Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACL6ER_RS12225 Genome accession   NZ_CP178717
Coordinates   2475043..2475453 (-) Length   136 a.a.
NCBI ID   WP_268332609.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain SY1167     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2470043..2480453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6ER_RS12195 (ACL6ER_12195) - 2470299..2470430 (-) 132 WP_268285145.1 hypothetical protein -
  ACL6ER_RS12200 (ACL6ER_12200) - 2470506..2471237 (-) 732 WP_225514188.1 SGNH/GDSL hydrolase family protein -
  ACL6ER_RS12205 (ACL6ER_12205) - 2471490..2472242 (-) 753 WP_268332606.1 N-acetylmuramoyl-L-alanine amidase -
  ACL6ER_RS12210 (ACL6ER_12210) - 2472437..2473063 (+) 627 WP_003237129.1 TVP38/TMEM64 family protein -
  ACL6ER_RS12215 (ACL6ER_12215) gnd 2473082..2473975 (-) 894 WP_268332608.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACL6ER_RS12220 (ACL6ER_12220) - 2474288..2475010 (+) 723 Protein_2399 DUF308 domain-containing protein -
  ACL6ER_RS12225 (ACL6ER_12225) nucA/comI 2475043..2475453 (-) 411 WP_268332609.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACL6ER_RS12230 (ACL6ER_12230) - 2475648..2475998 (+) 351 Protein_2401 sigma-70 family RNA polymerase sigma factor -
  ACL6ER_RS12235 (ACL6ER_12235) spoIVCA 2476026..2477486 (-) 1461 WP_413327294.1 site-specific DNA recombinase SpoIVCA -
  ACL6ER_RS12240 (ACL6ER_12240) - 2477444..2477622 (-) 179 Protein_2403 hypothetical protein -
  ACL6ER_RS12245 (ACL6ER_12245) - 2478154..2478702 (-) 549 WP_413324753.1 NADPH-dependent FMN reductase -
  ACL6ER_RS12250 (ACL6ER_12250) - 2478938..2479897 (-) 960 WP_413324755.1 NAD(P)H-dependent flavin oxidoreductase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15035.05 Da        Isoelectric Point: 5.7516

>NTDB_id=1090858 ACL6ER_RS12225 WP_268332609.1 2475043..2475453(-) (nucA/comI) [Bacillus inaquosorum strain SY1167]
MKKWMAGLLLAAVVFLCLVVPQPIKSASLYDKVLYFPQSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVRFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1090858 ACL6ER_RS12225 WP_268332609.1 2475043..2475453(-) (nucA/comI) [Bacillus inaquosorum strain SY1167]
ATGAAAAAATGGATGGCAGGCCTGCTTCTTGCTGCAGTAGTTTTTCTTTGTTTAGTGGTTCCGCAGCCGATCAAGAGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCAGTCTCGTTATCCGGAAACAGGGGATCATATTAGAGATGCGATAGCAG
AGGGACATTCTGATATTTGTACCATCGACAGGGATGGAGCGGACAAAAGGCGAGAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCAATGGCGGTTTGCGAGGAAGGCGGCGCAGGAGCTGACGTCCGATATGTGAC
GCCTTCTGATAATCGTGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGTGGTTATCCTGACGGCACCAGAGTGCGGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(39-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment