Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACLB1C_RS05815 Genome accession   NZ_CP177244
Coordinates   1226988..1228493 (-) Length   501 a.a.
NCBI ID   WP_129512998.1    Uniprot ID   -
Organism   Burkholderia cepacia strain zafu-111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1221988..1233493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB1C_RS05790 (ACLB1C_05790) - 1223015..1223650 (-) 636 WP_048246968.1 LON peptidase substrate-binding domain-containing protein -
  ACLB1C_RS05795 (ACLB1C_05795) rapZ 1223678..1224586 (-) 909 WP_129512999.1 RNase adapter RapZ -
  ACLB1C_RS05800 (ACLB1C_05800) hprK 1224662..1225630 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  ACLB1C_RS05805 (ACLB1C_05805) - 1225760..1226272 (-) 513 WP_048246965.1 PTS sugar transporter subunit IIA -
  ACLB1C_RS05810 (ACLB1C_05810) hpf 1226470..1226829 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLB1C_RS05815 (ACLB1C_05815) rpoN/rpoN1 1226988..1228493 (-) 1506 WP_129512998.1 RNA polymerase factor sigma-54 Machinery gene
  ACLB1C_RS05820 (ACLB1C_05820) lptB 1228654..1229430 (-) 777 WP_193099116.1 LPS export ABC transporter ATP-binding protein -
  ACLB1C_RS05825 (ACLB1C_05825) lptA 1229427..1230092 (-) 666 WP_412023001.1 lipopolysaccharide transport periplasmic protein LptA -
  ACLB1C_RS05830 (ACLB1C_05830) lptC 1230113..1230727 (-) 615 WP_412023002.1 LPS export ABC transporter periplasmic protein LptC -
  ACLB1C_RS05835 (ACLB1C_05835) - 1230731..1231267 (-) 537 WP_129512994.1 KdsC family phosphatase -
  ACLB1C_RS05840 (ACLB1C_05840) kdsD 1231267..1232250 (-) 984 WP_129512993.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54823.90 Da        Isoelectric Point: 4.8740

>NTDB_id=1086422 ACLB1C_RS05815 WP_129512998.1 1226988..1228493(-) (rpoN/rpoN1) [Burkholderia cepacia strain zafu-111]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVASDGSLIAQTPPAQNTEPSA
ANSSSQSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPSELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1086422 ACLB1C_RS05815 WP_129512998.1 1226988..1228493(-) (rpoN/rpoN1) [Burkholderia cepacia strain zafu-111]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTCCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGTTGCGCGTTGCGTCGGACGGATCGCTGATCGCGCAGACGCCCCCCGCGCAGAACACCGAGCCCAGCGCG
GCGAACAGCAGCAGCCAGTCGAGCGATCGCGCAGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGTCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGATCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATGGTGAAGAAGGTCGGCCAGGGATGGCTCGCGGAACTCAATCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCACAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.984

100

0.687

Multiple sequence alignment