Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACK25W_RS01820 Genome accession   NZ_CP179722
Coordinates   336454..336882 (-) Length   142 a.a.
NCBI ID   WP_060595509.1    Uniprot ID   -
Organism   Bacillus pumilus strain FJAT-56819     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 331454..341882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS01790 (ACK25W_01790) - 331612..331941 (+) 330 WP_106031600.1 YckD family protein -
  ACK25W_RS01795 (ACK25W_01795) - 331991..332755 (-) 765 WP_060595506.1 MBL fold metallo-hydrolase -
  ACK25W_RS01800 (ACK25W_01800) - 332870..333286 (+) 417 WP_034664465.1 Lrp/AsnC family transcriptional regulator -
  ACK25W_RS01805 (ACK25W_01805) - 333315..334121 (+) 807 WP_095285948.1 aminoglycoside N(3)-acetyltransferase -
  ACK25W_RS01810 (ACK25W_01810) - 334123..334509 (-) 387 WP_066031345.1 competence protein ComJ -
  ACK25W_RS01815 (ACK25W_01815) - 334630..336327 (-) 1698 WP_106037078.1 methyl-accepting chemotaxis protein -
  ACK25W_RS01820 (ACK25W_01820) nucA/comI 336454..336882 (-) 429 WP_060595509.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACK25W_RS01825 (ACK25W_01825) - 337250..339319 (+) 2070 WP_106037079.1 hydantoinase/oxoprolinase family protein -
  ACK25W_RS01830 (ACK25W_01830) - 339309..341294 (+) 1986 WP_106037080.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15916.82 Da        Isoelectric Point: 5.5444

>NTDB_id=1092826 ACK25W_RS01820 WP_060595509.1 336454..336882(-) (nucA/comI) [Bacillus pumilus strain FJAT-56819]
MKLLKIILLLLLIVVGVTTGYIQLEQSKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSVCTIDRKHSDEQRDQS
LHGIPTKRGYDRDEWPMAMCKEGGTGASVKYISPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1092826 ACK25W_RS01820 WP_060595509.1 336454..336882(-) (nucA/comI) [Bacillus pumilus strain FJAT-56819]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTACTAATCGTCGTTGGTGTAACAACAGGCTATATTCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTATGACAAAACCATTCACTTCCCATCAGATCGTTACCCAGAAACCGCGAAGCACA
TAGAAGAGGCAATTGATGAAGGCCATTCATCCGTATGTACGATTGACCGTAAACATAGCGATGAACAGAGAGACCAATCA
CTTCATGGCATCCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCGATGGCGATGTGTAAGGAAGGAGGAACAGGAGC
TTCTGTCAAATATATCAGTCCTTCAGATAATAGGGGAGCAGGCTCATGGGTCGGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATACAATTTATCATCGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.116

85.211

0.563

Multiple sequence alignment