Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACMGFB_RS09755 Genome accession   NZ_CP179724
Coordinates   1809767..1811128 (-) Length   453 a.a.
NCBI ID   WP_414048853.1    Uniprot ID   -
Organism   Macrococcus sp. 21M1142     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1804767..1816128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGFB_RS09730 (ACMGFB_09730) cysE 1805185..1805826 (-) 642 WP_414042132.1 serine O-acetyltransferase -
  ACMGFB_RS09735 (ACMGFB_09735) gltX 1806082..1807533 (-) 1452 WP_414042131.1 glutamate--tRNA ligase -
  ACMGFB_RS09740 (ACMGFB_09740) ispF 1807547..1808029 (-) 483 WP_414048850.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACMGFB_RS09745 (ACMGFB_09745) ispD 1808026..1808706 (-) 681 WP_414048851.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACMGFB_RS09750 (ACMGFB_09750) - 1808699..1809754 (-) 1056 WP_414048852.1 PIN/TRAM domain-containing protein -
  ACMGFB_RS09755 (ACMGFB_09755) radA 1809767..1811128 (-) 1362 WP_414048853.1 DNA repair protein RadA Machinery gene
  ACMGFB_RS09760 (ACMGFB_09760) - 1811280..1813703 (-) 2424 WP_414048854.1 ATP-dependent Clp protease ATP-binding subunit -
  ACMGFB_RS09765 (ACMGFB_09765) - 1813716..1814732 (-) 1017 WP_414044466.1 protein arginine kinase -
  ACMGFB_RS09770 (ACMGFB_09770) - 1814716..1815108 (-) 393 WP_414042125.1 UvrB/UvrC motif-containing protein -
  ACMGFB_RS09775 (ACMGFB_09775) - 1815121..1815582 (-) 462 WP_414048855.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50168.84 Da        Isoelectric Point: 7.5063

>NTDB_id=1092903 ACMGFB_RS09755 WP_414048853.1 1809767..1811128(-) (radA) [Macrococcus sp. 21M1142]
MAKAKSNFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKTAIHQQDSNSKVEKLKNITKESVPRVHTQMGELDR
VLGGGIVPGSLVLIGGDPGIGKSTLLLQVCAMLSQHHPVLYISGEESVRQTKLRADRLKEDAGELDVYAETNLQIIHETV
KKMQPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLTEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVSPTSFHNP
RRMATGIDHNRLTLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSIIISIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNIGEALKLAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1092903 ACMGFB_RS09755 WP_414048853.1 1809767..1811128(-) (radA) [Macrococcus sp. 21M1142]
TTGGCTAAAGCAAAATCGAACTTTGAATGTATGGCCTGTGGTTACCAGTCCCCAAAATGGATGGGAAAGTGTCCTAATTG
TGGTGCTTGGAATCAAATGGAAGAGGTCATTGAGCACAAACAAAAAGGTCCAAAAACAGCAATACATCAACAGGATTCAA
ATTCAAAAGTAGAAAAATTAAAAAATATTACGAAAGAAAGTGTGCCCCGTGTCCATACGCAAATGGGTGAACTTGACCGT
GTGCTAGGCGGAGGCATTGTTCCGGGATCACTTGTTCTGATTGGTGGGGATCCAGGTATAGGTAAGTCTACACTACTACT
TCAAGTATGTGCAATGTTATCACAGCATCATCCTGTATTGTACATTTCAGGGGAAGAGTCAGTACGTCAAACGAAATTAC
GTGCAGACCGTTTGAAAGAAGATGCGGGCGAATTAGATGTTTACGCAGAAACTAATCTACAAATCATTCATGAAACAGTT
AAGAAGATGCAGCCGAAGTTCTTAGTAATCGACTCTATTCAAACGATTTTTCATCCGGAAGTCACAAGTGCTCCAGGCTC
AGTTTCTCAAGTGAGAGAATGTACGCAAGAATTAATGCGTATTGCAAAACAGATGAATATCGCAACATTCATTGTGGGTC
ACGTAACAAAAGAAGGCCAAATTGCAGGACCACGTTTGCTTGAACATATGGTGGATACAGTACTGTATTTTGAAGGAGAT
ACACATCATAGCTATCGTATATTACGTGCAGTGAAAAACCGTTTCGGATCTACTAATGAAATGGGTATATTTGAGATGCG
CAACACTGGTTTAACAGAAGTATTGAATCCAAGTGAAATGTTCTTAGAAGAGCGTACGAAGAATGTAGCGGGTTCTACGA
TTGTTGCGACAATGGAAGGGACTCGTCCACTTCTCGTTGAAGTGCAGTCACTAGTGTCACCAACGTCTTTCCATAATCCG
AGACGAATGGCGACAGGAATTGATCATAACCGATTAACACTCCTTATGGCTGTGCTCGAGAAGAAACAAGGGTATTTACT
GCAACAACAAGATGCTTATGTTAAAGTTGCAGGTGGTGTGAAGCTAGATGAACCAGCTGTTGATTTAAGTATTATTATTA
GTATTGCTTCTAGCTATAACGATAAACCGACGCGTGGTGACGACTGTTTCATCGGTGAGGTTGGATTAACTGGTGAAGTA
AGGCGTGTCGCACGTATTGAACAGCGTGTACAAGAAGCAGAAAAACTAGGCTTTAAACGCGTTATTATACCGAAGAATAA
TATCGGTGGATGGGATTTCCCAGGCAATATCGAAGTAATCGGAGTAACGAATATCGGCGAGGCGTTAAAACTTGCATTTT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.421

100

0.689
  radA Streptococcus pneumoniae Rx1

61.81

100

0.618
  radA Streptococcus pneumoniae D39

61.81

100

0.618
  radA Streptococcus pneumoniae R6

61.81

100

0.618
  radA Streptococcus pneumoniae TIGR4

61.81

100

0.618
  radA Streptococcus mitis NCTC 12261

61.81

100

0.618
  radA Streptococcus mitis SK321

61.81

100

0.618

Multiple sequence alignment