Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   ACK25W_RS10770 Genome accession   NZ_CP179722
Coordinates   2133946..2134749 (-) Length   267 a.a.
NCBI ID   WP_012010520.1    Uniprot ID   W6IDW7
Organism   Bacillus pumilus strain FJAT-56819     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2114434..2133810 2133946..2134749 flank 136


Gene organization within MGE regions


Location: 2114434..2134749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS10660 (ACK25W_10660) - 2114434..2114670 (+) 237 WP_034661240.1 DUF2627 domain-containing protein -
  ACK25W_RS10665 (ACK25W_10665) - 2114705..2115442 (-) 738 WP_066030681.1 glycerophosphodiester phosphodiesterase -
  ACK25W_RS10670 (ACK25W_10670) - 2115669..2116769 (-) 1101 WP_066030680.1 Rap family tetratricopeptide repeat protein -
  ACK25W_RS10675 (ACK25W_10675) - 2117876..2120665 (-) 2790 WP_414051588.1 phage tail tape measure protein -
  ACK25W_RS10680 (ACK25W_10680) - 2120662..2120889 (-) 228 WP_414051589.1 hypothetical protein -
  ACK25W_RS10685 (ACK25W_10685) - 2120965..2121279 (-) 315 WP_414051590.1 hypothetical protein -
  ACK25W_RS10690 (ACK25W_10690) - 2121512..2122222 (-) 711 WP_106029209.1 hypothetical protein -
  ACK25W_RS10695 (ACK25W_10695) - 2122245..2122910 (-) 666 WP_414051591.1 hypothetical protein -
  ACK25W_RS10700 (ACK25W_10700) - 2122922..2123398 (-) 477 WP_106029211.1 hypothetical protein -
  ACK25W_RS10705 (ACK25W_10705) - 2123428..2123736 (-) 309 WP_106029212.1 helix-turn-helix domain-containing protein -
  ACK25W_RS10710 (ACK25W_10710) - 2123848..2124348 (-) 501 WP_223251266.1 hypothetical protein -
  ACK25W_RS10715 (ACK25W_10715) - 2124363..2125559 (-) 1197 WP_223251267.1 hypothetical protein -
  ACK25W_RS10720 (ACK25W_10720) - 2125784..2126014 (-) 231 WP_106029214.1 helix-turn-helix domain-containing protein -
  ACK25W_RS10725 (ACK25W_10725) - 2126263..2126799 (-) 537 WP_106029215.1 hypothetical protein -
  ACK25W_RS10730 (ACK25W_10730) - 2126796..2127143 (-) 348 WP_106029216.1 hypothetical protein -
  ACK25W_RS10735 (ACK25W_10735) - 2127255..2127467 (+) 213 WP_106029217.1 helix-turn-helix domain-containing protein -
  ACK25W_RS10740 (ACK25W_10740) - 2127493..2128245 (+) 753 WP_106029218.1 hypothetical protein -
  ACK25W_RS10745 (ACK25W_10745) - 2128575..2128871 (-) 297 WP_106029219.1 YjcQ family protein -
  ACK25W_RS10750 (ACK25W_10750) - 2129033..2129587 (+) 555 WP_106029220.1 hypothetical protein -
  ACK25W_RS10755 (ACK25W_10755) - 2131410..2131946 (-) 537 WP_414051592.1 site-specific integrase -
  ACK25W_RS10760 (ACK25W_10760) - 2131949..2132677 (-) 729 WP_414051593.1 helix-turn-helix domain-containing protein -
  ACK25W_RS10765 (ACK25W_10765) - 2132839..2133810 (-) 972 WP_414051594.1 tyrosine-type recombinase/integrase -
  ACK25W_RS10770 (ACK25W_10770) spo0A 2133946..2134749 (-) 804 WP_012010520.1 sporulation transcription factor Spo0A Regulator

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29918.44 Da        Isoelectric Point: 6.3913

>NTDB_id=1092854 ACK25W_RS10770 WP_012010520.1 2133946..2134749(-) (spo0A) [Bacillus pumilus strain FJAT-56819]
MEKIKVCVADDNRELVGLLTEYIEGQEDMEVLGVAYNGQECLTLFKDKEPDVLLLDIIMPHLDGLAVLERLRENNDMTKQ
PSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNGTQVTHRSSSIQNSVLRNKPEPKRKNLDASITTI
IHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
SMSKAKPTNSEFIAMVADRLRLEHRAS

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1092854 ACK25W_RS10770 WP_012010520.1 2133946..2134749(-) (spo0A) [Bacillus pumilus strain FJAT-56819]
GTGGAGAAAATTAAAGTGTGTGTAGCTGATGACAATCGAGAGCTTGTTGGCCTTTTGACAGAGTATATTGAGGGACAGGA
AGATATGGAAGTACTTGGCGTGGCGTATAATGGTCAGGAATGTCTGACATTATTTAAAGATAAAGAGCCCGACGTTCTCC
TCTTAGATATTATCATGCCTCATTTAGACGGTCTTGCTGTACTGGAACGCCTCCGTGAAAACAATGACATGACCAAACAG
CCAAGTGTGATTATGCTAACGGCGTTTGGACAAGAAGATGTCACGAAAAAAGCAGTTGATTTGGGCGCATCCTATTTCAT
CTTAAAGCCATTTGATATGGAAAACCTAGTCGGTCATATTCGTCAAGTCAGTGGAAACGGCACACAAGTCACACATCGAT
CTTCATCTATCCAAAACAGTGTCCTTCGGAACAAACCTGAACCGAAACGTAAAAACTTAGATGCGAGCATTACGACGATT
ATTCATGAGATTGGCGTACCCGCTCATATTAAAGGATATTTATACTTAAGAGAAGCGATTTCGATGGTGTACAATGACAT
TGAGCTTCTCGGAAGCATCACAAAAGTGTTGTACCCAGACATCGCGAAGAAATTTAACACGACTGCCAGCAGGGTAGAAC
GAGCGATTCGTCATGCGATTGAAGTTGCTTGGAGCAGAGGAAACATCGATTCCATTTCCTCACTCTTTGGTTATACAGTC
AGCATGTCTAAAGCGAAACCAACCAATTCTGAATTTATTGCTATGGTGGCTGATCGTCTGCGCTTAGAGCATAGAGCTAG
CTGA

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(157-260)

Response regulator receiver domain (Response_reg)

(6-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W6IDW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

88.433

100

0.888

Multiple sequence alignment