Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACL6ER_RS04880 Genome accession   NZ_CP178717
Coordinates   1017054..1020758 (+) Length   1234 a.a.
NCBI ID   WP_268332234.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain SY1167     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1012054..1025758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6ER_RS04865 (ACL6ER_04865) - 1012504..1012830 (-) 327 WP_044427326.1 four-helix bundle copper-binding protein -
  ACL6ER_RS04870 (ACL6ER_04870) - 1012947..1013384 (-) 438 WP_044427329.1 ferritin family protein -
  ACL6ER_RS04875 (ACL6ER_04875) addB 1013567..1017067 (+) 3501 WP_413326470.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACL6ER_RS04880 (ACL6ER_04880) addA 1017054..1020758 (+) 3705 WP_268332234.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACL6ER_RS04885 (ACL6ER_04885) sbcD 1020830..1022005 (+) 1176 WP_268282248.1 exonuclease subunit SbcD -
  ACL6ER_RS04890 (ACL6ER_04890) sbcC 1022002..1025394 (+) 3393 WP_268317000.1 exonuclease subunit SbcC -
  ACL6ER_RS04895 (ACL6ER_04895) hlpB 1025408..1025710 (+) 303 WP_044427342.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141194.40 Da        Isoelectric Point: 5.0448

>NTDB_id=1090825 ACL6ER_RS04880 WP_268332234.1 1017054..1020758(+) (addA) [Bacillus inaquosorum strain SY1167]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
HELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENDKGEQEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAIALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQATASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAENGAGAHADISGHPARFAVQMIHSYD
LLDDDLEERLEEKSERLEAIRRGEPVPGPFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVHKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTQVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1090825 ACL6ER_RS04880 WP_268332234.1 1017054..1020758(+) (addA) [Bacillus inaquosorum strain SY1167]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATTACCGCAGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTCCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGGTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGAGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCTCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCGGAAAAGAGCGCAATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTGCAGCGCGCG
CATGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAACGGCGCAAAAAAACTGCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCATTTGAAAAGCTTAGCCGACATGAAGCCTGTTATTGAAACACTCGTACA
GCTTGTCATTAGCTACGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCAGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAACGACAAAGGCGAACAGGAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGATGAATATCAGGATACCAACCTCGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAGGAGACCGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGTGGAGAAGGCACGGGACGGAAAATCGACCTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCATACCCGGACAATGATGAGACGGAAACAGAGCTGCTGCTGATCGACAACTCAG
AAGAGACGGATGCAGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGT
AAGTTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGTTACGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTCGCGATCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTACTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAT
ACAAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTTCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGACGGAACTTCAACATGATGGACCTGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTTTATTCATCCGCAATTGCGCATCAGCTATCCGACGCTTCCGCTCATTGCCATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCAACTGCGTCCCAAACTGACTGGCTTCTCCCGGA
ATTTGACCGCTATCAGGCAAAAACCTATTTAGATTTCATCGGGCCGGCTCTTGCCAGGCACAGAGATTTGGAGGCTTTGG
CTGAAAACGGTGCGGGAGCGCATGCTGATATATCAGGCCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCTATGAT
TTGTTGGATGATGATCTGGAAGAAAGATTGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCC
CGGCCCGTTTGCGTTTGATGAGAAAGCCCGGGAGCAGCTAAGCTGGACATATCCGCATCATGAGGTGACGCAAATTCGCA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCTCCCGTTAAACCGGCA
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAGGGCCTGACAGCGGCGGAAAAAGGAACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTCACACATGTGCCGACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTTCAATTTTTTCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTCAGTTTAGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGTTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCGGCCCCGATCTTGAAAAAACGATACGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACACAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACACTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.921

100

0.969

Multiple sequence alignment