Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMGE4_RS11850 Genome accession   NZ_CP179735
Coordinates   2224746..2226107 (+) Length   453 a.a.
NCBI ID   WP_414045479.1    Uniprot ID   -
Organism   Macrococcus sp. 18KM445     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2219746..2231107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGE4_RS11830 (ACMGE4_11830) - 2220292..2220753 (+) 462 WP_414045475.1 CtsR family transcriptional regulator -
  ACMGE4_RS11835 (ACMGE4_11835) - 2220765..2221157 (+) 393 WP_414045476.1 UvrB/UvrC motif-containing protein -
  ACMGE4_RS11840 (ACMGE4_11840) - 2221147..2222157 (+) 1011 WP_414045477.1 protein arginine kinase -
  ACMGE4_RS11845 (ACMGE4_11845) - 2222170..2224593 (+) 2424 WP_414045478.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMGE4_RS11850 (ACMGE4_11850) radA 2224746..2226107 (+) 1362 WP_414045479.1 DNA repair protein RadA Machinery gene
  ACMGE4_RS11855 (ACMGE4_11855) - 2226120..2227175 (+) 1056 WP_414045480.1 PIN/TRAM domain-containing protein -
  ACMGE4_RS11860 (ACMGE4_11860) ispD 2227177..2227848 (+) 672 WP_414045481.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACMGE4_RS11865 (ACMGE4_11865) ispF 2227845..2228327 (+) 483 WP_414045482.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACMGE4_RS11870 (ACMGE4_11870) gltX 2228341..2229792 (+) 1452 WP_414045483.1 glutamate--tRNA ligase -
  ACMGE4_RS11875 (ACMGE4_11875) cysE 2230051..2230692 (+) 642 WP_414045484.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50275.95 Da        Isoelectric Point: 7.7237

>NTDB_id=1093138 ACMGE4_RS11850 WP_414045479.1 2224746..2226107(+) (radA) [Macrococcus sp. 18KM445]
MAKAKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKTAIHQQDSNSKVEKLKNITKESVPRDHTQMGELNR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQRHPVLYISGEESVRQTKLRADRLKEDAGELDVYAETNLQIIHETV
KKMQPRFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLTEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVSPTSFHNP
RRMATGIDHNRLTLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSIIISIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRIIIPKNNIGGWDFPGNIEVIGVTNISEALKLAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1093138 ACMGE4_RS11850 WP_414045479.1 2224746..2226107(+) (radA) [Macrococcus sp. 18KM445]
TTGGCTAAAGCAAAATCAACATTTGAATGTATGGCCTGTGGTTATCAATCCCCAAAATGGATGGGTAAATGTCCAAATTG
TGGTGCCTGGAACCAGATGGAAGAGGTTATTGAACATAAACAAAAAGGTCCGAAGACTGCGATACATCAACAAGATTCAA
ATTCTAAAGTAGAAAAATTAAAAAATATCACTAAGGAAAGTGTTCCGCGGGACCACACACAAATGGGTGAACTTAATCGA
GTATTAGGAGGCGGCATCGTTCCGGGTTCTCTTATATTAATTGGAGGAGACCCTGGTATCGGGAAATCTACATTGCTATT
ACAAGTGTGTGCGATGTTATCTCAGAGACATCCGGTACTATATATATCAGGTGAAGAATCAGTGAGACAGACAAAGCTAA
GAGCTGATAGACTTAAAGAAGATGCTGGCGAACTTGATGTATATGCTGAAACCAATCTGCAGATTATTCACGAGACAGTG
AAGAAAATGCAACCAAGATTTCTGGTGATTGACTCCATTCAGACAATATTCCATCCGGAAGTCACAAGTGCACCAGGATC
TGTATCGCAAGTACGAGAATGTACACAGGAATTAATGCGTATTGCAAAACAGATGAATATTGCCACGTTTATAGTGGGTC
ATGTAACGAAAGAAGGCCAAATTGCTGGTCCGCGCTTACTTGAACATATGGTAGATACCGTATTGTATTTTGAAGGAGAT
ACACATCACAGTTATCGTATACTACGAGCTGTAAAAAACCGTTTTGGTTCGACTAACGAGATGGGTATATTTGAAATGCG
TAACACAGGTTTAACGGAAGTCCTAAATCCGAGCGAGATGTTCCTCGAAGAACGCACGAAAAATGTGGCAGGCTCAACAA
TTGTTGCCACAATGGAAGGCACACGACCATTGCTTGTTGAAGTACAATCACTTGTATCACCAACTTCTTTCCATAATCCA
AGACGTATGGCCACAGGTATAGACCACAACCGTTTAACACTACTTATGGCTGTGCTTGAGAAGAAGCAAGGTTATTTATT
ACAACAGCAGGATGCTTACGTTAAAGTGGCCGGTGGTGTGAAGTTAGATGAACCTGCAGTCGATCTAAGCATTATTATTA
GTATTGCATCGAGCTATAATGATAAACCGACACGTGGAGATGATTGCTTTATTGGTGAAGTCGGATTAACAGGGGAAGTG
CGTCGGGTAGCACGTATTGAACAACGTGTTCAGGAAGCGGAAAAGCTAGGTTTCAAACGAATCATTATACCGAAGAATAA
CATCGGAGGATGGGATTTCCCAGGTAATATTGAAGTCATCGGTGTAACGAATATTAGTGAAGCATTAAAGCTGGCATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.202

100

0.687
  radA Streptococcus pneumoniae Rx1

62.252

100

0.623
  radA Streptococcus pneumoniae D39

62.252

100

0.623
  radA Streptococcus pneumoniae R6

62.252

100

0.623
  radA Streptococcus pneumoniae TIGR4

62.252

100

0.623
  radA Streptococcus mitis NCTC 12261

62.252

100

0.623
  radA Streptococcus mitis SK321

62.252

100

0.623

Multiple sequence alignment