Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACL2DY_RS17740 Genome accession   NZ_CP178544
Coordinates   3839281..3840720 (-) Length   479 a.a.
NCBI ID   WP_290024401.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain JK     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3834281..3845720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL2DY_RS17710 (ACL2DY_17710) dagF 3834553..3835293 (+) 741 WP_413162909.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  ACL2DY_RS17715 (ACL2DY_17715) - 3835398..3837302 (+) 1905 WP_413162911.1 BglG family transcription antiterminator -
  ACL2DY_RS17720 (ACL2DY_17720) npr 3837321..3837593 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  ACL2DY_RS17725 (ACL2DY_17725) rapZ 3837614..3838480 (-) 867 WP_005314405.1 RNase adapter RapZ -
  ACL2DY_RS17730 (ACL2DY_17730) ptsN 3838523..3838969 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACL2DY_RS17735 (ACL2DY_17735) hpf 3838972..3839259 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  ACL2DY_RS17740 (ACL2DY_17740) rpoN/rpoN1 3839281..3840720 (-) 1440 WP_290024401.1 RNA polymerase factor sigma-54 Machinery gene
  ACL2DY_RS17745 (ACL2DY_17745) lptB 3840786..3841511 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  ACL2DY_RS17750 (ACL2DY_17750) lptA 3841515..3842042 (-) 528 WP_413166150.1 lipopolysaccharide transport periplasmic protein LptA -
  ACL2DY_RS17755 (ACL2DY_17755) lptC 3842029..3842589 (-) 561 WP_413162915.1 LPS export ABC transporter periplasmic protein LptC -
  ACL2DY_RS17760 (ACL2DY_17760) kdsC 3842586..3843140 (-) 555 WP_282683332.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACL2DY_RS17765 (ACL2DY_17765) - 3843140..3844135 (-) 996 WP_034524103.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACL2DY_RS17770 (ACL2DY_17770) mlaF 3844343..3845143 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53891.84 Da        Isoelectric Point: 4.3484

>NTDB_id=1090072 ACL2DY_RS17740 WP_290024401.1 3839281..3840720(-) (rpoN/rpoN1) [Aeromonas salmonicida strain JK]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1090072 ACL2DY_RS17740 WP_290024401.1 3839281..3840720(-) (rpoN/rpoN1) [Aeromonas salmonicida strain JK]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGAGG
TTCAGGAGACCAGCCCGGAAGAGCCCGTAGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GATGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCATCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCGGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCTGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTGATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAACTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCAGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.972

100

0.489

Multiple sequence alignment