Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACLNGX_RS10655 Genome accession   NZ_CP178374
Coordinates   2252436..2253044 (+) Length   202 a.a.
NCBI ID   WP_003153521.1    Uniprot ID   -
Organism   Bacillus velezensis strain 2023     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2247436..2258044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLNGX_RS10615 (ACLNGX_10615) - 2248489..2248995 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  ACLNGX_RS10620 (ACLNGX_10620) - 2249134..2249547 (+) 414 WP_070082015.1 Hsp20/alpha crystallin family protein -
  ACLNGX_RS10625 (ACLNGX_10625) - 2249577..2250011 (-) 435 WP_101562217.1 YppG family protein -
  ACLNGX_RS10630 (ACLNGX_10630) - 2250198..2250380 (+) 183 WP_003153532.1 YppF family protein -
  ACLNGX_RS10635 (ACLNGX_10635) - 2250419..2250787 (-) 369 WP_003153529.1 YppE family protein -
  ACLNGX_RS10640 (ACLNGX_10640) - 2250833..2251078 (-) 246 WP_039063257.1 DUF5446 family protein -
  ACLNGX_RS10645 (ACLNGX_10645) sspM 2251284..2251388 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  ACLNGX_RS10650 (ACLNGX_10650) - 2251432..2252394 (-) 963 WP_198863772.1 DUF2515 domain-containing protein -
  ACLNGX_RS10655 (ACLNGX_10655) recU 2252436..2253044 (+) 609 WP_003153521.1 Holliday junction resolvase RecU Machinery gene
  ACLNGX_RS10660 (ACLNGX_10660) - 2253083..2255866 (+) 2784 WP_101562219.1 PBP1A family penicillin-binding protein -
  ACLNGX_RS10665 (ACLNGX_10665) - 2255944..2256435 (-) 492 WP_101562220.1 YpoC family protein -
  ACLNGX_RS10670 (ACLNGX_10670) nth 2256432..2257091 (-) 660 WP_003153517.1 endonuclease III -
  ACLNGX_RS10675 (ACLNGX_10675) dnaD 2257110..2257808 (-) 699 WP_003153516.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=1089151 ACLNGX_RS10655 WP_003153521.1 2252436..2253044(+) (recU) [Bacillus velezensis strain 2023]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=1089151 ACLNGX_RS10655 WP_003153521.1 2252436..2253044(+) (recU) [Bacillus velezensis strain 2023]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTCGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AAGAACGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.955

98.515

0.896

Multiple sequence alignment