Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACK8DA_RS02515 Genome accession   NZ_CP179869
Coordinates   537889..538689 (+) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN122172     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532889..543689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DA_RS02490 (ACK8DA_02490) - 533799..535097 (+) 1299 WP_086264052.1 glycosyltransferase family 4 protein -
  ACK8DA_RS02495 (ACK8DA_02495) - 535122..535673 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  ACK8DA_RS02500 (ACK8DA_02500) iscX 535773..535973 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  ACK8DA_RS02505 (ACK8DA_02505) ndk 536087..536518 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ACK8DA_RS02510 (ACK8DA_02510) rlmN 536641..537873 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACK8DA_RS02515 (ACK8DA_02515) pilF 537889..538689 (+) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACK8DA_RS02520 (ACK8DA_02520) - 538680..539471 (+) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  ACK8DA_RS02525 (ACK8DA_02525) ispG 539493..540608 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACK8DA_RS02530 (ACK8DA_02530) hisS 540605..541897 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  ACK8DA_RS02535 (ACK8DA_02535) - 541926..542624 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=1093199 ACK8DA_RS02515 WP_002027454.1 537889..538689(+) (pilF) [Acinetobacter baumannii strain MRSN122172]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1093199 ACK8DA_RS02515 WP_002027454.1 537889..538689(+) (pilF) [Acinetobacter baumannii strain MRSN122172]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment