Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACK25W_RS11300 Genome accession   NZ_CP179722
Coordinates   2224399..2225169 (-) Length   256 a.a.
NCBI ID   WP_034661344.1    Uniprot ID   -
Organism   Bacillus pumilus strain FJAT-56819     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2219399..2230169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS11280 (ACK25W_11280) - 2219489..2220310 (-) 822 WP_106071619.1 pyruvate, water dikinase regulatory protein -
  ACK25W_RS11285 (ACK25W_11285) - 2220336..2220974 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  ACK25W_RS11290 (ACK25W_11290) glyS 2221141..2223186 (-) 2046 WP_106039267.1 glycine--tRNA ligase subunit beta -
  ACK25W_RS11295 (ACK25W_11295) glyQ 2223179..2224066 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  ACK25W_RS11300 (ACK25W_11300) recO 2224399..2225169 (-) 771 WP_034661344.1 DNA repair protein RecO Machinery gene
  ACK25W_RS11305 (ACK25W_11305) - 2225205..2225348 (-) 144 WP_003217288.1 YqzL family protein -
  ACK25W_RS11310 (ACK25W_11310) era 2225497..2226402 (-) 906 WP_060596663.1 GTPase Era -
  ACK25W_RS11315 (ACK25W_11315) - 2226395..2226793 (-) 399 WP_034661347.1 cytidine deaminase -
  ACK25W_RS11320 (ACK25W_11320) - 2226907..2227293 (-) 387 WP_060596662.1 diacylglycerol kinase family protein -
  ACK25W_RS11325 (ACK25W_11325) ybeY 2227271..2227750 (-) 480 WP_012010615.1 rRNA maturation RNase YbeY -
  ACK25W_RS11330 (ACK25W_11330) - 2227751..2229862 (-) 2112 WP_374955736.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29244.92 Da        Isoelectric Point: 8.4348

>NTDB_id=1092859 ACK25W_RS11300 WP_034661344.1 2224399..2225169(-) (recO) [Bacillus pumilus strain FJAT-56819]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=1092859 ACK25W_RS11300 WP_034661344.1 2224399..2225169(-) (recO) [Bacillus pumilus strain FJAT-56819]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGCGTGATGGCAAGAGGTGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTTCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTTTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTAATGGGAATGAAGCCTGAACTTGATCAATGTGTCCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCTATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.781

100

0.758

Multiple sequence alignment