Detailed information NTG1088474

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACLD94_RS11825
• Genome accession: NZ_CP178220
• Coordinates: 2460547..2461161 (-)
• Length: 204 a.a.
• NCBI ID: WP_032873955.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JN-Y2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2455547..2466161

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLD94_RS11795 (ACLD94_11795)	rpsT	2455850..2456116 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACLD94_RS11800 (ACLD94_11800)	holA	2456133..2457174 (-)	1042	Protein_2279	DNA polymerase III subunit delta	-
ACLD94_RS11805 (ACLD94_11805)	-	2457214..2457365 (-)	152	Protein_2280	hypothetical protein	-
ACLD94_RS11810 (ACLD94_11810)	-	2457406..2457540 (+)	135	WP_003152870.1	YqzM family protein	-
ACLD94_RS11815 (ACLD94_11815)	comEC	2457559..2459910 (-)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLD94_RS11820 (ACLD94_11820)	-	2459911..2460480 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACLD94_RS11825 (ACLD94_11825)	comEA	2460547..2461161 (-)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLD94_RS11830 (ACLD94_11830)	comER	2461220..2462041 (+)	822	WP_012118027.1	late competence protein ComER	-
ACLD94_RS11835 (ACLD94_11835)	-	2462110..2462847 (-)	738	WP_032873951.1	class I SAM-dependent DNA methyltransferase	-
ACLD94_RS11840 (ACLD94_11840)	rsfS	2462844..2463200 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACLD94_RS11845 (ACLD94_11845)	yqeK	2463204..2463779 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACLD94_RS11850 (ACLD94_11850)	-	2463769..2464338 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACLD94_RS11855 (ACLD94_11855)	yhbY	2464349..2464639 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACLD94_RS11860 (ACLD94_11860)	aroE	2464633..2465475 (-)	843	WP_032873946.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22265.47 Da
• Isoelectric Point: 6.4940

>NTDB_id=1088474 ACLD94_RS11825 WP_032873955.1 2460547..2461161(-) (comEA) [Bacillus velezensis strain JN-Y2]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1088474 ACLD94_RS11825 WP_032873955.1 2460547..2461161(-) (comEA) [Bacillus velezensis strain JN-Y2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363