Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLFAM_RS05020 Genome accession   NZ_CP178226
Coordinates   1010316..1011677 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain L-WY410     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1005316..1016677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLFAM_RS04985 (ACLFAM_04985) - 1005864..1006358 (+) 495 WP_012898594.1 GNAT family N-acetyltransferase -
  ACLFAM_RS04990 (ACLFAM_04990) - 1006450..1006773 (-) 324 WP_039116271.1 DUF1048 domain-containing protein -
  ACLFAM_RS04995 (ACLFAM_04995) - 1006760..1007107 (-) 348 WP_012898595.1 DUF1048 domain-containing protein -
  ACLFAM_RS05000 (ACLFAM_05000) - 1007094..1007456 (-) 363 WP_023188602.1 PadR family transcriptional regulator -
  ACLFAM_RS05005 (ACLFAM_05005) - 1007577..1008206 (-) 630 WP_023188601.1 hypothetical protein -
  ACLFAM_RS05010 (ACLFAM_05010) - 1008271..1009356 (-) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  ACLFAM_RS05015 (ACLFAM_05015) - 1009587..1010273 (+) 687 WP_052230935.1 YoaK family protein -
  ACLFAM_RS05020 (ACLFAM_05020) radA 1010316..1011677 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  ACLFAM_RS05025 (ACLFAM_05025) - 1011839..1012066 (-) 228 WP_023188599.1 heavy-metal-associated domain-containing protein -
  ACLFAM_RS05030 (ACLFAM_05030) - 1012365..1012622 (-) 258 WP_080668830.1 SIS domain-containing protein -
  ACLFAM_RS05035 (ACLFAM_05035) - 1012598..1012852 (-) 255 WP_039116277.1 SIS domain-containing protein -
  ACLFAM_RS05045 (ACLFAM_05045) rplQ 1013547..1013927 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  ACLFAM_RS05050 (ACLFAM_05050) - 1013946..1014884 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  ACLFAM_RS05055 (ACLFAM_05055) rpsK 1014932..1015315 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  ACLFAM_RS05060 (ACLFAM_05060) rpsM 1015336..1015701 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  ACLFAM_RS05065 (ACLFAM_05065) rpmJ 1015719..1015835 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  ACLFAM_RS05070 (ACLFAM_05070) infA 1015889..1016107 (-) 219 WP_003130554.1 translation initiation factor IF-1 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=1088551 ACLFAM_RS05020 WP_003130563.1 1010316..1011677(-) (radA) [Lactococcus lactis strain L-WY410]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1088551 ACLFAM_RS05020 WP_003130563.1 1010316..1011677(-) (radA) [Lactococcus lactis strain L-WY410]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCTTTGGTTCTGATTGGGGGAGATCCTGGGATAGGTAAATCAACTTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGGCCGAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTAACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCGATTG
TTTGTGCACTTGAGGGAACGCGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGACTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTGACAACTTTAACTGAATGTTTAAAACTGGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment