Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACK56M_RS24925 Genome accession   NZ_CP177209
Coordinates   5372003..5373373 (-) Length   456 a.a.
NCBI ID   WP_003251626.1    Uniprot ID   I7AVL1
Organism   Pseudomonas sp. s4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5367003..5378373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK56M_RS24905 (ACK56M_24905) - 5367227..5367424 (-) 198 WP_013974210.1 YbdD/YjiX family protein -
  ACK56M_RS24910 (ACK56M_24910) - 5367438..5369504 (-) 2067 WP_015271794.1 carbon starvation CstA family protein -
  ACK56M_RS24915 (ACK56M_24915) - 5369683..5370045 (+) 363 WP_013974212.1 PilZ domain-containing protein -
  ACK56M_RS24920 (ACK56M_24920) - 5370309..5371826 (+) 1518 WP_013974213.1 nucleobase:cation symporter-2 family protein -
  ACK56M_RS24925 (ACK56M_24925) radA 5372003..5373373 (-) 1371 WP_003251626.1 DNA repair protein RadA Machinery gene
  ACK56M_RS24930 (ACK56M_24930) mscL 5373550..5373969 (+) 420 WP_015271796.1 large-conductance mechanosensitive channel protein MscL -
  ACK56M_RS24935 (ACK56M_24935) - 5374104..5374880 (-) 777 WP_015271797.1 ferredoxin--NADP reductase -
  ACK56M_RS24940 (ACK56M_24940) - 5375093..5375800 (+) 708 WP_015271798.1 autoinducer binding domain-containing protein -
  ACK56M_RS24945 (ACK56M_24945) - 5375897..5377021 (+) 1125 WP_015271799.1 methyltransferase -
  ACK56M_RS24950 (ACK56M_24950) - 5377338..5377493 (-) 156 WP_013974218.1 DUF2474 domain-containing protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48741.18 Da        Isoelectric Point: 6.8985

>NTDB_id=1086187 ACK56M_RS24925 WP_003251626.1 5372003..5373373(-) (radA) [Pseudomonas sp. s4]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1086187 ACK56M_RS24925 WP_003251626.1 5372003..5373373(-) (radA) [Pseudomonas sp. s4]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAGTGCGGTGCGACCTTCCCCAAATGGGCCGGCCAGTGTGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAGACCATGATCGAAAGCGGCGGTGCCGCTGCGCCCAGTAGCGGCCGTGCGGGCTGGA
CCGGGCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTCGAGGAGATCCCGCGCTTCACCACCAGCAGCACCGAA
CTGGACCGCGTGCTGGGCGGTGGCCTGGTGGATGGCTCGGTGGTGTTGATCGGTGGTGACCCCGGCATCGGCAAATCGAC
CATCCTGCTGCAGACCTTGTGCAACATTGCCGTGGGCATGCCGGCGCTGTATGTGACCGGCGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCCCGGCGCCTGGGCCTGCCCCAGGACCAGCTCAAGGTGATGACCGAAACCTGCATCGAGACCATCATC
GCCACGGCCCGCGTCGAGAAACCGCGGGTCATGGTGATCGACTCGATCCAGACCATTTTCACCGAGCAGCTGCAATCGGC
ACCCGGCGGCGTGGCCCAGGTGCGCGAAAGCACGGCGTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATCTTCC
TGGTCGGCCATGTGACCAAGGAAGGTTCGCTGGCCGGCCCGCGGGTACTCGAGCACATGGTGGACACCGTGCTGTATTTC
GAGGGCGAGTCCGATGGCCGCCTGCGCCTGCTGCGGGCAGTCAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGTGTGTT
CGGCATGACCGACCGGGGCCTGAAGGAGGTGTCCAACCCCTCGGCGATCTTCCTCAACCGCACCCAGGAGGAAGTACCGG
GCAGCGTGGTGATGGCGACCTGGGAGGGCACCCGGCCGATGCTGGTGGAGGTGCAGGCGCTGGTCGACGACAGCCACCTG
GCCAACCCGCGCCGGGTGACCTTGGGCCTTGACCAGAATCGCCTGGCAATGTTGCTGGCGGTGCTGCACCGCCACGGCGG
TATTCCCACCCATGACCAGGACGTGTTCCTCAACGTGGTGGGTGGGGTGAAGGTGCTGGAAACCGCTTCTGACCTGGCGT
TGCTGGCGGCGGTGATGTCCAGCCTGCGCAACCGGCCCCTGGCCCATGGCCTGCTGGTGTTTGGCGAGATCGGCCTGTCT
GGCGAGGTACGGCCGGTGCCCAGTGGTCAGGAGCGTTTGAAGGAGGCCGCCAAGCATGGTTTCAAGCGGGCCATCGTGCC
CAAGGGCAATGCCCCGAAAGAGGCTCCGGCCGGGTTGCAGGTGATTGCCGTTACCCGGCTGGAGCAGGCTCTGGATGCGT
TGTTCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7AVL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461

Multiple sequence alignment