Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACLNGX_RS12255 Genome accession   NZ_CP178374
Coordinates   2583450..2584217 (-) Length   255 a.a.
NCBI ID   WP_166706133.1    Uniprot ID   -
Organism   Bacillus velezensis strain 2023     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2578450..2589217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLNGX_RS12235 (ACLNGX_12235) - 2578653..2579465 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  ACLNGX_RS12240 (ACLNGX_12240) ccpN 2579491..2580129 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  ACLNGX_RS12245 (ACLNGX_12245) glyS 2580229..2582268 (-) 2040 WP_166706135.1 glycine--tRNA ligase subunit beta -
  ACLNGX_RS12250 (ACLNGX_12250) glyQ 2582261..2583148 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  ACLNGX_RS12255 (ACLNGX_12255) recO 2583450..2584217 (-) 768 WP_166706133.1 DNA repair protein RecO Machinery gene
  ACLNGX_RS12260 (ACLNGX_12260) - 2584254..2584397 (-) 144 WP_007408286.1 YqzL family protein -
  ACLNGX_RS12265 (ACLNGX_12265) era 2584553..2585458 (-) 906 WP_007408285.1 GTPase Era -
  ACLNGX_RS12270 (ACLNGX_12270) - 2585439..2585849 (-) 411 WP_003152976.1 cytidine deaminase -
  ACLNGX_RS12275 (ACLNGX_12275) - 2585973..2586347 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  ACLNGX_RS12280 (ACLNGX_12280) ybeY 2586328..2586801 (-) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  ACLNGX_RS12285 (ACLNGX_12285) - 2586802..2588934 (-) 2133 WP_015387990.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29303.96 Da        Isoelectric Point: 8.3139

>NTDB_id=1089162 ACLNGX_RS12255 WP_166706133.1 2583450..2584217(-) (recO) [Bacillus velezensis strain 2023]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQMARLLRLFYYFDIGRLGSVSLKQETKNEIKHVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1089162 ACLNGX_RS12255 WP_166706133.1 2583450..2584217(-) (recO) [Bacillus velezensis strain 2023]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAATGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CGAAAAACGAAATTAAACATGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.627

100

0.886

Multiple sequence alignment