Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLH2T_RS08875 Genome accession   NZ_CP177348
Coordinates   1753369..1754739 (-) Length   456 a.a.
NCBI ID   WP_002832361.1    Uniprot ID   A0AAN5Y9S9
Organism   Pediococcus acidilactici strain GR-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1748369..1759739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH2T_RS08855 (ACLH2T_08855) - 1748511..1748924 (-) 414 WP_004165556.1 Mini-ribonuclease 3 -
  ACLH2T_RS08860 (ACLH2T_08860) cysS 1748917..1750335 (-) 1419 WP_024862956.1 cysteine--tRNA ligase -
  ACLH2T_RS08865 (ACLH2T_08865) gltX 1750492..1751979 (-) 1488 WP_002832359.1 glutamate--tRNA ligase -
  ACLH2T_RS08870 (ACLH2T_08870) - 1752105..1753253 (-) 1149 WP_002832360.1 PIN/TRAM domain-containing protein -
  ACLH2T_RS08875 (ACLH2T_08875) radA 1753369..1754739 (-) 1371 WP_002832361.1 DNA repair protein RadA Machinery gene
  ACLH2T_RS08880 (ACLH2T_08880) - 1754833..1755369 (-) 537 WP_024862955.1 dUTP diphosphatase -
  ACLH2T_RS08885 (ACLH2T_08885) - 1755498..1755821 (+) 324 WP_024862954.1 GNAT family N-acetyltransferase -
  ACLH2T_RS08890 (ACLH2T_08890) rpiA 1755832..1756518 (+) 687 WP_002832365.1 ribose-5-phosphate isomerase RpiA -
  ACLH2T_RS08895 (ACLH2T_08895) - 1756567..1757913 (+) 1347 WP_024862953.1 aminopeptidase C -
  ACLH2T_RS08900 (ACLH2T_08900) - 1758267..1758938 (-) 672 WP_024862952.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  ACLH2T_RS08905 (ACLH2T_08905) accA 1758962..1759729 (-) 768 WP_347920696.1 carboxyltransferase subunit alpha -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50252.79 Da        Isoelectric Point: 7.4153

>NTDB_id=1086740 ACLH2T_RS08875 WP_002832361.1 1753369..1754739(-) (radA) [Pediococcus acidilactici strain GR-5]
MAKVKTQFVCNNCGYASPKFLGRCPNCGKWNTLVEERMSDPKAERKSRVSFDGKHTQPQLISDVAMHEEPRVKTGMEELN
RVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAETQRKVLYVSGEESASQIKMRAERLKVNSERFYLYPETDMSSVRA
VIEELHPEYVIIDSVQTMQEPDIESAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLKDASGSAIVVSMEGTRPILVEIQALITPTIFG
NAQRTASGLDRNRVSLIMAVLEKRAKLTLQNQDAYLKAAGGVKLDEPAIDLALAISIVSSFKNQGTRPTDSFVGEIGLTG
EIRRVNRIEQRVAEAQKLGFKRILIPKNNLKGWQPPEGIEVVGVATIADAIRRAFN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1086740 ACLH2T_RS08875 WP_002832361.1 1753369..1754739(-) (radA) [Pediococcus acidilactici strain GR-5]
ATGGCAAAAGTTAAGACCCAGTTTGTATGCAATAATTGCGGATACGCCTCCCCTAAATTTTTAGGACGCTGTCCCAATTG
TGGTAAGTGGAACACTTTAGTTGAAGAACGGATGAGCGATCCAAAGGCTGAACGAAAAAGTCGGGTGAGCTTTGACGGCA
AACATACGCAACCACAGCTGATTTCGGACGTCGCGATGCACGAGGAACCCCGGGTAAAAACCGGGATGGAAGAACTTAAC
CGCGTGCTTGGTGGCGGGGTGGTTGACGGCTCCCTAGTCTTAATTGGTGGGGATCCTGGAATCGGAAAATCAACCCTGTT
GTTACAGCTATCAGGACAGTTGGCAGAGACGCAACGCAAGGTACTTTACGTTTCTGGTGAAGAAAGTGCTTCCCAGATTA
AGATGCGTGCCGAACGATTGAAAGTCAATAGTGAACGCTTCTATTTGTATCCAGAGACCGATATGTCTAGCGTGCGGGCG
GTGATTGAGGAGTTACACCCCGAATACGTGATCATTGATTCGGTTCAGACCATGCAGGAACCGGATATTGAATCGGCGGT
GGGGAGCGTTTCTCAAATTCGAGAAATTACCGCAGAATTAATGCAAATCGCGAAAACCAATAACATTACCATCTTCATCG
TTGGTCACGTGACCAAGGGCGGCGCAATTGCGGGGCCGAAGATTTTGGAACACATGGTGGATACGGTGCTTTACTTTGAA
GGTGATCTGCACCACACTTACCGGATCCTGCGAGCGGTGAAGAACCGGTTTGGTTCCACGAACGAATTAGGAATCTTTGA
GATGCGCGAAGAAGGGTTACGCGAGGTGGCTAACCCGTCAGAAATTTTCTTAGAAGAGCGTTTAAAAGATGCTTCGGGAT
CGGCAATTGTAGTTTCAATGGAAGGAACTCGGCCGATTTTAGTTGAAATACAAGCCTTGATTACGCCCACCATCTTCGGC
AATGCGCAGCGCACGGCGAGCGGGTTGGACCGCAACCGGGTTTCGCTGATCATGGCGGTGCTCGAAAAACGGGCAAAGCT
TACTTTGCAAAACCAAGACGCCTATTTAAAGGCGGCGGGCGGCGTTAAGCTTGACGAACCCGCAATTGACTTAGCGTTGG
CGATTAGTATTGTTTCAAGTTTTAAAAATCAGGGAACCCGGCCGACCGATAGTTTTGTTGGTGAAATCGGCTTAACTGGT
GAAATTCGGCGGGTTAATCGAATTGAACAACGGGTCGCTGAAGCACAAAAGCTTGGTTTTAAGCGGATTTTGATTCCGAA
GAATAATTTGAAGGGGTGGCAGCCCCCCGAAGGAATTGAAGTTGTAGGGGTAGCTACGATTGCCGACGCGATTCGGCGGG
CATTTAATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.033

99.781

0.669
  radA Streptococcus mitis NCTC 12261

66.813

99.781

0.667
  radA Streptococcus pneumoniae Rx1

66.813

99.781

0.667
  radA Streptococcus pneumoniae TIGR4

66.813

99.781

0.667
  radA Streptococcus pneumoniae D39

66.813

99.781

0.667
  radA Streptococcus pneumoniae R6

66.813

99.781

0.667
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654

Multiple sequence alignment