Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ACJGE3_RS12470 Genome accession   NZ_CP178562
Coordinates   2562350..2563495 (+) Length   381 a.a.
NCBI ID   WP_077722992.1    Uniprot ID   -
Organism   Bacillus velezensis strain JT-3     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2557350..2568495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJGE3_RS12450 (ACJGE3_12450) - 2557825..2558541 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  ACJGE3_RS12455 (ACJGE3_12455) narK 2558639..2559829 (-) 1191 WP_369614464.1 nitrate transporter NarK -
  ACJGE3_RS12460 (ACJGE3_12460) argS 2559963..2561633 (-) 1671 WP_043020956.1 arginine--tRNA ligase -
  ACJGE3_RS12465 (ACJGE3_12465) - 2561630..2562058 (-) 429 WP_003151069.1 DUF1934 domain-containing protein -
  ACJGE3_RS12470 (ACJGE3_12470) rapF 2562350..2563495 (+) 1146 WP_077722992.1 tetratricopeptide repeat protein Regulator
  ACJGE3_RS12475 (ACJGE3_12475) phrF 2563479..2563598 (+) 120 WP_069473622.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  ACJGE3_RS12480 (ACJGE3_12480) - 2563797..2564000 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  ACJGE3_RS12485 (ACJGE3_12485) - 2564014..2564217 (+) 204 WP_069473623.1 hypothetical protein -
  ACJGE3_RS12490 (ACJGE3_12490) - 2564624..2564905 (+) 282 WP_014306021.1 hypothetical protein -
  ACJGE3_RS12495 (ACJGE3_12495) - 2564949..2565461 (+) 513 WP_077722993.1 stage II sporulation protein M -
  ACJGE3_RS12500 (ACJGE3_12500) - 2566052..2566270 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  ACJGE3_RS12505 (ACJGE3_12505) speB 2566440..2567312 (-) 873 WP_003151054.1 agmatinase -
  ACJGE3_RS12510 (ACJGE3_12510) speE 2567372..2568202 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45541.70 Da        Isoelectric Point: 4.6057

>NTDB_id=1090186 ACJGE3_RS12470 WP_077722992.1 2562350..2563495(+) (rapF) [Bacillus velezensis strain JT-3]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1090186 ACJGE3_RS12470 WP_077722992.1 2562350..2563495(+) (rapF) [Bacillus velezensis strain JT-3]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGATTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCACCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567

Multiple sequence alignment