Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACLUPT_RS03510 Genome accession   NZ_CP179718
Coordinates   742002..743603 (+) Length   533 a.a.
NCBI ID   WP_237678337.1    Uniprot ID   -
Organism   Variovorax sp. SCN45     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 737002..748603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUPT_RS03485 (ACLUPT_03485) argS 737096..738781 (+) 1686 WP_237678332.1 arginine--tRNA ligase -
  ACLUPT_RS03490 (ACLUPT_03490) - 738793..739557 (+) 765 WP_237678333.1 SPOR domain-containing protein -
  ACLUPT_RS03495 (ACLUPT_03495) - 739710..740357 (+) 648 WP_237678334.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACLUPT_RS03500 (ACLUPT_03500) lptA 740483..741193 (+) 711 WP_237678335.1 lipopolysaccharide transport periplasmic protein LptA -
  ACLUPT_RS03505 (ACLUPT_03505) lptB 741190..741984 (+) 795 WP_237678336.1 LPS export ABC transporter ATP-binding protein -
  ACLUPT_RS03510 (ACLUPT_03510) rpoN/rpoN1 742002..743603 (+) 1602 WP_237678337.1 RNA polymerase factor sigma-54 Machinery gene
  ACLUPT_RS03515 (ACLUPT_03515) - 743624..745303 (+) 1680 WP_414080185.1 phospholipase D family protein -
  ACLUPT_RS03520 (ACLUPT_03520) - 745323..745967 (-) 645 WP_237678338.1 LysE family translocator -
  ACLUPT_RS03525 (ACLUPT_03525) - 746113..746373 (+) 261 WP_237678339.1 hypothetical protein -
  ACLUPT_RS03530 (ACLUPT_03530) - 746407..747042 (-) 636 WP_237678340.1 YceI family protein -
  ACLUPT_RS03535 (ACLUPT_03535) - 747101..747682 (-) 582 WP_237678341.1 YceI family protein -
  ACLUPT_RS03540 (ACLUPT_03540) - 747679..748272 (-) 594 WP_237678342.1 cytochrome b -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 58898.14 Da        Isoelectric Point: 4.6069

>NTDB_id=1092807 ACLUPT_RS03510 WP_237678337.1 742002..743603(+) (rpoN/rpoN1) [Variovorax sp. SCN45]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAVDTPVQRDEVAEAGEGD
FTPPSASTSTASTETSTTAADSDGPAAEIAEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALSLRLSENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDL
GECLSIQLRALAAEDEDGEEEDRAQVYKTAIAICKQPMELLARRDFKRLATLTRSNEELVRLALQVIARLEPKPGRRFVD
VERNIVIPDVIVTKTGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRV
SNAIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRAL
IKQFVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=1092807 ACLUPT_RS03510 WP_237678337.1 742002..743603(+) (rpoN/rpoN1) [Variovorax sp. SCN45]
ATGAAGCAGGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCACTCACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTTCA
ACTTTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTCGACGAGAACCCGTTCCTCGAGCGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTCGATGCCGTCGACACGCCGGTCCAGCGCGATGAGGTGGCCGAAGCCGGCGAGGGCGAC
TTCACCCCGCCGTCGGCCAGCACCTCCACCGCCTCGACCGAGACCAGTACCACCGCGGCCGATTCCGACGGCCCCGCCGC
CGAGATCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAGTGGGGCAGCG
ATGCCCCCGCTCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGATGCCACCGAGCTCGCGCGCAGCCAGGAATCGCTG
CAGTCGTTCCTGCACCGCCAAGCGCTGAGCCTGCGCCTGAGCGAAAACGACAGCGCCGCGCTGCGCTTCCTGATCGAGTC
GCTGAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCGCTGGCCTCGGGCCTGGCCGGCGACGACAACGACCAGTTCG
ACGACCTCGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGACCTG
GGCGAATGCCTGAGCATCCAGCTGCGCGCGCTGGCCGCCGAGGACGAAGATGGCGAAGAAGAAGACCGCGCGCAGGTCTA
CAAGACCGCCATCGCCATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACCC
GCAGCAACGAAGAGCTGGTGCGCCTGGCGCTGCAGGTCATCGCGCGGCTGGAGCCCAAGCCGGGACGGCGTTTCGTCGAT
GTCGAACGCAACATCGTCATTCCCGACGTGATCGTCACCAAGACCGGCCGCGGCACCAACGTCAAGTTCCGCGTCATGCT
GAATCCCGAAGTCATGCCACGCCTGCGAGTGCACGACATCTATGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCC
AGGCGCTGTCGCAGCGGCTTCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTG
AGCAACGCCATCGTCGAGCGCCAGAAGAGCTACTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGAGAT
CGCCGACGAACTGGGCCTGCACGAATCCACCATTTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCGTTCGGCACGG
TCGAGCTGAAGTATTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTG
ATCAAGCAGTTCGTGAGTTCGGAGAGCATCAAGAAGCCGCTGTCGGACAGCCAGATTTCCGAGATGCTGAAGGAGCAGGG
CATTGAGTGCGCACGCCGCACCGTGGCCAAGTACCGCGAGGCGCTGCGTATCGCGCCCGCCAATCTGCGCAAGGCACTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-361)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.024

100

0.514

Multiple sequence alignment