Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACLNGX_RS01785 Genome accession   NZ_CP178374
Coordinates   329597..330034 (-) Length   145 a.a.
NCBI ID   WP_071392144.1    Uniprot ID   -
Organism   Bacillus velezensis strain 2023     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 324597..335034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLNGX_RS01745 (ACLNGX_01745) - 325191..325319 (-) 129 Protein_303 hypothetical protein -
  ACLNGX_RS01750 (ACLNGX_01750) - 325464..326666 (+) 1203 WP_166706416.1 GTP-binding protein -
  ACLNGX_RS01755 (ACLNGX_01755) - 326731..327867 (+) 1137 WP_063174920.1 zinc-dependent alcohol dehydrogenase -
  ACLNGX_RS01760 (ACLNGX_01760) - 327882..328316 (+) 435 WP_021494203.1 RDD family protein -
  ACLNGX_RS01765 (ACLNGX_01765) - 328433..328708 (+) 276 WP_373369498.1 YckD family protein -
  ACLNGX_RS01770 (ACLNGX_01770) - 328812..329003 (+) 192 WP_071392145.1 family 1 glycosylhydrolase -
  ACLNGX_RS01775 (ACLNGX_01775) - 328928..329157 (+) 230 Protein_309 family 1 glycosylhydrolase -
  ACLNGX_RS01780 (ACLNGX_01780) nin/comJ 329176..329574 (-) 399 WP_166706415.1 competence protein ComJ Regulator
  ACLNGX_RS01785 (ACLNGX_01785) nucA/comI 329597..330034 (-) 438 WP_071392144.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACLNGX_RS01790 (ACLNGX_01790) hxlB 330395..330952 (-) 558 WP_071392143.1 6-phospho-3-hexuloisomerase -
  ACLNGX_RS01795 (ACLNGX_01795) hxlA 330949..331584 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  ACLNGX_RS01800 (ACLNGX_01800) - 331816..332178 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16099.43 Da        Isoelectric Point: 9.4115

>NTDB_id=1089122 ACLNGX_RS01785 WP_071392144.1 329597..330034(-) (nucA/comI) [Bacillus velezensis strain 2023]
MKTLKALLLVLIIIAGAVAALIKGDLFSNNEQTVRKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1089122 ACLNGX_RS01785 WP_071392144.1 329597..330034(-) (nucA/comI) [Bacillus velezensis strain 2023]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAAATAACGAGCAGACCGTTCGGAAGAGTGATTACGATAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGG
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634

Multiple sequence alignment