Detailed information
Bioinformatically predicted
Overview
| Name | comGA | Type | Machinery gene |
| Locus tag | ACMGE5_RS08225 | Genome accession | NZ_CP179734 |
| Coordinates | 1562718..1563686 (+) | Length | 322 a.a. |
| NCBI ID | WP_414046289.1 | Uniprot ID | - |
| Organism | Macrococcus sp. 18KM444 | ||
| Function | dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 1555916..1616248 | 1562718..1563686 | within | 0 |
Gene organization within MGE regions
Location: 1555916..1616248
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACMGE5_RS08170 (ACMGE5_08170) | pstB | 1555916..1556803 (+) | 888 | WP_414046280.1 | phosphate ABC transporter ATP-binding protein PstB | - |
| ACMGE5_RS08175 (ACMGE5_08175) | phoU | 1556808..1557449 (+) | 642 | WP_414046281.1 | phosphate signaling complex protein PhoU | - |
| ACMGE5_RS08180 (ACMGE5_08180) | rpmG | 1557526..1557675 (+) | 150 | WP_096076353.1 | 50S ribosomal protein L33 | - |
| ACMGE5_RS08185 (ACMGE5_08185) | - | 1557764..1558330 (+) | 567 | WP_414046282.1 | 5-formyltetrahydrofolate cyclo-ligase | - |
| ACMGE5_RS08190 (ACMGE5_08190) | - | 1558327..1559751 (+) | 1425 | WP_414046283.1 | rhomboid family intramembrane serine protease | - |
| ACMGE5_RS08195 (ACMGE5_08195) | - | 1559756..1559947 (+) | 192 | WP_414046284.1 | YqgQ family protein | - |
| ACMGE5_RS08200 (ACMGE5_08200) | - | 1559944..1560915 (+) | 972 | WP_414046285.1 | ROK family glucokinase | - |
| ACMGE5_RS08205 (ACMGE5_08205) | - | 1560915..1561226 (+) | 312 | WP_414046286.1 | MTH1187 family thiamine-binding protein | - |
| ACMGE5_RS08210 (ACMGE5_08210) | - | 1561260..1561499 (-) | 240 | WP_414046287.1 | DUF2759 domain-containing protein | - |
| ACMGE5_RS08215 (ACMGE5_08215) | - | 1561592..1562221 (+) | 630 | WP_414046288.1 | MBL fold metallo-hydrolase | - |
| ACMGE5_RS08220 (ACMGE5_08220) | - | 1562257..1562505 (-) | 249 | WP_233680344.1 | DUF2626 domain-containing protein | - |
| ACMGE5_RS08225 (ACMGE5_08225) | comGA | 1562718..1563686 (+) | 969 | WP_414046289.1 | competence type IV pilus ATPase ComGA | Machinery gene |
| ACMGE5_RS08230 (ACMGE5_08230) | comGB | 1563673..1564710 (+) | 1038 | WP_414046290.1 | competence type IV pilus assembly protein ComGB | - |
| ACMGE5_RS08235 (ACMGE5_08235) | comGC | 1564722..1565072 (+) | 351 | WP_414046291.1 | competence type IV pilus major pilin ComGC | Machinery gene |
| ACMGE5_RS08240 (ACMGE5_08240) | - | 1565038..1565490 (+) | 453 | WP_414046852.1 | hypothetical protein | - |
| ACMGE5_RS08245 (ACMGE5_08245) | - | 1565501..1565722 (+) | 222 | WP_414046293.1 | hypothetical protein | - |
| ACMGE5_RS08250 (ACMGE5_08250) | comGF | 1565685..1566116 (+) | 432 | WP_414046294.1 | competence type IV pilus minor pilin ComGF | - |
| ACMGE5_RS08255 (ACMGE5_08255) | - | 1566297..1566803 (+) | 507 | WP_414046295.1 | shikimate kinase | - |
| ACMGE5_RS08260 (ACMGE5_08260) | gcvT | 1566937..1568022 (+) | 1086 | WP_414046784.1 | glycine cleavage system aminomethyltransferase GcvT | - |
| ACMGE5_RS08265 (ACMGE5_08265) | gcvPA | 1568035..1569378 (+) | 1344 | WP_414046296.1 | aminomethyl-transferring glycine dehydrogenase subunit GcvPA | - |
| ACMGE5_RS08270 (ACMGE5_08270) | gcvPB | 1569371..1570840 (+) | 1470 | WP_414046297.1 | aminomethyl-transferring glycine dehydrogenase subunit GcvPB | - |
| ACMGE5_RS08275 (ACMGE5_08275) | - | 1570885..1571268 (-) | 384 | WP_414046298.1 | rhodanese-like domain-containing protein | - |
| ACMGE5_RS08280 (ACMGE5_08280) | - | 1571376..1572218 (+) | 843 | WP_414046299.1 | biotin/lipoate A/B protein ligase family protein | - |
| ACMGE5_RS08285 (ACMGE5_08285) | - | 1572273..1572863 (-) | 591 | WP_414046300.1 | DUF6376 family protein | - |
| ACMGE5_RS08290 (ACMGE5_08290) | - | 1572955..1574010 (+) | 1056 | WP_414046301.1 | M24 family metallopeptidase | - |
| ACMGE5_RS08295 (ACMGE5_08295) | efp | 1574036..1574593 (+) | 558 | WP_096076375.1 | elongation factor P | - |
| ACMGE5_RS08300 (ACMGE5_08300) | accB | 1574709..1575164 (+) | 456 | WP_414046302.1 | acetyl-CoA carboxylase biotin carboxyl carrier protein | - |
| ACMGE5_RS08305 (ACMGE5_08305) | accC | 1575167..1576513 (+) | 1347 | WP_414046303.1 | acetyl-CoA carboxylase biotin carboxylase subunit | - |
| ACMGE5_RS08310 (ACMGE5_08310) | - | 1576602..1576949 (+) | 348 | WP_414046304.1 | Asp23/Gls24 family envelope stress response protein | - |
| ACMGE5_RS08315 (ACMGE5_08315) | nusB | 1576994..1577386 (+) | 393 | WP_414046305.1 | transcription antitermination factor NusB | - |
| ACMGE5_RS08320 (ACMGE5_08320) | xseA | 1577394..1578731 (+) | 1338 | WP_414046306.1 | exodeoxyribonuclease VII large subunit | - |
| ACMGE5_RS08325 (ACMGE5_08325) | xseB | 1578734..1578928 (+) | 195 | WP_414046307.1 | exodeoxyribonuclease VII small subunit | - |
| ACMGE5_RS08330 (ACMGE5_08330) | - | 1578921..1579781 (+) | 861 | WP_414046308.1 | polyprenyl synthetase family protein | - |
| ACMGE5_RS08335 (ACMGE5_08335) | dxs | 1579877..1581763 (+) | 1887 | WP_414046309.1 | 1-deoxy-D-xylulose-5-phosphate synthase | - |
| ACMGE5_RS08340 (ACMGE5_08340) | - | 1581750..1582580 (+) | 831 | WP_414046310.1 | TlyA family RNA methyltransferase | - |
| ACMGE5_RS08345 (ACMGE5_08345) | ahrC | 1582676..1583128 (+) | 453 | WP_414046311.1 | transcriptional regulator AhrC/ArgR | - |
| ACMGE5_RS08350 (ACMGE5_08350) | recN | 1583142..1584809 (+) | 1668 | WP_414046312.1 | DNA repair protein RecN | - |
| ACMGE5_RS08355 (ACMGE5_08355) | - | 1584827..1585519 (+) | 693 | WP_414046313.1 | response regulator | - |
| ACMGE5_RS08360 (ACMGE5_08360) | - | 1585512..1586243 (+) | 732 | WP_414046314.1 | glycerophosphodiester phosphodiesterase family protein | - |
| ACMGE5_RS08365 (ACMGE5_08365) | - | 1586286..1586522 (-) | 237 | WP_414046315.1 | DUF2627 domain-containing protein | - |
| ACMGE5_RS08370 (ACMGE5_08370) | - | 1586840..1587940 (+) | 1101 | WP_414046316.1 | Glu/Leu/Phe/Val dehydrogenase dimerization domain-containing protein | - |
| ACMGE5_RS08375 (ACMGE5_08375) | - | 1588001..1588204 (+) | 204 | WP_414046317.1 | hypothetical protein | - |
| ACMGE5_RS08380 (ACMGE5_08380) | - | 1588290..1588793 (+) | 504 | WP_414046318.1 | DJ-1/PfpI family protein | - |
| ACMGE5_RS08385 (ACMGE5_08385) | - | 1588880..1589401 (+) | 522 | WP_414046319.1 | tunicamycin resistance protein | - |
| ACMGE5_RS08390 (ACMGE5_08390) | - | 1589950..1590381 (-) | 432 | WP_414046320.1 | LytTR family transcriptional regulator DNA-binding domain-containing protein | - |
| ACMGE5_RS08395 (ACMGE5_08395) | - | 1590558..1591064 (+) | 507 | WP_414046321.1 | GrpB family protein | - |
| ACMGE5_RS08400 (ACMGE5_08400) | - | 1591093..1591278 (-) | 186 | WP_233680313.1 | helix-turn-helix transcriptional regulator | - |
| ACMGE5_RS08405 (ACMGE5_08405) | - | 1591285..1591614 (-) | 330 | WP_414046322.1 | hypothetical protein | - |
| ACMGE5_RS08410 (ACMGE5_08410) | - | 1591628..1591939 (-) | 312 | WP_414046323.1 | hypothetical protein | - |
| ACMGE5_RS08415 (ACMGE5_08415) | - | 1592117..1592866 (+) | 750 | WP_414046324.1 | nucleoside phosphorylase | - |
| ACMGE5_RS08420 (ACMGE5_08420) | - | 1592953..1593912 (-) | 960 | WP_414046325.1 | IS30 family transposase | - |
| ACMGE5_RS08425 (ACMGE5_08425) | - | 1593983..1594369 (+) | 387 | WP_414046326.1 | hypothetical protein | - |
| ACMGE5_RS08430 (ACMGE5_08430) | - | 1594596..1595225 (+) | 630 | WP_414046327.1 | FusB/FusC family EF-G-binding protein | - |
| ACMGE5_RS08435 (ACMGE5_08435) | - | 1595243..1595488 (-) | 246 | WP_414046853.1 | hypothetical protein | - |
| ACMGE5_RS08440 (ACMGE5_08440) | - | 1595508..1596794 (-) | 1287 | WP_414046328.1 | RtcB family protein | - |
| ACMGE5_RS08445 (ACMGE5_08445) | - | 1597236..1597907 (+) | 672 | WP_414046329.1 | type 1 glutamine amidotransferase domain-containing protein | - |
| ACMGE5_RS08450 (ACMGE5_08450) | - | 1598061..1598975 (+) | 915 | WP_414046330.1 | hypothetical protein | - |
| ACMGE5_RS08455 (ACMGE5_08455) | - | 1598989..1599201 (+) | 213 | WP_414046331.1 | hypothetical protein | - |
| ACMGE5_RS08460 (ACMGE5_08460) | - | 1599269..1599751 (-) | 483 | WP_414046332.1 | CHAP domain-containing protein | - |
| ACMGE5_RS08465 (ACMGE5_08465) | - | 1599748..1600230 (-) | 483 | WP_414046333.1 | GNAT family N-acetyltransferase | - |
| ACMGE5_RS08470 (ACMGE5_08470) | - | 1600527..1600964 (+) | 438 | WP_414046334.1 | BrxA/BrxB family bacilliredoxin | - |
| ACMGE5_RS08475 (ACMGE5_08475) | - | 1600972..1601940 (+) | 969 | WP_414046335.1 | aromatic acid exporter family protein | - |
| ACMGE5_RS08480 (ACMGE5_08480) | - | 1602189..1603292 (+) | 1104 | WP_414046336.1 | M20/M25/M40 family metallo-hydrolase | - |
| ACMGE5_RS08485 (ACMGE5_08485) | gndA | 1603353..1604756 (+) | 1404 | WP_414046337.1 | NADP-dependent phosphogluconate dehydrogenase | - |
| ACMGE5_RS08490 (ACMGE5_08490) | - | 1604881..1606233 (+) | 1353 | WP_414046854.1 | IS1182 family transposase | - |
| ACMGE5_RS08495 (ACMGE5_08495) | - | 1606360..1607196 (-) | 837 | WP_414046338.1 | helix-turn-helix domain-containing protein | - |
| ACMGE5_RS08500 (ACMGE5_08500) | - | 1607224..1607853 (-) | 630 | WP_414046339.1 | hypothetical protein | - |
| ACMGE5_RS08505 (ACMGE5_08505) | zwf | 1607926..1609404 (-) | 1479 | WP_414046340.1 | glucose-6-phosphate dehydrogenase | - |
| ACMGE5_RS08510 (ACMGE5_08510) | rnz | 1609513..1610433 (+) | 921 | WP_414046341.1 | ribonuclease Z | - |
| ACMGE5_RS08515 (ACMGE5_08515) | proC | 1610505..1611308 (-) | 804 | WP_414046342.1 | pyrroline-5-carboxylate reductase | - |
| ACMGE5_RS08520 (ACMGE5_08520) | - | 1611390..1612166 (+) | 777 | WP_414046343.1 | SDR family NAD(P)-dependent oxidoreductase | - |
| ACMGE5_RS08525 (ACMGE5_08525) | - | 1612182..1613171 (-) | 990 | WP_414046344.1 | alpha/beta hydrolase | - |
| ACMGE5_RS08530 (ACMGE5_08530) | - | 1613168..1614085 (-) | 918 | WP_414046345.1 | aldo/keto reductase | - |
| ACMGE5_RS08535 (ACMGE5_08535) | - | 1614149..1614694 (+) | 546 | WP_414046346.1 | NUDIX hydrolase | - |
| ACMGE5_RS08540 (ACMGE5_08540) | - | 1614785..1615264 (+) | 480 | WP_414046347.1 | Fur family transcriptional regulator | - |
| ACMGE5_RS08545 (ACMGE5_08545) | xerD | 1615361..1616248 (+) | 888 | WP_414046348.1 | site-specific tyrosine recombinase XerD | - |
Sequence
Protein
Download Length: 322 a.a. Molecular weight: 36322.67 Da Isoelectric Point: 6.6856
>NTDB_id=1093097 ACMGE5_RS08225 WP_414046289.1 1562718..1563686(+) (comGA) [Macrococcus sp. 18KM444]
MEKLFTSILKDAILQQASDVHFIPSQKSVLIKLRIHGDMHDYDKIAINQFEKLLSYLKFITNLDVSEKNKAQSGTLNFDL
NAIHYNVRASTLPHSVGHEACVLRIIPTNFNEKADIEDDLLHLMHKGSGLILISGPTGSGKSTLMYHLLQYAVANLSKQV
ISIEDPVEQRIEGMIQINVNAKGNITYQNSLKAILRCDPDIIMIGEIRDADTAKNVINASLSGHLVLSTLHANSCLGAIH
RLMEMGISKVELEQSVERIINQRLVNTCSGRRRVYEQLNKSECRNYLSDQSLDSYLPINNQLNKMLDNNEITLMEHERYV
TE
MEKLFTSILKDAILQQASDVHFIPSQKSVLIKLRIHGDMHDYDKIAINQFEKLLSYLKFITNLDVSEKNKAQSGTLNFDL
NAIHYNVRASTLPHSVGHEACVLRIIPTNFNEKADIEDDLLHLMHKGSGLILISGPTGSGKSTLMYHLLQYAVANLSKQV
ISIEDPVEQRIEGMIQINVNAKGNITYQNSLKAILRCDPDIIMIGEIRDADTAKNVINASLSGHLVLSTLHANSCLGAIH
RLMEMGISKVELEQSVERIINQRLVNTCSGRRRVYEQLNKSECRNYLSDQSLDSYLPINNQLNKMLDNNEITLMEHERYV
TE
Nucleotide
Download Length: 969 bp
>NTDB_id=1093097 ACMGE5_RS08225 WP_414046289.1 1562718..1563686(+) (comGA) [Macrococcus sp. 18KM444]
ATGGAAAAACTATTTACTTCAATATTAAAAGATGCAATATTACAACAAGCCTCAGATGTTCACTTTATACCCAGTCAAAA
GAGTGTATTAATCAAATTACGAATCCATGGTGATATGCATGATTATGACAAAATAGCTATCAATCAATTTGAAAAATTAT
TGTCTTATCTAAAGTTTATCACCAACCTTGATGTTTCTGAAAAAAATAAAGCACAAAGCGGAACCCTGAATTTTGATTTA
AATGCTATTCATTATAATGTGCGTGCTTCTACATTACCTCATTCGGTAGGTCATGAAGCATGTGTTTTAAGGATAATTCC
TACCAATTTTAATGAAAAAGCAGATATTGAAGATGATTTATTACATTTAATGCATAAAGGTAGTGGTCTGATTTTAATAA
GTGGACCAACGGGTAGTGGTAAGAGTACATTAATGTATCATCTGCTTCAATATGCAGTTGCAAATTTAAGTAAACAAGTG
ATATCGATAGAGGATCCTGTTGAACAAAGGATTGAAGGTATGATACAAATTAACGTCAACGCAAAAGGGAATATCACCTA
TCAGAATTCACTCAAAGCAATATTAAGATGTGATCCGGATATTATTATGATTGGCGAAATACGGGACGCAGATACCGCTA
AAAATGTAATCAATGCGAGTTTAAGTGGCCATCTAGTATTATCGACACTACATGCAAATTCCTGTTTAGGGGCAATACAT
CGATTGATGGAAATGGGCATCTCAAAAGTTGAACTTGAACAGAGTGTTGAAAGAATAATAAATCAAAGATTAGTAAACAC
TTGTTCTGGTAGAAGACGTGTTTACGAACAACTAAATAAGAGTGAATGTCGCAATTATTTATCTGATCAGTCATTAGATT
CATATCTCCCTATTAATAATCAATTAAATAAGATGCTTGATAATAATGAGATAACTTTAATGGAGCATGAACGTTATGTT
ACTGAGTAG
ATGGAAAAACTATTTACTTCAATATTAAAAGATGCAATATTACAACAAGCCTCAGATGTTCACTTTATACCCAGTCAAAA
GAGTGTATTAATCAAATTACGAATCCATGGTGATATGCATGATTATGACAAAATAGCTATCAATCAATTTGAAAAATTAT
TGTCTTATCTAAAGTTTATCACCAACCTTGATGTTTCTGAAAAAAATAAAGCACAAAGCGGAACCCTGAATTTTGATTTA
AATGCTATTCATTATAATGTGCGTGCTTCTACATTACCTCATTCGGTAGGTCATGAAGCATGTGTTTTAAGGATAATTCC
TACCAATTTTAATGAAAAAGCAGATATTGAAGATGATTTATTACATTTAATGCATAAAGGTAGTGGTCTGATTTTAATAA
GTGGACCAACGGGTAGTGGTAAGAGTACATTAATGTATCATCTGCTTCAATATGCAGTTGCAAATTTAAGTAAACAAGTG
ATATCGATAGAGGATCCTGTTGAACAAAGGATTGAAGGTATGATACAAATTAACGTCAACGCAAAAGGGAATATCACCTA
TCAGAATTCACTCAAAGCAATATTAAGATGTGATCCGGATATTATTATGATTGGCGAAATACGGGACGCAGATACCGCTA
AAAATGTAATCAATGCGAGTTTAAGTGGCCATCTAGTATTATCGACACTACATGCAAATTCCTGTTTAGGGGCAATACAT
CGATTGATGGAAATGGGCATCTCAAAAGTTGAACTTGAACAGAGTGTTGAAAGAATAATAAATCAAAGATTAGTAAACAC
TTGTTCTGGTAGAAGACGTGTTTACGAACAACTAAATAAGAGTGAATGTCGCAATTATTTATCTGATCAGTCATTAGATT
CATATCTCCCTATTAATAATCAATTAAATAAGATGCTTGATAATAATGAGATAACTTTAATGGAGCATGAACGTTATGTT
ACTGAGTAG
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| comGA | Staphylococcus aureus MW2 |
50.769 |
100 |
0.512 |
| comGA | Staphylococcus aureus N315 |
50.769 |
100 |
0.512 |