical protein -
  ACK8DB_RS16645 (ACK8DB_16645) gspG 3523244..3523825 (-) 582 WP_000865304.1 type II secretion system major pseudopilin GspG -
  ACK8DB_RS16650 (ACK8DB_16650) gspF 3523849..3525054 (-) 1206 WP_004748434.1 type II secretion system inner membrane protein GspF -
  ACK8DB_RS16655 (ACK8DB_16655) priA 3525203..3527431 (+) 2229 WP_004748437.1 primosomal protein N' Machinery gene
  ACK8DB_RS16660 (ACK8DB_16660) - 3527508..3529349 (+) 1842 WP_000008979.1 monovalent cation:proton antiporter-2 (CPA2) family protein -
  ACK8DB_RS16665 (ACK8DB_16665) - 3529515..3530390 (+) 876 WP_001281765.1 Hsp33 family molecular chaperone HslO -
  ACK8DB_RS16670 (ACK8DB_16670) - 3530528..3531139 (+) 612 WP_004748439.1 LysE family translocator -
  ACK8DB_RS16675 (ACK8DB_16675) - 3531290..3532246 (+) 957 WP_001107916.1 DnaJ C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83930.80 Da        Isoelectric Point: 8.7645

>NTDB_id=1093317 ACK8DB_RS16655 WP_004748437.1 3525203..3527431(+) (priA) [Acinetobacter baumannii strain MRSN548102]
MTITPKPDSVVYRVRVAVPVHLYDTFDYTLTKAQYERAQVGSRVAISFGRQNLIGIITEKVDPQETFTGHFQLKAISELL
DDEPILDEQVLNLLTWSAQYYQFPIGEVMQTALPALLRQGKPMDVLFHLWKIIPCDNAEALLKRSIKQQDAYQILKLHPA
GTTENILNLSGVETATLKALQKKGLVDCTLEPHDFSPSSVQLAQMPLTLNEDQKKATQHVLNAQHQYQAFLLDGLTGSGK
TEVYLHIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFNCDIALLHSGLNDSKRLQAWQQAQTGKASIILGTRSAIYTP
LPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGHLQGCPVLLGSATPSIDSYHLVKTGKLTALQLNQRAGHALLPKMH
LIDLKIVKKQHGISQPLIEQIKNTLARKEQVLIFLNRRGYAPVLVCESCGWQANCPHCDAHFTLHTQPYSYLHCHHCGTV
HRLPDHCPECQQKSLKTLGAGTAKVEEHLQELFPDHDVIRVDRDSTSRVGSWQKIYDRIQQNKPSILLGTQMLAKGHHFP
HVTLVAILDIDAGLLSVDIRAPERTAQLIVQVAGRAGRGEHKGHVYLQTLRPDHPLLTTLIEKDYRAVAKQTLAERKVAL
LPPYRYAVLIRAESKDRDYTLHFLNEAAEQLRQIAGDIVDIWGPIPAPMERKAGRYRAHMVILSADRARLHFYLRQWWAQ
LVHAPRQHQLRLSIDVDPQEFS

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=1093317 ACK8DB_RS16655 WP_004748437.1 3525203..3527431(+) (priA) [Acinetobacter baumannii strain MRSN548102]
ATGACCATAACGCCAAAGCCTGATTCAGTTGTTTATCGTGTGCGTGTTGCCGTACCTGTGCATCTGTACGACACTTTTGA
CTATACACTCACCAAAGCACAATATGAACGGGCTCAAGTAGGTTCACGCGTCGCAATTTCATTTGGACGGCAAAACCTGA
TTGGTATCATTACTGAAAAAGTTGACCCACAAGAAACATTTACAGGTCACTTTCAGCTTAAGGCCATTTCTGAACTTTTA
GATGATGAGCCTATTTTAGATGAACAAGTTTTAAATTTATTGACATGGTCAGCACAATATTACCAATTTCCGATTGGCGA
GGTGATGCAGACTGCTTTACCTGCGTTATTGCGTCAGGGCAAACCCATGGATGTTTTATTTCATCTATGGAAAATCATTC
CATGTGACAATGCCGAAGCTTTATTAAAACGCTCAATCAAACAGCAAGATGCTTATCAAATTTTAAAATTACATCCTGCC
GGAACCACTGAAAATATTCTCAATTTAAGTGGTGTAGAAACTGCAACACTTAAAGCACTACAAAAGAAAGGTCTCGTAGA
CTGTACGCTTGAGCCTCATGACTTTAGTCCATCCTCTGTGCAATTGGCGCAAATGCCACTCACGCTCAATGAAGACCAAA
AGAAGGCCACTCAGCACGTCCTTAATGCACAGCATCAATATCAAGCCTTTTTATTAGATGGTTTAACCGGAAGTGGTAAA
ACTGAAGTTTACCTGCACATCATGCACGAAGTGCTAAAGCAAGGTAAGCAAGTACTGGTGTTAGTTCCCGAAATTGGTTT
AACCCCTCAAACCATTTCGCGCTTTAAGTCTCGTTTTAACTGCGACATTGCTTTATTACATTCGGGGTTAAATGACTCTA
AACGCCTACAAGCCTGGCAACAAGCACAGACTGGAAAGGCCTCTATTATTTTAGGAACACGTTCAGCCATTTATACCCCG
CTGCCACGTTTAGGCCTTATCATTTTAGATGAAGAACACGACCTGTCTTATAAACAACAAGAAGGTTTCCGTTACCATGC
GCGTGATGTCGCGCTTTATCGAGGACATTTACAAGGCTGTCCTGTTCTTTTAGGTTCGGCAACTCCAAGCATTGACAGTT
ATCACTTAGTCAAAACTGGCAAGTTAACTGCACTTCAGCTGAACCAGCGTGCAGGCCATGCGCTTTTACCAAAAATGCAT
TTGATTGATCTTAAAATTGTCAAAAAACAGCATGGTATTAGTCAGCCTTTAATCGAACAGATTAAAAATACCCTAGCAAG
AAAAGAACAGGTTTTAATTTTCTTAAACCGACGTGGTTATGCACCTGTTCTGGTCTGTGAAAGTTGTGGTTGGCAAGCGA
ATTGTCCGCATTGTGATGCGCATTTTACGTTACACACTCAGCCTTATTCTTACTTACATTGTCACCACTGTGGCACAGTT
CATCGCTTGCCAGACCACTGCCCTGAGTGTCAGCAAAAGAGCCTCAAAACTTTAGGTGCTGGCACAGCCAAGGTTGAAGA
GCATTTACAAGAATTATTTCCAGATCATGATGTGATTCGGGTCGATCGTGACAGCACCAGCCGAGTCGGTAGCTGGCAAA
AAATTTATGATCGCATTCAGCAAAATAAACCTTCGATTTTGCTCGGTACGCAAATGTTGGCAAAAGGTCACCATTTTCCG
CATGTGACCTTGGTTGCTATTTTAGACATCGATGCAGGTTTACTCAGTGTCGATATTCGGGCGCCAGAACGTACTGCACA
GTTGATTGTACAAGTGGCTGGTCGTGCAGGTCGTGGAGAACACAAAGGTCATGTTTATTTACAAACATTAAGGCCAGATC
ATCCTTTACTCACCACACTCATCGAAAAAGATTATCGGGCAGTCGCGAAACAAACATTGGCTGAGCGTAAAGTTGCATTA
CTGCCGCCTTATCGCTATGCCGTACTTATACGCGCTGAATCGAAAGATCGCGACTATACTTTGCATTTTTTAAATGAAGC
TGCCGAGCAACTTCGCCAAATTGCAGGTGATATTGTTGATATCTGGGGACCAATTCCAGCGCCAATGGAACGTAAAGCGG
GACGTTACCGCGCACATATGGTGATTTTGTCTGCTGACCGCGCTCGTCTGCATTTTTACTTAAGACAATGGTGGGCTCAG
TTGGTCCATGCGCCAAGACAGCATCAACTCAGACTCTCAATTGATGTTGACCCGCAAGAATTTAGTTAG

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(643-738)

3DNA-binding domain (3BD) (PriA_3primeBD)

(15-114)

Type III restriction enzyme, res subunit (ResIII)

(208-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

97.844

100

0.978

Multiple sequence alignment