Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | ACLF6N_RS13660 | Genome accession | NZ_CP177279 |
| Coordinates | 2509602..2510012 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain KFRI-P74 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2504967..2532889 | 2509602..2510012 | within | 0 |
Gene organization within MGE regions
Location: 2504967..2532889
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACLF6N_RS13635 (ACLF6N_13635) | yqeF | 2505134..2505865 (-) | 732 | WP_412052439.1 | SGNH/GDSL hydrolase family protein | - |
| ACLF6N_RS13640 (ACLF6N_13640) | cwlH | 2506117..2506869 (-) | 753 | WP_021480067.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| ACLF6N_RS13645 (ACLF6N_13645) | yqeD | 2507056..2507682 (+) | 627 | WP_046160610.1 | TVP38/TMEM64 family protein | - |
| ACLF6N_RS13650 (ACLF6N_13650) | gnd | 2507702..2508595 (-) | 894 | WP_046160611.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| ACLF6N_RS13655 (ACLF6N_13655) | yqeB | 2508847..2509569 (+) | 723 | WP_014480321.1 | hypothetical protein | - |
| ACLF6N_RS13660 (ACLF6N_13660) | nucA/comI | 2509602..2510012 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| ACLF6N_RS13665 (ACLF6N_13665) | sigK | 2510208..2510936 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| ACLF6N_RS13670 (ACLF6N_13670) | - | 2510936..2511034 (+) | 99 | WP_031600702.1 | hypothetical protein | - |
| ACLF6N_RS13675 (ACLF6N_13675) | - | 2511031..2511243 (-) | 213 | Protein_2646 | recombinase family protein | - |
| ACLF6N_RS13680 (ACLF6N_13680) | fumC | 2511462..2512850 (-) | 1389 | WP_014480325.1 | class II fumarate hydratase | - |
| ACLF6N_RS13685 (ACLF6N_13685) | - | 2513017..2513907 (+) | 891 | WP_014480326.1 | LysR family transcriptional regulator | - |
| ACLF6N_RS13690 (ACLF6N_13690) | - | 2514849..2515244 (+) | 396 | WP_046160622.1 | VOC family protein | - |
| ACLF6N_RS13695 (ACLF6N_13695) | - | 2515984..2517111 (-) | 1128 | WP_014480328.1 | Rap family tetratricopeptide repeat protein | - |
| ACLF6N_RS13700 (ACLF6N_13700) | - | 2517293..2518126 (+) | 834 | Protein_2651 | ribonuclease YeeF family protein | - |
| ACLF6N_RS13705 (ACLF6N_13705) | - | 2518512..2519219 (+) | 708 | WP_014480330.1 | hypothetical protein | - |
| ACLF6N_RS13710 (ACLF6N_13710) | - | 2519233..2519520 (+) | 288 | WP_014480331.1 | hypothetical protein | - |
| ACLF6N_RS13715 (ACLF6N_13715) | - | 2519923..2520363 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| ACLF6N_RS13720 (ACLF6N_13720) | - | 2520462..2520914 (+) | 453 | WP_129134003.1 | SMI1/KNR4 family protein | - |
| ACLF6N_RS13725 (ACLF6N_13725) | cdiI | 2521011..2521370 (+) | 360 | WP_014480334.1 | ribonuclease toxin immunity protein CdiI | - |
| ACLF6N_RS13730 (ACLF6N_13730) | - | 2521475..2521954 (+) | 480 | WP_224588637.1 | hypothetical protein | - |
| ACLF6N_RS13735 (ACLF6N_13735) | - | 2522262..2522465 (-) | 204 | WP_123772462.1 | hypothetical protein | - |
| ACLF6N_RS13740 (ACLF6N_13740) | - | 2522554..2522787 (+) | 234 | WP_224588641.1 | hypothetical protein | - |
| ACLF6N_RS13745 (ACLF6N_13745) | atxG | 2523055..2523632 (+) | 578 | Protein_2660 | suppressor of fused domain protein | - |
| ACLF6N_RS13750 (ACLF6N_13750) | - | 2523742..2524032 (+) | 291 | WP_014480337.1 | contact-dependent growth inhibition system immunity protein | - |
| ACLF6N_RS13755 (ACLF6N_13755) | - | 2524940..2525106 (-) | 167 | Protein_2662 | peptidoglycan-binding domain-containing protein | - |
| ACLF6N_RS13760 (ACLF6N_13760) | - | 2525281..2525367 (+) | 87 | WP_072592549.1 | putative holin-like toxin | - |
| ACLF6N_RS13765 (ACLF6N_13765) | - | 2525654..2526129 (-) | 476 | Protein_2664 | phage tail tube protein | - |
| ACLF6N_RS13770 (ACLF6N_13770) | terS | 2526089..2526653 (-) | 565 | Protein_2665 | phage terminase small subunit | - |
| ACLF6N_RS13775 (ACLF6N_13775) | - | 2526780..2527085 (+) | 306 | WP_123772463.1 | hypothetical protein | - |
| ACLF6N_RS13780 (ACLF6N_13780) | istA | 2527658..2529205 (+) | 1548 | WP_014480339.1 | IS21 family transposase | - |
| ACLF6N_RS13785 (ACLF6N_13785) | istB | 2529202..2529960 (+) | 759 | WP_014479891.1 | IS21-like element helper ATPase IstB | - |
| ACLF6N_RS13790 (ACLF6N_13790) | - | 2529915..2530442 (-) | 528 | WP_412052440.1 | hypothetical protein | - |
| ACLF6N_RS13795 (ACLF6N_13795) | - | 2531059..2531325 (+) | 267 | WP_033881358.1 | hypothetical protein | - |
| ACLF6N_RS13800 (ACLF6N_13800) | - | 2531464..2531616 (-) | 153 | WP_049832653.1 | XtrA/YqaO family protein | - |
| ACLF6N_RS13805 (ACLF6N_13805) | - | 2531699..2531821 (-) | 123 | Protein_2672 | RusA family crossover junction endodeoxyribonuclease | - |
| ACLF6N_RS13810 (ACLF6N_13810) | - | 2531784..2532032 (-) | 249 | Protein_2673 | hypothetical protein | - |
| ACLF6N_RS13815 (ACLF6N_13815) | - | 2532177..2532407 (-) | 231 | WP_224588644.1 | hypothetical protein | - |
| ACLF6N_RS13820 (ACLF6N_13820) | - | 2532716..2532889 (-) | 174 | WP_326043410.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=1086558 ACLF6N_RS13660 WP_009967785.1 2509602..2510012(-) (nucA/comI) [Bacillus subtilis strain KFRI-P74]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=1086558 ACLF6N_RS13660 WP_009967785.1 2509602..2510012(-) (nucA/comI) [Bacillus subtilis strain KFRI-P74]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |