Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACLF6N_RS13410 Genome accession   NZ_CP177279
Coordinates   2466159..2466926 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain KFRI-P74     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2461159..2471926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6N_RS13390 (ACLF6N_13390) ppsR 2461333..2462145 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ACLF6N_RS13395 (ACLF6N_13395) ccpN 2462171..2462809 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ACLF6N_RS13400 (ACLF6N_13400) glyS 2462942..2464981 (-) 2040 WP_088272396.1 glycine--tRNA ligase subunit beta -
  ACLF6N_RS13405 (ACLF6N_13405) glyQ 2464974..2465861 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ACLF6N_RS13410 (ACLF6N_13410) recO 2466159..2466926 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  ACLF6N_RS13415 (ACLF6N_13415) yqzL 2466963..2467106 (-) 144 WP_003230053.1 YqzL family protein -
  ACLF6N_RS13420 (ACLF6N_13420) era 2467254..2468159 (-) 906 WP_003230051.1 GTPase Era -
  ACLF6N_RS13425 (ACLF6N_13425) - 2468140..2468550 (-) 411 WP_003230049.1 cytidine deaminase -
  ACLF6N_RS13430 (ACLF6N_13430) unpK 2468669..2469040 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  ACLF6N_RS13435 (ACLF6N_13435) ybeY 2469021..2469494 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  ACLF6N_RS13440 (ACLF6N_13440) pgpH 2469495..2471630 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=1086554 ACLF6N_RS13410 WP_014477369.1 2466159..2466926(-) (recO) [Bacillus subtilis strain KFRI-P74]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1086554 ACLF6N_RS13410 WP_014477369.1 2466159..2466926(-) (recO) [Bacillus subtilis strain KFRI-P74]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAACC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAGAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment