Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACL6EO_RS00550 Genome accession   NZ_CP178718
Coordinates   97373..98752 (+) Length   459 a.a.
NCBI ID   WP_017419468.1    Uniprot ID   A0AAP3YJ37
Organism   Bacillus velezensis strain SY1154     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92373..103752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6EO_RS00530 (ACL6EO_00530) ctsR 92725..93189 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  ACL6EO_RS00535 (ACL6EO_00535) - 93203..93760 (+) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  ACL6EO_RS00540 (ACL6EO_00540) - 93760..94845 (+) 1086 WP_413384634.1 protein arginine kinase -
  ACL6EO_RS00545 (ACL6EO_00545) clpC 94847..97279 (+) 2433 WP_007410388.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  ACL6EO_RS00550 (ACL6EO_00550) radA 97373..98752 (+) 1380 WP_017419468.1 DNA repair protein RadA Machinery gene
  ACL6EO_RS00555 (ACL6EO_00555) disA 98756..99838 (+) 1083 WP_003156401.1 DNA integrity scanning diadenylate cyclase DisA -
  ACL6EO_RS00560 (ACL6EO_00560) - 99952..101052 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  ACL6EO_RS00565 (ACL6EO_00565) ispD 101065..101763 (+) 699 WP_017419469.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACL6EO_RS00570 (ACL6EO_00570) ispF 101756..102232 (+) 477 WP_413384639.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49576.03 Da        Isoelectric Point: 7.1760

>NTDB_id=1090891 ACL6EO_RS00550 WP_017419468.1 97373..98752(+) (radA) [Bacillus velezensis strain SY1154]
MVKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1090891 ACL6EO_RS00550 WP_017419468.1 97373..98752(+) (radA) [Bacillus velezensis strain SY1154]
ATGGTTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCGGAAGAACCCCGCGTTCACACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGCATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGTGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTGTACCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAGGTGCTGAACCCTTCTGAAATATTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTGTCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAACTTGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.231

99.782

0.93
  radA Streptococcus pneumoniae Rx1

63.576

98.693

0.627
  radA Streptococcus pneumoniae D39

63.576

98.693

0.627
  radA Streptococcus pneumoniae R6

63.576

98.693

0.627
  radA Streptococcus pneumoniae TIGR4

63.576

98.693

0.627
  radA Streptococcus mitis NCTC 12261

63.576

98.693

0.627
  radA Streptococcus mitis SK321

63.355

98.693

0.625

Multiple sequence alignment