Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMGFA_RS11675 Genome accession   NZ_CP179725
Coordinates   2171747..2173108 (-) Length   453 a.a.
NCBI ID   WP_414042127.1    Uniprot ID   -
Organism   Macrococcus sp. JEK85     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2166747..2178108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGFA_RS11650 (ACMGFA_11650) cysE 2167165..2167806 (-) 642 WP_414042132.1 serine O-acetyltransferase -
  ACMGFA_RS11655 (ACMGFA_11655) gltX 2168062..2169513 (-) 1452 WP_414042131.1 glutamate--tRNA ligase -
  ACMGFA_RS11660 (ACMGFA_11660) ispF 2169527..2170009 (-) 483 WP_414050663.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACMGFA_RS11665 (ACMGFA_11665) ispD 2170006..2170686 (-) 681 WP_414042129.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACMGFA_RS11670 (ACMGFA_11670) - 2170679..2171734 (-) 1056 WP_414042128.1 PIN/TRAM domain-containing protein -
  ACMGFA_RS11675 (ACMGFA_11675) radA 2171747..2173108 (-) 1362 WP_414042127.1 DNA repair protein RadA Machinery gene
  ACMGFA_RS11680 (ACMGFA_11680) - 2173260..2175683 (-) 2424 WP_414042126.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMGFA_RS11685 (ACMGFA_11685) - 2175696..2176712 (-) 1017 WP_414044466.1 protein arginine kinase -
  ACMGFA_RS11690 (ACMGFA_11690) - 2176696..2177088 (-) 393 WP_414042125.1 UvrB/UvrC motif-containing protein -
  ACMGFA_RS11695 (ACMGFA_11695) - 2177101..2177562 (-) 462 WP_414042124.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50188.82 Da        Isoelectric Point: 7.5063

>NTDB_id=1092937 ACMGFA_RS11675 WP_414042127.1 2171747..2173108(-) (radA) [Macrococcus sp. JEK85]
MAKAKSNFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKTAIHQQDSNSKVEKLKNITKESVPRVHTQMSELDR
VLGGGIVPGSLVLIGGDPGIGKSTLLLQVCAMLSQHHPVLYISGEESVRQTKLRADRLKEDAGELDVYAETNLQIIHETV
KKMQPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLTEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVSPTSFHNP
RRMATGIDHNRLTLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSIIISIASSYNDKSTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNIGEALKLAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1092937 ACMGFA_RS11675 WP_414042127.1 2171747..2173108(-) (radA) [Macrococcus sp. JEK85]
TTGGCTAAAGCAAAATCGAACTTTGAATGTATGGCCTGTGGTTACCAGTCCCCAAAATGGATGGGAAAGTGTCCTAATTG
TGGTGCTTGGAATCAAATGGAAGAGGTCATTGAGCACAAACAAAAAGGTCCAAAAACAGCAATACATCAACAGGATTCAA
ATTCAAAAGTAGAAAAATTAAAAAATATTACGAAAGAAAGTGTGCCACGTGTCCATACGCAAATGAGTGAACTTGACCGT
GTGCTAGGTGGAGGCATTGTTCCGGGATCACTTGTTCTGATTGGTGGGGATCCAGGTATAGGTAAGTCTACACTGTTACT
TCAAGTATGTGCAATGTTATCACAGCATCATCCTGTATTGTACATTTCAGGTGAAGAGTCAGTACGTCAAACGAAATTAC
GTGCAGACCGTTTGAAAGAAGATGCGGGCGAATTAGATGTTTACGCAGAAACTAATCTACAAATCATTCATGAAACAGTT
AAGAAGATGCAGCCGAAGTTCTTAGTAATCGACTCTATTCAAACGATTTTTCATCCGGAAGTCACAAGTGCTCCAGGCTC
AGTTTCTCAAGTGAGAGAATGTACGCAAGAATTAATGCGTATTGCAAAACAGATGAATATCGCAACATTCATTGTGGGTC
ACGTAACAAAAGAAGGCCAAATTGCAGGACCACGTTTGCTTGAACATATGGTGGATACAGTACTGTATTTTGAAGGAGAT
ACACATCATAGCTATCGTATATTACGTGCAGTGAAAAACCGTTTCGGATCTACTAATGAAATGGGTATATTTGAGATGCG
CAACACTGGTTTAACAGAAGTATTGAATCCAAGTGAAATGTTCTTAGAAGAGCGTACGAAGAATGTAGCGGGTTCTACGA
TTGTTGCGACAATGGAAGGGACTCGTCCACTTCTCGTTGAAGTGCAGTCACTAGTGTCACCAACGTCTTTCCATAATCCG
AGACGAATGGCGACAGGAATTGACCATAATCGTTTAACTCTTCTTATGGCTGTCCTTGAGAAGAAACAAGGGTATTTGCT
GCAACAACAAGATGCTTATGTTAAAGTTGCAGGTGGTGTGAAGCTAGACGAACCAGCTGTTGATTTAAGCATTATTATTA
GTATTGCTTCTAGTTATAATGATAAATCGACGCGTGGCGACGACTGTTTCATCGGTGAGGTTGGATTAACTGGTGAAGTA
AGGCGTGTCGCACGTATTGAACAGCGTGTACAAGAAGCAGAAAAACTAGGCTTTAAACGCGTTATTATTCCAAAGAATAA
TATCGGTGGATGGGATTTCCCAGGCAATATCGAAGTAATCGGAGTAACGAATATCGGTGAGGCATTAAAACTTGCATTTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.982

100

0.684
  radA Streptococcus pneumoniae Rx1

61.589

100

0.616
  radA Streptococcus pneumoniae D39

61.589

100

0.616
  radA Streptococcus pneumoniae R6

61.589

100

0.616
  radA Streptococcus pneumoniae TIGR4

61.589

100

0.616
  radA Streptococcus mitis SK321

61.589

100

0.616
  radA Streptococcus mitis NCTC 12261

61.589

100

0.616

Multiple sequence alignment