Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACLF6N_RS18875 Genome accession   NZ_CP177279
Coordinates   3511246..3512637 (-) Length   463 a.a.
NCBI ID   WP_412052475.1    Uniprot ID   -
Organism   Bacillus subtilis strain KFRI-P74     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3506246..3517637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6N_RS18840 (ACLF6N_18840) flgL 3506418..3507314 (-) 897 WP_014481046.1 flagellar hook-associated protein FlgL -
  ACLF6N_RS18845 (ACLF6N_18845) flgK 3507325..3508848 (-) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  ACLF6N_RS18850 (ACLF6N_18850) flgN 3508867..3509349 (-) 483 WP_014481048.1 flagellar protein FlgN -
  ACLF6N_RS18855 (ACLF6N_18855) flgM 3509365..3509631 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACLF6N_RS18860 (ACLF6N_18860) yvyF 3509712..3510131 (-) 420 WP_038429728.1 TIGR03826 family flagellar region protein -
  ACLF6N_RS18865 (ACLF6N_18865) comFC 3510204..3510926 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  ACLF6N_RS18870 (ACLF6N_18870) comFB 3510890..3511186 (-) 297 WP_003227989.1 late competence protein ComFB -
  ACLF6N_RS18875 (ACLF6N_18875) comFA 3511246..3512637 (-) 1392 WP_412052475.1 ATP-dependent helicase ComFA Machinery gene
  ACLF6N_RS18880 (ACLF6N_18880) fakBA 3512743..3513588 (-) 846 WP_014481053.1 DegV family protein -
  ACLF6N_RS18885 (ACLF6N_18885) degU 3513686..3514375 (-) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  ACLF6N_RS18890 (ACLF6N_18890) degS 3514458..3515615 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  ACLF6N_RS18895 (ACLF6N_18895) - 3515832..3516485 (+) 654 WP_014481055.1 YigZ family protein -
  ACLF6N_RS18900 (ACLF6N_18900) - 3516485..3517477 (+) 993 WP_014481056.1 LCP family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52569.62 Da        Isoelectric Point: 9.8572

>NTDB_id=1086574 ACLF6N_RS18875 WP_412052475.1 3511246..3512637(-) (comFA) [Bacillus subtilis strain KFRI-P74]
MNVPVEKNISFSKELRQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=1086574 ACLF6N_RS18875 WP_412052475.1 3511246..3512637(-) (comFA) [Bacillus subtilis strain KFRI-P74]
GTGAATGTGCCAGTTGAAAAAAACATTTCCTTTTCAAAAGAATTGCGGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

97.84

100

0.978

Multiple sequence alignment