Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACL6C3_RS30655 Genome accession   NZ_CP178543
Coordinates   7529594..7531597 (-) Length   667 a.a.
NCBI ID   WP_413163941.1    Uniprot ID   -
Organism   Capilliphycus salinus ALCB114379 voucher ALCB 114379     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7524594..7536597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6C3_RS30640 (ACL6C3_30640) - 7526201..7526833 (-) 633 WP_413163935.1 hypothetical protein -
  ACL6C3_RS30645 (ACL6C3_30645) - 7527021..7528154 (-) 1134 WP_413163937.1 type II secretion system F family protein -
  ACL6C3_RS30650 (ACL6C3_30650) - 7528287..7529390 (-) 1104 WP_413163939.1 type IV pilus twitching motility protein PilT -
  ACL6C3_RS30655 (ACL6C3_30655) pilB/pilB1 7529594..7531597 (-) 2004 WP_413163941.1 GspE/PulE family protein Machinery gene
  ACL6C3_RS30660 (ACL6C3_30660) - 7531667..7531915 (+) 249 WP_413163943.1 hypothetical protein -
  ACL6C3_RS30665 (ACL6C3_30665) grpE 7532125..7532886 (+) 762 WP_413163944.1 nucleotide exchange factor GrpE -
  ACL6C3_RS30670 (ACL6C3_30670) dnaK 7533127..7535211 (+) 2085 WP_413163945.1 molecular chaperone DnaK -
  ACL6C3_RS30675 (ACL6C3_30675) dnaJ 7535319..7536452 (+) 1134 WP_413163946.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74606.43 Da        Isoelectric Point: 5.2862

>NTDB_id=1090049 ACL6C3_RS30655 WP_413163941.1 7529594..7531597(-) (pilB/pilB1) [Capilliphycus salinus ALCB114379 voucher ALCB 114379]
MTNISQRRAIVATSTTPFGNKLIQSGYISPEDLQQALKTSKNGKSLTEVLEAVTGKPLPPELARQYKKQQLFEQKIIYGV
DAFDLEVEQVSVEQIEQMLDTLETTIETCKRHRFIPLQILNSEPQAALIAMVDPDNLSAENELKNQLLRPKGWVLKKKVI
TQGDYQQLISAYSEEDLKRKQVEAARREEEKLQFTSGDFEEMDLEDVTDEAGGVDLADEIEQAETAPVIKAVNVILAKAL
SEGVSDIHVEPQENEMRIRMRKDGVLQEYFKFPKKVVPAITSRFKILAELDIAERRQAQDGRIRRRFKGRTVDFRVNSLP
SRYGEKIVLRILDSSSTQLGLDKLITDPETLAMVRETAGRPFGLILVTGPTGSGKSTSLYSVLAERNDPAINISTAEDPI
EYALPGITQCQVIREKNLTFANILRAFLRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGV
EPFMVSAALLGILAQRLMRRVCTECRIPYTPTPQELGRYGLSASREVNVEFFKANTIAPDQRKAHAERGELCKKCGGLGY
KGRVGVYEFLKVTERLQTLITQGAPTEQIKEVAVEEGMKTLLAYSLQLVREGHTTFEEVERVTFTDSGLEAELKAKRKQG
LTCKGCQAELRPEWLDCPYCMTPRFTD

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=1090049 ACL6C3_RS30655 WP_413163941.1 7529594..7531597(-) (pilB/pilB1) [Capilliphycus salinus ALCB114379 voucher ALCB 114379]
ATGACTAATATATCTCAGCGACGCGCCATAGTTGCGACCAGCACCACTCCTTTCGGTAATAAGTTAATTCAATCGGGATA
CATCAGCCCGGAAGACCTTCAGCAAGCCCTGAAAACGAGCAAAAATGGGAAATCCCTTACTGAAGTCTTAGAAGCGGTGA
CGGGGAAACCTTTGCCGCCCGAACTCGCACGTCAGTATAAAAAACAACAGCTTTTTGAGCAAAAAATTATTTACGGTGTT
GATGCTTTCGATTTAGAGGTCGAACAGGTATCGGTTGAGCAAATTGAACAAATGCTTGACACCTTAGAAACCACGATTGA
AACCTGCAAACGCCATAGGTTTATCCCATTGCAAATTCTCAACAGCGAACCCCAGGCTGCTTTGATTGCAATGGTCGATC
CGGACAACCTCAGCGCCGAGAACGAACTCAAAAACCAACTGTTGCGTCCGAAAGGCTGGGTTCTCAAAAAGAAAGTCATC
ACCCAAGGCGACTACCAGCAGTTAATCTCAGCCTACAGCGAAGAAGATCTCAAACGCAAGCAGGTTGAAGCCGCCCGTCG
GGAAGAAGAAAAACTTCAGTTCACCTCGGGCGACTTCGAGGAAATGGACTTAGAAGATGTCACCGATGAAGCCGGTGGGG
TTGACCTAGCCGATGAAATCGAACAAGCCGAAACCGCCCCGGTAATTAAAGCGGTGAACGTGATTCTCGCCAAAGCCTTA
TCTGAGGGAGTCTCCGATATTCACGTCGAACCGCAAGAAAATGAGATGCGGATTCGGATGAGGAAAGACGGGGTTTTGCA
GGAATATTTCAAATTTCCCAAAAAAGTTGTTCCGGCAATTACCTCTCGCTTTAAAATTCTCGCCGAATTAGACATCGCCG
AACGCCGTCAAGCCCAAGACGGAAGAATTCGCCGCCGGTTCAAAGGCAGAACCGTAGACTTTCGGGTAAACAGCTTACCG
AGTCGCTACGGTGAAAAAATTGTACTGCGGATTCTCGACAGTTCCTCAACTCAACTCGGACTCGATAAACTGATTACCGA
TCCGGAAACCCTGGCGATGGTACGGGAAACCGCCGGCCGACCTTTTGGGTTAATCTTGGTAACAGGGCCGACGGGTTCGG
GTAAATCGACTTCTTTGTATTCGGTTCTCGCCGAACGCAATGACCCCGCCATTAACATCAGTACGGCCGAAGACCCGATT
GAATACGCTTTACCGGGGATTACTCAATGTCAGGTGATTCGCGAAAAAAACTTGACCTTCGCCAATATTCTCCGGGCTTT
CCTCAGACAAGACCCGGATGTGTTGCTGGTGGGAGAAACGCGGGACTTAGAAACGGCAAAAACGGCGATTGAAGCCGCTT
TGACCGGACACTTGGTTTTAACTACATTACACACCAACGACGCCGCCGGGGCGATCGCCCGTTTGGATGAAATGGGGGTT
GAACCGTTCATGGTGTCGGCGGCGTTACTGGGTATTCTCGCCCAACGTTTGATGCGTCGGGTGTGTACCGAATGCCGGAT
TCCCTATACACCGACCCCGCAAGAACTCGGACGTTATGGCTTGTCGGCTTCGCGAGAGGTGAATGTGGAGTTTTTCAAAG
CCAATACCATCGCCCCCGACCAACGCAAAGCCCACGCTGAACGGGGCGAACTCTGTAAAAAATGTGGCGGTTTGGGATAC
AAAGGTCGGGTCGGGGTTTACGAGTTCCTGAAAGTCACCGAACGCCTGCAAACGTTAATCACCCAAGGCGCCCCCACCGA
ACAAATTAAGGAAGTGGCGGTTGAGGAGGGGATGAAAACGTTACTGGCTTACAGTTTACAACTGGTCCGCGAAGGTCATA
CCACCTTTGAAGAAGTCGAACGGGTGACATTTACCGACTCGGGTTTAGAAGCGGAACTGAAGGCTAAACGCAAGCAGGGC
TTGACCTGTAAGGGTTGCCAAGCCGAACTTCGACCCGAATGGCTCGATTGTCCCTACTGTATGACTCCCCGGTTTACCGA
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(22-174)

Type II/IV secretion system protein (T2SSE)

(235-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.048

99.55

0.618

Multiple sequence alignment