Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACKUFW_RS01775 Genome accession   NZ_CP178610
Coordinates   327302..327739 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain GUMHT p116     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 322302..332739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUFW_RS01735 (ACKUFW_01735) - 322886..323014 (-) 129 Protein_301 hypothetical protein -
  ACKUFW_RS01740 (ACKUFW_01740) - 323159..324358 (+) 1200 WP_202735830.1 GTP-binding protein -
  ACKUFW_RS01745 (ACKUFW_01745) - 324423..325559 (+) 1137 WP_077721769.1 zinc-dependent alcohol dehydrogenase -
  ACKUFW_RS01750 (ACKUFW_01750) - 325574..326008 (+) 435 WP_021494203.1 RDD family protein -
  ACKUFW_RS01755 (ACKUFW_01755) - 326126..326401 (+) 276 WP_369719109.1 YckD family protein -
  ACKUFW_RS01760 (ACKUFW_01760) - 326505..326621 (+) 117 WP_194298544.1 family 1 glycosylhydrolase -
  ACKUFW_RS01765 (ACKUFW_01765) - 326620..326838 (+) 219 Protein_307 family 1 glycosylhydrolase -
  ACKUFW_RS01770 (ACKUFW_01770) nin/comJ 326881..327279 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  ACKUFW_RS01775 (ACKUFW_01775) nucA/comI 327302..327739 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACKUFW_RS01780 (ACKUFW_01780) hxlB 328098..328655 (-) 558 WP_202735831.1 6-phospho-3-hexuloisomerase -
  ACKUFW_RS01785 (ACKUFW_01785) hxlA 328652..329287 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  ACKUFW_RS01790 (ACKUFW_01790) - 329519..329881 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1090446 ACKUFW_RS01775 WP_003156588.1 327302..327739(-) (nucA/comI) [Bacillus velezensis strain GUMHT p116]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1090446 ACKUFW_RS01775 WP_003156588.1 327302..327739(-) (nucA/comI) [Bacillus velezensis strain GUMHT p116]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTCACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment