Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMGE3_RS12625 Genome accession   NZ_CP179736
Coordinates   2449352..2450713 (-) Length   453 a.a.
NCBI ID   WP_414055507.1    Uniprot ID   -
Organism   Macrococcus sp. 18KM583     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2444352..2455713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGE3_RS12600 (ACMGE3_12600) cysE 2444767..2445408 (-) 642 WP_414045484.1 serine O-acetyltransferase -
  ACMGE3_RS12605 (ACMGE3_12605) gltX 2445667..2447118 (-) 1452 WP_414045483.1 glutamate--tRNA ligase -
  ACMGE3_RS12610 (ACMGE3_12610) ispF 2447132..2447614 (-) 483 WP_414047336.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACMGE3_RS12615 (ACMGE3_12615) ispD 2447611..2448282 (-) 672 WP_414045481.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACMGE3_RS12620 (ACMGE3_12620) - 2448284..2449339 (-) 1056 WP_414045480.1 PIN/TRAM domain-containing protein -
  ACMGE3_RS12625 (ACMGE3_12625) radA 2449352..2450713 (-) 1362 WP_414055507.1 DNA repair protein RadA Machinery gene
  ACMGE3_RS12630 (ACMGE3_12630) - 2450866..2453289 (-) 2424 WP_414055508.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMGE3_RS12635 (ACMGE3_12635) - 2453302..2454312 (-) 1011 WP_414045477.1 protein arginine kinase -
  ACMGE3_RS12640 (ACMGE3_12640) - 2454302..2454694 (-) 393 WP_414055509.1 UvrB/UvrC motif-containing protein -
  ACMGE3_RS12645 (ACMGE3_12645) - 2454706..2455167 (-) 462 WP_414045475.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50262.91 Da        Isoelectric Point: 7.4858

>NTDB_id=1093172 ACMGE3_RS12625 WP_414055507.1 2449352..2450713(-) (radA) [Macrococcus sp. 18KM583]
MAKAKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKTAIHQQDSNSKVEKLKNITKESVPRDHTQMGELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQRHPVLYISGEESVRQTKLRADRLKEDAGELDVYAETNLQIIHETV
KKMQPRFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLTEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVSPTSFHNP
RRMATGIDHNRLTLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIISIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRIIIPKNNIGGWDFPGNIEVIGVTNISEALKLAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1093172 ACMGE3_RS12625 WP_414055507.1 2449352..2450713(-) (radA) [Macrococcus sp. 18KM583]
TTGGCTAAAGCAAAATCAACATTTGAATGTATGGCCTGTGGTTATCAATCCCCAAAATGGATGGGTAAATGTCCAAATTG
TGGTGCCTGGAACCAGATGGAAGAGGTTATTGAACATAAACAAAAAGGTCCGAAGACTGCGATACATCAACAAGATTCAA
ATTCTAAAGTAGAAAAATTAAAAAATATCACTAAGGAAAGTGTTCCGCGGGACCACACACAAATGGGTGAACTTGATCGA
GTATTAGGAGGCGGCATCGTTCCGGGTTCTCTTATATTAATTGGAGGAGACCCTGGTATCGGGAAATCTACATTGCTATT
ACAAGTGTGTGCGATGTTATCTCAGAGACATCCGGTACTATATATATCAGGTGAAGAATCAGTGAGACAGACAAAGCTAA
GAGCTGATAGACTTAAAGAAGATGCTGGCGAACTTGATGTATATGCTGAAACCAATCTGCAGATTATTCACGAGACAGTG
AAGAAAATGCAACCAAGATTTCTGGTGATTGACTCCATTCAGACAATATTCCATCCGGAAGTCACAAGTGCACCAGGATC
TGTATCGCAAGTACGAGAATGTACACAGGAATTAATGCGTATTGCAAAACAGATGAATATTGCCACGTTTATAGTGGGTC
ATGTAACGAAAGAAGGCCAAATTGCTGGTCCGCGCTTACTTGAACATATGGTAGATACCGTATTGTATTTTGAAGGAGAT
ACACATCACAGTTATCGTATACTACGAGCTGTAAAAAACCGTTTTGGTTCGACTAACGAGATGGGTATATTTGAAATGCG
TAACACAGGTTTAACGGAAGTCCTAAATCCGAGCGAGATGTTCCTCGAAGAACGCACGAAAAATGTGGCAGGCTCAACAA
TTGTTGCCACAATGGAAGGCACACGACCATTGCTTGTTGAAGTACAATCACTTGTATCACCAACTTCTTTCCATAATCCA
AGACGTATGGCGACAGGTATAGACCACAACCGTTTAACACTACTTATGGCTGTGCTTGAGAAGAAGCAAGGTTATTTATT
ACAACAGCAGGATGCTTACGTTAAAGTGGCCGGTGGTGTGAAGTTAGATGAACCTGCAGTCGATCTAAGCGTTATTATTA
GTATTGCATCAAGCTATAATGATAAACCGACACGTGGAGATGACTGCTTTATTGGTGAAGTCGGATTAACAGGGGAAGTG
CGTCGGGTAGCACGTATTGAACAACGTGTTCAGGAAGCGGAAAAGCTAGGTTTCAAACGAATCATTATACCGAAGAATAA
CATCGGAGGATGGGATTTCCCAGGTAATATTGAAGTCATCGGTGTAACGAATATTAGTGAAGCATTAAAGCTGGCATTTT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

AAA domain (AAA_25)

(69-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.763

100

0.682
  radA Streptococcus pneumoniae Rx1

62.252

100

0.623
  radA Streptococcus pneumoniae D39

62.252

100

0.623
  radA Streptococcus pneumoniae R6

62.252

100

0.623
  radA Streptococcus pneumoniae TIGR4

62.252

100

0.623
  radA Streptococcus mitis NCTC 12261

62.252

100

0.623
  radA Streptococcus mitis SK321

62.252

100

0.623

Multiple sequence alignment