Detailed information NTG0108774

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: ABWW09_RS00095
• Genome accession: NZ_AP031332
• Coordinates: 18948..21614 (-)
• Length: 888 a.a.
• NCBI ID: WP_353300384.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain TF27
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13948..26614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABWW09_RS00075 (TthTF27_00120)	-	14794..15426 (-)	633	WP_014628814.1	histidine phosphatase family protein	-
ABWW09_RS00080 (TthTF27_00130)	purM	15423..16424 (-)	1002	WP_353300382.1	phosphoribosylformylglycinamidine cyclo-ligase	-
ABWW09_RS00085 (TthTF27_00140)	gatB	16430..17839 (-)	1410	WP_353300383.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
ABWW09_RS00090 (TthTF27_00150)	pilT	17850..18935 (-)	1086	WP_143584087.1	type IV pilus twitching motility protein PilT	Machinery gene
ABWW09_RS00095 (TthTF27_00160)	pilF	18948..21614 (-)	2667	WP_353300384.1	type IV pilus assembly ATPase PilB	Machinery gene
ABWW09_RS00100 (TthTF27_00170)	-	21611..22096 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
ABWW09_RS00105 (TthTF27_00180)	pgeF	22131..22859 (-)	729	WP_014628819.1	peptidoglycan editing factor PgeF	-
ABWW09_RS00110 (TthTF27_00190)	surE	22856..23611 (-)	756	WP_011173994.1	5'/3'-nucleotidase SurE	-
ABWW09_RS00115 (TthTF27_00200)	-	23670..23876 (-)	207	WP_014628821.1	cold-shock protein	-
ABWW09_RS00120 (TthTF27_00210)	-	24032..25321 (+)	1290	WP_014628822.1	ABC transporter substrate-binding protein	-
ABWW09_RS00125 (TthTF27_00220)	-	25373..26248 (+)	876	WP_244341037.1	sugar ABC transporter permease	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 97954.09 Da
• Isoelectric Point: 5.3965

>NTDB_id=108774 ABWW09_RS00095 WP_353300384.1 18948..21614(-) (pilF) [Thermus thermophilus strain TF27]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSIEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLMLGEFLLKKGLISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAAALL
LRSDALRYGAVPIGFQNGKVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPEKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRQGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEQDPP
DPGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLAYEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQEEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVIS
VVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLS
EAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=108774 ABWW09_RS00095 WP_353300384.1 18948..21614(-) (pilF) [Thermus thermophilus strain TF27]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAAAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCATCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTCTACGCCCTGGCCAAGCACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAAAAGGACCTCATGCTCGGGGAGTTCCTCCTGAAAAAGGGGCTGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CTTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCGCCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGAAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCATAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCCGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCCGAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCAGGGAGGCGCTA
AAGGAAGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCAGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGCAGGACCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAATATCCTGGCCCTGGACGACGTGCGCCTGGCCCTGAAGCGGAAGGGGTTGG
CCTACGAGGTGGCCCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCAAAAAGCAGGAGGAGGAGGCCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGCCCA
GAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACGACG
TCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATCTCC
GTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAACGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGC
CATCGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCT
CGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCCTAC
GGCATCTTCCTCATCACCGGGCCCACAGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCC
CGACAAAAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATTCCCGGGATCAACCAGACCCAGGTGAACCCGCAGG
CGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTTAACATCTCCGCCGCCCTCATCGGCGTCCTCTCCCAGC
GCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTAAGGCGCCTCGGGCTTTCC
GAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGG
AGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAG
GAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II/IV secretion system protein (T2SSE) (512-778)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	98.425	100	0.985
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.067	100	0.562