Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMGE7_RS11250 Genome accession   NZ_CP179731
Coordinates   2190628..2191989 (-) Length   453 a.a.
NCBI ID   WP_414047337.1    Uniprot ID   -
Organism   Macrococcus sp. 18KM245     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2185628..2196989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGE7_RS11225 (ACMGE7_11225) cysE 2186043..2186684 (-) 642 WP_414045484.1 serine O-acetyltransferase -
  ACMGE7_RS11230 (ACMGE7_11230) gltX 2186943..2188394 (-) 1452 WP_414045483.1 glutamate--tRNA ligase -
  ACMGE7_RS11235 (ACMGE7_11235) ispF 2188408..2188890 (-) 483 WP_414047336.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACMGE7_RS11240 (ACMGE7_11240) ispD 2188887..2189558 (-) 672 WP_414045481.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACMGE7_RS11245 (ACMGE7_11245) - 2189560..2190615 (-) 1056 WP_414045480.1 PIN/TRAM domain-containing protein -
  ACMGE7_RS11250 (ACMGE7_11250) radA 2190628..2191989 (-) 1362 WP_414047337.1 DNA repair protein RadA Machinery gene
  ACMGE7_RS11255 (ACMGE7_11255) - 2192142..2194565 (-) 2424 WP_414047338.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMGE7_RS11260 (ACMGE7_11260) - 2194578..2195588 (-) 1011 WP_414047339.1 protein arginine kinase -
  ACMGE7_RS11265 (ACMGE7_11265) - 2195578..2195970 (-) 393 WP_414045476.1 UvrB/UvrC motif-containing protein -
  ACMGE7_RS11270 (ACMGE7_11270) - 2195982..2196443 (-) 462 WP_414047340.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50276.93 Da        Isoelectric Point: 7.4858

>NTDB_id=1093033 ACMGE7_RS11250 WP_414047337.1 2190628..2191989(-) (radA) [Macrococcus sp. 18KM245]
MAKAKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKTAIHQQDSNSKVEKLKNITKESVPRDHTQMGELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQRHPVLYISGEESVRQTKLRADRLKEDAGELDVYAETNLQIIHETV
KKMQPRFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLTEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVSPTSFHNP
RRMATGIDHNRLTLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSIIISIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRIIIPKNNIGGWDFPGNIEVIGVTNISEALKLAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1093033 ACMGE7_RS11250 WP_414047337.1 2190628..2191989(-) (radA) [Macrococcus sp. 18KM245]
TTGGCTAAAGCAAAATCAACATTTGAATGTATGGCCTGTGGTTATCAATCCCCAAAATGGATGGGTAAATGTCCAAATTG
TGGTGCCTGGAACCAGATGGAAGAGGTTATTGAACATAAACAAAAAGGTCCGAAGACTGCGATACATCAACAAGATTCAA
ATTCTAAAGTAGAAAAATTAAAAAATATCACTAAGGAAAGTGTTCCGCGGGACCACACACAAATGGGTGAACTTGATCGA
GTATTAGGAGGCGGCATCGTTCCGGGTTCTCTTATATTAATTGGAGGAGACCCTGGTATCGGGAAATCTACATTGCTATT
ACAAGTGTGTGCGATGTTATCTCAGAGACATCCGGTACTATATATATCAGGTGAAGAATCAGTGAGACAGACAAAACTAA
GAGCTGATAGACTTAAAGAAGATGCTGGCGAACTTGATGTATATGCTGAAACCAATCTGCAGATTATTCACGAGACAGTG
AAGAAAATGCAACCAAGATTTCTGGTGATTGACTCCATTCAGACAATATTCCATCCGGAAGTCACAAGTGCACCAGGATC
TGTATCGCAAGTACGAGAATGTACACAGGAATTAATGCGTATTGCAAAACAGATGAATATTGCCACGTTTATAGTGGGTC
ATGTAACGAAAGAAGGCCAAATTGCTGGTCCGCGCTTACTTGAACATATGGTAGATACCGTATTGTATTTTGAAGGAGAT
ACACATCACAGTTATCGTATACTACGAGCTGTAAAAAACCGTTTTGGTTCGACTAACGAGATGGGTATATTTGAAATGCG
TAACACAGGTTTAACGGAAGTCCTAAATCCGAGCGAGATGTTCCTCGAAGAACGCACGAAAAATGTGGCAGGCTCAACAA
TTGTTGCCACAATGGAAGGCACACGACCATTGCTTGTTGAAGTACAATCACTTGTATCACCAACTTCTTTCCATAATCCA
AGACGTATGGCCACAGGTATAGACCACAACCGTTTAACACTACTTATGGCTGTGCTTGAGAAGAAGCAAGGTTATTTATT
ACAACAGCAGGATGCTTACGTTAAAGTGGCCGGTGGTGTGAAGTTAGATGAACCTGCAGTCGATCTAAGCATTATTATTA
GTATTGCATCGAGCTATAATGATAAACCGACACGTGGAGATGATTGCTTTATTGGTGAAGTCGGATTAACAGGGGAAGTG
CGTCGGGTAGCACGTATTGAACAACGTGTTCAGGAAGCGGAAAAGCTAGGTTTCAAACGAATCATTATACCGAAGAATAA
CATCGGAGGATGGGATTTCCCAGGTAATATTGAAGTCATCGGTGTAACGAATATTAGTGAAGCATTAAAGCTGGCATTTT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.982

100

0.684
  radA Streptococcus pneumoniae Rx1

62.031

100

0.62
  radA Streptococcus pneumoniae D39

62.031

100

0.62
  radA Streptococcus pneumoniae R6

62.031

100

0.62
  radA Streptococcus pneumoniae TIGR4

62.031

100

0.62
  radA Streptococcus mitis NCTC 12261

62.031

100

0.62
  radA Streptococcus mitis SK321

62.031

100

0.62

Multiple sequence alignment