Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KCX75_RS03070 Genome accession   NZ_CP177271
Coordinates   598821..600200 (-) Length   459 a.a.
NCBI ID   WP_412047900.1    Uniprot ID   -
Organism   Lactobacillus gasseri strain LG-145     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 593821..605200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX75_RS03050 (KCX75_03050) rlmB 594556..595311 (-) 756 WP_020807200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KCX75_RS03055 (KCX75_03055) - 595298..595741 (-) 444 WP_003649429.1 Mini-ribonuclease 3 -
  KCX75_RS03060 (KCX75_03060) cysS 595734..597146 (-) 1413 WP_020807201.1 cysteine--tRNA ligase -
  KCX75_RS03065 (KCX75_03065) gltX 597240..598739 (-) 1500 WP_003649431.1 glutamate--tRNA ligase -
  KCX75_RS03070 (KCX75_03070) radA 598821..600200 (-) 1380 WP_412047900.1 DNA repair protein RadA Machinery gene
  KCX75_RS03075 (KCX75_03075) - 600200..600751 (-) 552 WP_003649433.1 dUTP diphosphatase -
  KCX75_RS03080 (KCX75_03080) pepC 600946..602295 (+) 1350 WP_020807204.1 aminopeptidase C -
  KCX75_RS03085 (KCX75_03085) - 602362..603297 (-) 936 WP_003649435.1 AAA family ATPase -
  KCX75_RS03090 (KCX75_03090) - 603407..604084 (-) 678 WP_065169772.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  KCX75_RS03095 (KCX75_03095) - 604103..604288 (-) 186 WP_020807207.1 hypothetical protein -
  KCX75_RS03100 (KCX75_03100) - 604290..604547 (-) 258 WP_020807208.1 hypothetical protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50112.74 Da        Isoelectric Point: 8.2214

>NTDB_id=1086493 KCX75_RS03070 WP_412047900.1 598821..600200(-) (radA) [Lactobacillus gasseri strain LG-145]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEVKKVSTKATASRLMTKIGNNDPVKLNEVKAKKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
REQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNDQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLTEVNNPSAIFLDERLPNSTGSAVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEAGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKHNLTTELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1086493 KCX75_RS03070 WP_412047900.1 598821..600200(-) (radA) [Lactobacillus gasseri strain LG-145]
ATGGCAAAAGTTAAAACACGTTATAAGTGCCGTAATTGTGGTTATATTTCAGCTTCTTATTTAGGTAGATGCCCTAATTG
CGGTGCCTGGAATCAATTTGAAGAAGAAACGCAAGAAGTAAAAAAAGTATCAACCAAGGCAACTGCTAGTCGCTTAATGA
CTAAAATTGGAAATAATGATCCAGTAAAATTAAATGAAGTAAAAGCTAAAAAAGAAAAAAGAATTGTAACTCCTTTTGAA
GAACTAAATCGAGTTCTAGGAGGCGGAATAGTTCCGGGTTCTTTAGTTTTAATTGGTGGAGATCCTGGAATTGGTAAGTC
AACTTTGATGCTTCAAATTACCGGTGCTTTAGCTAAAGAGCATAGTGTTTTATATGTTTCAGGAGAAGAGTCGGCTAGTC
AGATAAAGATGCGAGCTGATCGTTTAGGTGTAAGCAACAGCGGAATTTTGCTTTATCCTGAAACCAATATGCAGAATATT
CGTGAACAGATTGATGAAATTAAGCCGGATTTTTTGGTAATCGACTCTATCCAAACAATGAATGAGCCATCTCTTGATTC
GATGGTTGGATCAGCTTCTCAAGTTCGAGAAGTTACGAGTGAGTTAATGAAGATTGCCAAGAATGATCAGATTACTACTT
TTGTTATTGGACATGTGACTAAAGAAGGTGCAATTGCTGGCCCTAAGATTATGGAACATATGGTTGATACTGTCCTTTAC
TTTGAAGGGGATGGCCACCATTCATATCGAATTTTAAGGTCTGTCAAAAATCGTTTTGGTGCAGCTAATGAGATTGGTAT
GTTTGAAATGAAAAATGAAGGACTAACAGAAGTAAATAATCCTTCAGCGATTTTCTTAGACGAACGTTTACCAAATTCTA
CAGGTTCAGCAGTTGTCGTTTCGCTTGAAGGAACAAGGCCACTTCTAGCCGATATTCAAGCTTTGGTAACTCCAACTGCC
TTTGGCTATGCTAAAAGAACTACGTCGGGGTTAGATTTCAATCGTGTAGCCTTATTATTAGCGGTTTTAGAAAAACGTGG
TAACTTAATGTTACAAAATCAAGATGCATTTTTAACGGCAACAGGTGGAATTAAATTAAATGAGCCAGCGATTGACTTGG
CAATTTGTATGGCTGTAGCTTCTAGTTATAAAAATAAAGAAATTTCTTCTACTGATTGTTTTGTAGGCGAAGCTGGCTTA
ACCGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAAGCTGCTAAAGTAGGCTTTAAGCGAATTTTTAT
TCCAAAACATAATTTAACTACTGAGCTTAAGAATAATTCTGAGATTGAAGTAATTGGTGTAGCAAGTTTGCCGCAAGCTT
TAAAACTGGTTTTTAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

AAA domain (AAA_25)

(68-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

60.044

99.782

0.599
  radA Streptococcus mitis NCTC 12261

60.044

99.782

0.599
  radA Streptococcus pneumoniae Rx1

60.044

99.782

0.599
  radA Streptococcus pneumoniae D39

60.044

99.782

0.599
  radA Streptococcus pneumoniae R6

60.044

99.782

0.599
  radA Streptococcus pneumoniae TIGR4

60.044

99.782

0.599
  radA Bacillus subtilis subsp. subtilis str. 168

55.702

99.346

0.553

Multiple sequence alignment