Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACLZ2J_RS01990 Genome accession   NZ_CP178514
Coordinates   371696..372145 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis strain BS18-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366696..377145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLZ2J_RS01955 yckA 366847..367527 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  ACLZ2J_RS01960 yckB 367537..368400 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  ACLZ2J_RS01965 - 368562..368759 (+) 198 WP_119122841.1 hypothetical protein -
  ACLZ2J_RS01970 yckC 368778..369233 (+) 456 WP_003246356.1 RDD family protein -
  ACLZ2J_RS01975 yckD 369315..369647 (+) 333 WP_003246247.1 YckD family protein -
  ACLZ2J_RS01980 bglC 369801..371234 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  ACLZ2J_RS01985 nin/comJ 371271..371669 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  ACLZ2J_RS01990 nucA/comI 371696..372145 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  ACLZ2J_RS01995 tlpC 372313..374034 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  ACLZ2J_RS02000 hxlB 374145..374702 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  ACLZ2J_RS02005 hxlA 374708..375340 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  ACLZ2J_RS02010 hxlR 375573..375935 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=1089770 ACLZ2J_RS01990 WP_003246436.1 371696..372145(-) (nucA/comI) [Bacillus subtilis strain BS18-1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1089770 ACLZ2J_RS01990 WP_003246436.1 371696..372145(-) (nucA/comI) [Bacillus subtilis strain BS18-1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment