Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACLF6N_RS11755 Genome accession   NZ_CP177279
Coordinates   2176185..2176805 (+) Length   206 a.a.
NCBI ID   WP_014480105.1    Uniprot ID   A0A0D1KQW4
Organism   Bacillus subtilis strain KFRI-P74     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2171185..2181805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6N_RS11715 (ACLF6N_11715) ptsA 2172316..2172822 (-) 507 WP_029726788.1 PTS glucose transporter subunit IIA -
  ACLF6N_RS11720 (ACLF6N_11720) ypqA 2172960..2173379 (+) 420 WP_042976351.1 Hsp20/alpha crystallin family protein -
  ACLF6N_RS11725 (ACLF6N_11725) sppG 2173400..2173777 (-) 378 WP_015251832.1 YppG family protein -
  ACLF6N_RS11730 (ACLF6N_11730) yppF 2173965..2174153 (+) 189 WP_003230690.1 YppF family protein -
  ACLF6N_RS11735 (ACLF6N_11735) yppE 2174192..2174563 (-) 372 WP_021481525.1 YppE family protein -
  ACLF6N_RS11740 (ACLF6N_11740) yppD 2174609..2174854 (-) 246 WP_021481526.1 DUF5446 family protein -
  ACLF6N_RS11745 (ACLF6N_11745) sspM 2175053..2175157 (+) 105 WP_003225606.1 acid-soluble spore protein SspM -
  ACLF6N_RS11750 (ACLF6N_11750) yppC 2175182..2176144 (-) 963 WP_033881326.1 DUF2515 domain-containing protein -
  ACLF6N_RS11755 (ACLF6N_11755) recU 2176185..2176805 (+) 621 WP_014480105.1 Holliday junction resolvase RecU Machinery gene
  ACLF6N_RS11760 (ACLF6N_11760) ponA 2176827..2179571 (+) 2745 WP_004398589.1 transglycosylase domain-containing protein -
  ACLF6N_RS11765 (ACLF6N_11765) ypoC 2179647..2180141 (-) 495 WP_009967579.1 YpoC family protein -
  ACLF6N_RS11770 (ACLF6N_11770) nth 2180138..2180797 (-) 660 WP_009967580.1 endonuclease III -
  ACLF6N_RS11775 (ACLF6N_11775) dnaD 2180816..2181514 (-) 699 WP_004398499.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 24019.18 Da        Isoelectric Point: 9.6240

>NTDB_id=1086542 ACLF6N_RS11755 WP_014480105.1 2176185..2176805(+) (recU) [Bacillus subtilis strain KFRI-P74]
MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFYFWDRKE
KNGRKSIRKDELEETAYPIFLGYAPRIDYISIIEQLYFSPSSGAKG

Nucleotide


Download         Length: 621 bp        

>NTDB_id=1086542 ACLF6N_RS11755 WP_014480105.1 2176185..2176805(+) (recU) [Bacillus subtilis strain KFRI-P74]
GTGATTCGGTATCCTAACGGAAAAACATTTCAGCCGAAACATTCGGTTTCATCCCAAAACAGTCAGAAAAGGGCCCCGTC
TTACAGTAATCGCGGAATGACCCTCGAAGATGACTTAAACGAAACGAATAAGTATTATCTGACAAACCAAATTGCCGTTA
TACACAAAAAGCCGACACCTGTTCAAATTGTAAATGTCCATTATCCAAAAAGAAGTGCCGCAGTGATTAAAGAAGCTTAC
TTTAAACAATCATCGACAACAGACTACAATGGGATTTACAAAGGGCGGTATATTGATTTTGAGGCGAAAGAAACGAAAAA
CAAGACCTCTTTCCCCCTGCAGAATTTTCATGACCATCAAATCGAGCATATGAAGCAGGTAAAGGCTCAAGACGGTATTT
GTTTTGTTATTATATCCGCTTTCGACCAGGTATATTTTTTGGAAGCCGATAAGCTGTTTTATTTCTGGGACAGAAAAGAA
AAAAACGGCAGAAAATCAATTCGAAAAGATGAGTTGGAAGAAACAGCTTATCCGATTTTTCTTGGATACGCACCCAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCACCATCATCTGGTGCGAAAGGTTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KQW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

99.515

100

0.995

Multiple sequence alignment