Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACME8E_RS00145 Genome accession   NZ_CP178917
Coordinates   35217..35654 (-) Length   145 a.a.
NCBI ID   WP_165079631.1    Uniprot ID   -
Organism   Paenibacillus polymyxa strain AF2927     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 30217..40654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACME8E_RS00130 - 31513..33138 (-) 1626 WP_165079632.1 response regulator transcription factor -
  ACME8E_RS00135 - 33113..34933 (-) 1821 WP_058712101.1 sensor histidine kinase -
  ACME8E_RS00140 - 35048..35224 (+) 177 WP_013371919.1 hypothetical protein -
  ACME8E_RS00145 nucA/comI 35217..35654 (-) 438 WP_165079631.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACME8E_RS00150 - 35796..35990 (+) 195 WP_013371921.1 hypothetical protein -
  ACME8E_RS00155 - 36376..36678 (+) 303 WP_013371923.1 hypothetical protein -
  ACME8E_RS00160 - 36751..38637 (-) 1887 WP_129549586.1 Ig-like domain-containing protein -
  ACME8E_RS00165 - 38758..39180 (-) 423 WP_013371925.1 hypothetical protein -
  ACME8E_RS00170 - 39197..39865 (-) 669 WP_013371926.1 uracil-DNA glycosylase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15992.20 Da        Isoelectric Point: 9.4379

>NTDB_id=1092447 ACME8E_RS00145 WP_165079631.1 35217..35654(-) (nucA/comI) [Paenibacillus polymyxa strain AF2927]
MKRKLLSFIALVLLAVGAYWVEGDNLFTKITGENPPSSAQVTLQFPSGRYPETAQHIKEAIQAGKSPVCTIDREGAEQNR
KHSLAGVPTRKGFDRDEWPMAMCSEGGKGANVKYIAPKDNRGAGSWVSHQLDQYEDGTRVKFVIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1092447 ACME8E_RS00145 WP_165079631.1 35217..35654(-) (nucA/comI) [Paenibacillus polymyxa strain AF2927]
TTGAAGAGAAAGTTACTAAGTTTTATAGCCTTAGTGCTGCTAGCTGTGGGCGCGTACTGGGTTGAAGGAGACAATCTTTT
TACAAAAATAACAGGAGAAAACCCGCCTTCATCAGCTCAGGTAACGCTGCAATTTCCGTCAGGTCGTTATCCTGAAACGG
CGCAGCATATTAAGGAAGCCATTCAAGCGGGAAAATCACCAGTATGCACAATTGACCGGGAAGGAGCCGAGCAAAATCGT
AAGCATTCGCTTGCGGGTGTTCCTACTCGTAAAGGGTTTGATCGTGATGAATGGCCGATGGCTATGTGCTCAGAAGGGGG
AAAGGGTGCAAATGTCAAATACATAGCTCCTAAGGATAACCGTGGGGCAGGATCATGGGTCAGTCACCAGTTAGATCAGT
ATGAGGATGGAACTCGTGTAAAATTCGTTATTAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.308

71.724

0.483

Multiple sequence alignment